公开(公告)号：US10550363B1

公开(公告)日：2020-02-04

申请号：US16454865

申请日：2019-06-27

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10532324B1

公开(公告)日：2020-01-14

申请号：US16540606

申请日：2019-08-14

Applicant: Inscripta, Inc.

Inventor： Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Richard Fox ,  Matthew Estes ,  Bruce Chabansky

IPC: B01D63/08 ,  B01D25/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20190194650A1

公开(公告)日：2019-06-27

申请号：US16295393

申请日：2019-03-07

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US10287575B2

公开(公告)日：2019-05-14

申请号：US15948798

申请日：2018-04-09

Applicant: The Regents of the University of Colorado ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US09982279B1

公开(公告)日：2018-05-29

申请号：US15632001

申请日：2017-06-23

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/85 ,  C12N9/22 ,  C12N5/10 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20250066818A1

公开(公告)日：2025-02-27

申请号：US18945973

申请日：2024-11-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US12195749B2

公开(公告)日：2025-01-14

申请号：US17692069

申请日：2022-03-10

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220195464A1

公开(公告)日：2022-06-23

申请号：US17692069

申请日：2022-03-10

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N15/81 ,  C12N15/70 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11306327B1

公开(公告)日：2022-04-19

申请号：US17554736

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220033829A1

公开(公告)日：2022-02-03

申请号：US17387780

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Tyson Shepherd ,  Andrew Garst ,  Daniel Held ,  Eric Abbate

IPC: C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for using sets of short, curated peptide barcodes to track nucleic acid-guided edits and the translated proteins that result from the edits as well as to create in vitro pathways.