摘要:
A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell.A method of expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carrier and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized by the RNA polymerase.
摘要:
An efficient, versatile and simple expression system which confers cap-independent translation to prokaryotic RNAs in eukaryotic cells has been described. The utility of recombinant vaccinia virus for this purpose has been illustrated.
摘要:
The present invention relates to a eukaryotic cell line which expresses a foreign RNA polymerase gene. The invention further relates to a method of expressing a foreign protein in a eukaryotic environment utilizing the cell line. The present invention allows for the expression of a foreign protein in a eukaryotic cell without requiring transfecting or infecting the cell with a vector carrying the RNA polymerase gene.
摘要:
A stable, post-foaming gel shower product comprising a soap-free, surfactant-based gel composition consisting essentially of a major amount of water, about 3-23% by weight of a water-soluble anionic alkali metal C.sub.10 -C.sub.16 alkyl ether sulfate surfactant, about 1-24% by weight of a water dispersible ethoxylated fatty alcohol or fatty ester, about 2-4% of isopropyl myristate, about 1-10% of a mono- or disaccharide and about 5-20% by weight of a saturated aliphatic hydrocarbon foaming agent having 4 to 5 carbon atoms as an integral part of the gel structure, the anionic surfactant and the ethoxylated fatty alcohol or ester being present in a weight ratio of 4:1 to about 1:4.