公开(公告)号：US07687267B2

公开(公告)日：2010-03-30

申请号：US11529964

申请日：2006-09-30

申请人： Yong Huang

发明人： Yong Huang

IPC分类号： C12N15/87

CPC分类号： C12Q1/025 ,  C12M23/12 ,  C12M35/02 ,  C12N15/87

摘要： Transfecting biology cells with nucleic acid molecules (DNA, siRNA) is an essential prerequisite in elucidating how genes function in complex cellular context and how their activities could be modulated for therapeutic intervention. Traditionally studies are carried out on a low throughput gene-by-gene scale, which has created a huge bottleneck in functional genomic study and drug discovery. Development of high-throughput cell transfection technology will permit functional analysis of massive number of genes and how their activities could be modulated by chemical or biological entities inside cells. This invention describes design, construction of device and apparatus for high throughput effective cell transfection. Procedures and protocols for using the device and apparatus are also described in the application. Novel methods of using the device in cell-based assays are also disclosed.

摘要翻译： 用核酸分子（DNA，siRNA）转染生物细胞是阐明基因如何在复杂细胞环境中起作用的必要先决条件，以及它们的活性如何被调节用于治疗干预。 传统的研究是在低通量基因按基因量表上进行的，这在功能基因组研究和药物发现中造成了巨大的瓶颈。 开发高通量细胞转染技术将允许大量基因的功能分析，以及它们的活性如何被细胞内的化学或生物实体调节。 本发明描述了用于高通量有效细胞转染的装置和装置的设计，构建。 在应用中还描述了使用该设备和设备的过程和协议。 还公开了在基于细胞的测定中使用该装置的新方法。

公开(公告)号：US07668870B1

公开(公告)日：2010-02-23

申请号：US10825502

申请日：2004-04-15

申请人： Roy Sykes ,  Joseph Zusman ,  Sikun Lan ,  Kent Frazier ,  Tyler Young ,  Yong Huang ,  Stanislav Solari

发明人： Roy Sykes ,  Joseph Zusman ,  Sikun Lan ,  Kent Frazier ,  Tyler Young ,  Yong Huang ,  Stanislav Solari

IPC分类号： G06F12/00

CPC分类号： G06F17/3089

摘要： A method for updating web pages on a web server without republishing the web pages involves providing on a production database server a live version of one or more business data tables containing information used to populate web pages on a production web server and storing in a quality assurance database server a quality assurance version of the business data tables that can be used to populate pages on a quality assurance web server. A maker at a business workstation can access the quality assurance database server from time to time and enter a change to data on the quality assurance version of the business data tables. Thereafter a checker at a business workstation accesses the quality assurance database server to review and approve or reject the changes to the data on the quality assurance version of the business data tables, and if the checker approves the changes, the changes to the data on the quality assurance version of the business data tables are replicated from the quality assurance database server to the live version of the business data tables on the production database server.

摘要翻译： 一种用于在不重新发布网页的情况下更新网页服务器上的网页的方法包括在生产数据库服务器上提供一个或多个业务数据表的实时版本，其中包含用于在生产web服务器上填充网页并存储在质量保证中的信息 数据库服务器可以用于在质量保证Web服务器上填充页面的业务数据表的质量保证版本。 业务工作站的制造商可以不时访问质量保证数据库服务器，并对业务数据表的质量保证版本的数据进行更改。 此后，业务工作站的检查员访问质量保证数据库服务器以审查和批准或拒绝对业务数据表的质量保证版本的数据的更改，如果检查者批准更改，则对数据的更改 业务数据表的质量保证版本从质量保证数据库服务器复制到生产数据库服务器上的业务数据表的实时版本。

公开(公告)号：US20090193481A1

公开(公告)日：2009-07-30

申请号：US12419816

申请日：2009-04-07

申请人： Yong Huang

发明人： Yong Huang

IPC分类号： H04N7/173

CPC分类号： H04N21/234 ,  H04L12/14 ,  H04L12/1471 ,  H04L12/185 ,  H04L65/4076 ,  H04N7/17318 ,  H04N21/2221 ,  H04N21/25816 ,  H04N21/26216 ,  H04N21/4384 ,  H04N21/4788 ,  H04N21/6125 ,  H04N21/64322

摘要： A method for realizing video live broadcast, includes: receiving a request for creating conversation from a user equipment, the request carrying indication information for identifying video live broadcast BTV service packet, the BTV packet includes several channel program with the same authentication attribute and the same accounting attribute; sending BTV service control information corresponding to BTV service packet to a network marginal equipment corresponding to the user equipment UE; creating the conversation between an IPTV server and the user equipment UE according to the request; sending the channel program that the UE need access to the UE according to the BTV service control information. Corresponding equipment and system also are provided. The invention can ensure flexible authentication and accounting capability, meanwhile it also ensure the user can switch channel quickly according to the present invention.

摘要翻译： 一种用于实现视频直播的方法，包括：从用户设备接收用于创建对话的请求，所述请求携带用于识别视频直播BTV服务分组的指示信息，所述BTV分组包括具有相同认证属性的相同的多个频道节目 会计属性; 向对应于用户设备UE的网络边缘设备发送对应于BTV业务分组的BTV业务控制信息; 根据请求创建IPTV服务器与用户设备UE之间的对话; 根据BTV业务控制信息发送UE需要接入UE的信道节目。 还提供相应的设备和系统。 本发明可以确保灵活的认证和计费能力，同时还保证用户可以根据本发明快速切换通道。

公开(公告)号：US07520210B2

公开(公告)日：2009-04-21

申请号：US11528035

申请日：2006-09-27

申请人： Michael Gregory Theodore, Jr. ,  Brian Kelm ,  Alfred Lim Poy ,  Yong Huang ,  Kanwal Bhatia

发明人： Michael Gregory Theodore, Jr. ,  Brian Kelm ,  Alfred Lim Poy ,  Yong Huang ,  Kanwal Bhatia

IPC分类号： F01B3/00 ,  F01B31/10 ,  F04B27/08

CPC分类号： F04B27/109 ,  F04B27/1054

摘要： An oil separator for a compressor is shown, wherein an oil separating efficiency is maximized and a material cost, a weight, and an assembly time are minimized.

摘要翻译： 示出了用于压缩机的油分离器，其中油分离效率最大化并且材料成本，重量和组装时间最小化。

公开(公告)号：US20080066202A1

公开(公告)日：2008-03-13

申请号：US11856958

申请日：2007-09-18

申请人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

发明人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

IPC分类号： C12N15/84 ,  C12N15/82

CPC分类号： C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

摘要翻译： 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。

公开(公告)号：US20050007497A1

公开(公告)日：2005-01-13

申请号：US10847205

申请日：2004-05-17

申请人： Yong Huang ,  Patricia Chiang ,  Lucas Hui ,  Kwong Goh

发明人： Yong Huang ,  Patricia Chiang ,  Lucas Hui ,  Kwong Goh

IPC分类号： H04N5/52 ,  H04N9/64 ,  H04N9/68 ,  H04N9/77

CPC分类号： H04N9/68 ,  H04N9/77

摘要： A method of colour saturation compensation in a video signal is disclosed. The method includes the steps of: processing a luminance signal component of the video signal; determining whether the processing of the luminance signal results in a change in chrominance saturation; if the step of determining reveals that the chrominance saturation has changed, applying a chrominance compensation signal to the chrominance signals to counteract the effects of chrominance saturation, wherein said step of applying a compensation signal include the steps of: generating a compensation signal which is dependent on hue and luminance ratio (output luminance signal/input luminance signal). Apparatus for performing the method is also disclosed.

摘要翻译： 公开了一种视频信号中色彩饱和补偿的方法。 该方法包括以下步骤：处理视频信号的亮度信号分量; 确定亮度信号的处理是否导致色度饱和度的变化; 如果确定步骤显示色度饱和度已经改变，则将色度补偿信号施加到色度信号以抵消色度饱和的影响，其中所述施加补偿信号的步骤包括以下步骤：产生依赖的补偿信号 色相和亮度比（输出亮度信号/输入亮度信号）。 还公开了用于执行该方法的装置。

公开(公告)号：US06591735B2

公开(公告)日：2003-07-15

申请号：US09782779

申请日：2001-02-13

申请人： Lavlesh Sud ,  Mirza Qadir M. Baig ,  Srinivas S. Pitla ,  Vipen Khetarpal ,  Yong Huang

发明人： Lavlesh Sud ,  Mirza Qadir M. Baig ,  Srinivas S. Pitla ,  Vipen Khetarpal ,  Yong Huang

IPC分类号： F01B310

CPC分类号： F04B27/0878 ,  F04B27/109

摘要： A swashplate type compressor that includes one or more pistons having an extra support surface for receiving a side load is provided. In a first preferred embodiment of the piston, a rotational restrictor that overlaps the head region is utilized as the extra support surface. In a second preferred embodiment, a support projection, separate from the head region, is utilized as the extra support surface. The extra support surface of the piston of the present invention receives a portion of the side load exerted on the piston, thereby relieving some of stress applied to the head region.

公开(公告)号：US06402480B1

公开(公告)日：2002-06-11

申请号：US09747624

申请日：2000-12-22

申请人： Lavlesh Sud ,  Vipen Khetarpal ,  Shane A. Harte ,  David H. Herder ,  Yong Huang

发明人： Lavlesh Sud ,  Vipen Khetarpal ,  Shane A. Harte ,  David H. Herder ,  Yong Huang

IPC分类号： F04B126

CPC分类号： F04B27/109

摘要： A variable displacement swash plate type compressor which incorporates a lubrication passage formed in the drive shaft, wherein the lubrication passage provides fluid communication between a discharge chamber and a crank chamber. The lubrication passage maximizes the low of refrigerant gas and lubricating oil to the crank chamber under all operating conditions providing cooling and lubrication to the internal moving components in the crank chamber. The lubrication passage facilitates the efficient flow of lubricating oil from the discharge chamber to the crank chamber.

摘要翻译： 一种可变排量斜盘式压缩机，其包括形成在所述驱动轴中的润滑通道，其中所述润滑通道在排出室和曲柄室之间提供流体连通。 润滑通道在所有操作条件下将制冷剂气体和润滑油的低度最大化到曲柄室，从而为曲柄室中的内部移动部件提供冷却和润滑。 润滑通道有助于润滑油从排放室到曲柄室的有效流动。

公开(公告)号：US06318972B1

公开(公告)日：2001-11-20

申请号：US09538190

申请日：2000-03-30

申请人： Yong Huang ,  Laviesh Sud ,  Shane Harte

发明人： Yong Huang ,  Laviesh Sud ,  Shane Harte

IPC分类号： F04B112

CPC分类号： F04B39/1073 ,  F04B27/1009 ,  Y10T137/7892

摘要： A compressor (10) for a vehicular air conditioning system includes a cylinder block (12) having a bore (14). A piston (78) is slidingly received in the bore (14). A cylinder head (16) is mounted on the cylinder block (12) and includes a suction chamber (20) and a discharge chamber (22). A valve plate (18) is provided between cylinder block (12) and the cylinder head (16). The valve plate (18) includes a suction port (26) and a discharge port (35). A suction valve (28) cooperates with the suction port (26) to permit refrigerant to enter the bore (14) from the suction chamber (20). The suction valve (28) includes a terminating portion (28A) and a flexing portion (28B). A recess (32) is formed in the cylinder block (12) adjacent the flexing portion (28B) of the suction valve (28). The recess (32) provides a linear clamping line (C) on the suction valve (28) between the valve plate (18) and the cylinder block (12).

摘要翻译： 一种用于车辆空调系统的压缩机（10）包括具有孔（14）的气缸体（12）。 活塞（78）滑动地容纳在孔（14）中。 气缸盖（16）安装在气缸体（12）上并且包括吸入室（20）和排出室（22）。 在气缸体（12）和气缸盖（16）之间设有阀板（18）。 阀板（18）包括吸入口（26）和排出口（35）。 吸入阀（28）与吸入口（26）配合以允许制冷剂从吸入室（20）进入孔（14）。 吸入阀（28）包括终止部分（28A）和弯曲部分（28B）。 在与吸入阀（28）的弯曲部（28B）相邻的气缸体（12）中形成有凹部（32）。 凹部（32）在阀板（18）和气缸体（12）之间的吸入阀（28）上提供线性夹紧线（C）。

公开(公告)号：US6097869A

公开(公告)日：2000-08-01

申请号：US343569

申请日：1999-06-30

申请人： Kok Wai Chang ,  Yong Huang

发明人： Kok Wai Chang ,  Yong Huang

IPC分类号： G02B6/255 ,  G02B6/26 ,  G02B6/34 ,  G02B6/02

CPC分类号： G02B6/2713 ,  G02B6/02042 ,  G02B6/2552 ,  G02B6/2746 ,  G02B6/2766 ,  G02B6/29332

摘要： A multiple port reflection based circulator using a thermally expanded multiple core fiber. The circulator includes optical components to separate, rotate, and combine orthogonally polarized components of light in order to propagate light in one direction among consecutive cores of the fiber. The first and last cores of the fiber also being consecutive cores. The thermally expanded multiple core fiber enabling coupling to the circulator without the use of lenses.

摘要翻译： 使用热扩展多芯光纤的多端口反射式循环器。 循环器包括用于分离，旋转和组合光的正交极化分量的光学部件，以便在光纤的连续芯之间沿一个方向传播光。 光纤的第一个和最后一个核心也是连续的核心。 热膨胀的多芯光纤能够在不使用透镜的情况下耦合到循环器。