公开(公告)号：US20050019808A1

公开(公告)日：2005-01-27

申请号：US10888143

申请日：2004-07-09

申请人： Ann Palmenberg ,  Aleksey Aminev ,  Rachel Groppo

发明人： Ann Palmenberg ,  Aleksey Aminev ,  Rachel Groppo

IPC分类号： C07K14/085 ,  C12N15/67 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C07K14/005 ,  A61K2039/5256 ,  C12N15/67 ,  C12N2770/32222 ,  C12N2770/32243

摘要： The invention relates to compositions, specifically novel nucleic acid constructs encoding a cardiovirus 2A polypeptide operably linked to suitable promoters. Also, disclosed are methods whereby the nucleic acid constructs are introduced into cells or cell free systems to regulate cellular mRNA transcription and cap-dependent or internal ribosomal entry site (IRES)-dependent mRNA translation.

摘要翻译： 本发明涉及组合物，特别是编码与合适的启动子可操作地连接的心脏病毒2A多肽的新型核酸构建体。 还公开了将核酸构建体引入细胞或无细胞系统以调节细胞mRNA转录和帽依赖性或内部核糖体进入位点（IRES） - 依赖性mRNA翻译的方法。

公开(公告)号：US07947493B2

公开(公告)日：2011-05-24

申请号：US10888143

申请日：2004-07-09

申请人： Ann C. Palmenberg ,  Aleksey G. Aminev ,  Rachel P. Groppo

发明人： Ann C. Palmenberg ,  Aleksey G. Aminev ,  Rachel P. Groppo

IPC分类号： C12N15/00 ,  C12N15/10 ,  C12N15/11 ,  C12N15/34

CPC分类号： C07K14/005 ,  A61K2039/5256 ,  C12N15/67 ,  C12N2770/32222 ,  C12N2770/32243

摘要： The invention relates to compositions, specifically novel nucleic acid constructs encoding a cardiovirus 2A polypeptide operably linked to suitable promoters. Also, disclosed are methods whereby the nucleic acid constructs are introduced into cells or cell free systems to regulate cellular mRNA transcription and cap-dependent or internal ribosomal entry site (IRES)-dependent mRNA translation.

摘要翻译： 本发明涉及组合物，特别是编码与合适的启动子可操作地连接的心脏病毒2A多肽的新型核酸构建体。 还公开了将核酸构建体引入细胞或无细胞系统以调节细胞mRNA转录和帽依赖性或内部核糖体进入位点（IRES） - 依赖性mRNA翻译的方法。

公开(公告)号：US20170204469A1

公开(公告)日：2017-07-20

申请号：US15410401

申请日：2017-01-19

申请人： Toshiba Medical Systems Corporation

发明人： Eiichi AKAHOSHI ,  Emi NOZAKI ,  Mitsuko ISHIHARA

IPC分类号： C12Q1/68 ,  B01L7/00 ,  C12N15/86

CPC分类号： C12Q1/6886 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/18 ,  C12N15/63 ,  C12N15/79 ,  C12N15/86 ,  C12N2710/16143 ,  C12N2710/22043 ,  C12N2770/32243 ,  C12Q1/6825 ,  C12Q2600/158

摘要： According to one embodiment, a reporter vector includes a first and a second reporter gene expression unit and a replication initiation sequence. The first reporter gene expression unit includes a promoter sequence of a cell characteristics marker, a first reporter gene, and a first transcription termination sequence. The second reporter gene expression unit includes a promoter sequence exhibiting constitutive activity, a second reporter gene, a bicistronic expression sequence, a replication initiation protein gene, and a second transcription termination sequence. The replication initiation sequence binds to a replication initiation protein, thereby initiating replication of the reporter vector.

公开(公告)号：US20050118566A1

公开(公告)日：2005-06-02

申请号：US10478434

申请日：2002-05-23

申请人： Nicolas Escriou ,  Sylvie Van Der Werf ,  Marco Vignuzzi ,  Sylvie Gerbaud

发明人： Nicolas Escriou ,  Sylvie Van Der Werf ,  Marco Vignuzzi ,  Sylvie Gerbaud

IPC分类号： C12N15/09 ,  A61K39/12 ,  A61K39/39 ,  A61P31/14 ,  C12N7/01 ,  C12N15/41 ,  C12N15/86 ,  C12Q1/70 ,  C07H21/02 ,  C12N7/00 ,  C12Q1/68

CPC分类号： C12N15/86 ,  A61K2039/53 ,  C12N2770/32243 ,  C12N2820/60 ,  C12N2840/203

摘要： The present invention relates to replicons or self-replicating RNA molecules, derived from the genome of cardioviruses and aphtoviruses, which can be used to express heterologous proteins in animal cells. When injected in an animal host, for example in the form of naked RNA, these replicons permit the translation of the encoded heterologous protein. If the encoded heterologous protein is a foreign antigen, these replicons induce an immune response against the encoded heterologous protein. The invention uses cardiovirus and aphtovirus genomes to construct these replicons. The invention demonstrates that these replicons, when injected as naked RNA, can induce immune responses against a replicon-encoded heterologous protein in an animal recipient without the help of any kind of carrier or adjuvant.

摘要翻译： 本发明涉及可以用于在动物细胞中表达异源蛋白质的来源于心脏病毒和致病原体的基因组的复制子或自我复制的RNA分子。 当注射在动物宿主中时，例如以裸RNA的形式，这些复制子允许编码的异源蛋白质的翻译。 如果编码的异源蛋白是外源抗原，则这些复制子诱导针对编码的异源蛋白的免疫应答。 本发明使用心脏病毒和aphtovirus基因组构建这些复制子。 本发明证明，当以裸RNA注射时，这些复制子可以在没有任何种类的载体或佐剂的帮助下诱导针对动物受体中复制子编码的异源蛋白的免疫应答。

公开(公告)号：US20030077251A1

公开(公告)日：2003-04-24

申请号：US10152040

申请日：2002-05-22

发明人： Nicolas Escriou ,  Sylvie Van Der Werf ,  Marco Vignuzzi ,  Sylvie Gerbaud

IPC分类号： A61K048/00 ,  C07H021/02 ,  C12N015/86 ,  C12N007/01

CPC分类号： C12N15/86 ,  A61K2039/53 ,  C12N2770/32243 ,  C12N2820/60 ,  C12N2840/203

摘要： The present invention relates to replicons or self-replicating RNA molecules, derived from the genome of cardioviruses and aphtoviruses, which can be used to express heterologous proteins in animal cells. When injected in an animal host, for example in the form of naked RNA, these replicons permit the translation of the encoded heterologous protein. If the encoded heterologous protein is a foreign antigen, these replicons induce an immune response against the encoded heterologous protein. The invention uses cardiovirus and aphtovirus genomes to construct these replicons. The invention demonstrates that these replicons, when injected as naked RNA, can induce immune responses against a replicon-encoded heterologous protein in an animal recipient without the help of any kind of carrier or adjuvant.

摘要翻译： 本发明涉及可以用于在动物细胞中表达异源蛋白质的来源于心脏病毒和致病原体的基因组的复制子或自我复制的RNA分子。 当注射在动物宿主中时，例如以裸RNA的形式，这些复制子允许编码的异源蛋白质的翻译。 如果编码的异源蛋白是外源抗原，则这些复制子诱导针对编码的异源蛋白的免疫应答。 本发明使用心脏病毒和aphtovirus基因组构建这些复制子。 本发明证明，当以裸RNA注射时，这些复制子可以在没有任何种类的载体或佐剂的帮助下诱导针对动物受体中复制子编码的异源蛋白的免疫应答。

公开(公告)号：US5135855A

公开(公告)日：1992-08-04

申请号：US376687

申请日：1989-07-07

申请人： Bernard Moss ,  Thomas Fuerst ,  Orna Elroy-Stein

发明人： Bernard Moss ,  Thomas Fuerst ,  Orna Elroy-Stein

IPC分类号： C12N7/00 ,  C12N7/01 ,  C12N9/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12N15/85 ,  C12N15/863 ,  C12P21/00 ,  C12R1/91

CPC分类号： C12N15/86 ,  C12N15/10 ,  C12N15/67 ,  C12N15/85 ,  C12N9/1033 ,  C12N9/1247 ,  C12N2710/24143 ,  C12N2770/32243

摘要： An efficient, versatile and simple expression system which confers cap-independent translation to prokaryotic RNAs in eukaryotic cells has been described. The utility of recombinant vaccinia virus for this purpose has been illustrated.

摘要翻译： 已经描述了赋予真核细胞中与原核RNA无关的翻译的有效，通用和简单的表达系统。 已经说明了重组痘苗病毒用于此目的的用途。