Cellulase variants
    51.
    发明授权
    Cellulase variants 失效
    纤维素酶变体

    公开(公告)号:US6114296A

    公开(公告)日:2000-09-05

    申请号:US57088

    申请日:1998-04-08

    摘要: The present invention provides variants of a parent cellulase, such as, e.g., a cellulase classified in family 45 such as a Humicola insolens 43 kD endoglucanase, comprising a cellulose binding domain (CBD), a catalytically active domain (CAD), and a region linking the CBD and CAD (the linking region), wherein one or more amino acid residues of the CBD, CAD, or linking region is deleted or substituted by one or more amino acid residues and/or one or more amino acids are added to the linking region and/or another CBD is added at the opposite end of the CAD. Such variants have improved properties as regards, e.g., alkaline activity, compatibility with detergent composition ingredients, particular soil removal, color clarification, defuzzing, depilling, harshness reduction, and sensitivity to anionic surfactants. Such variants are useful, e.g., in detergent compositions, for textile treatment, in paper pulp processing, for animal feed, and for stone-washing of jeans.

    摘要翻译: 本发明提供亲本纤维素酶的变体,例如分类在家族45中的纤维素酶,例如包含纤维素结合结构域(CBD),催化活性结构域(CAD)和区域的区域的腐质霉43kD内切葡聚糖酶 连接CBD和CAD(连接区),其中将CBD,CAD或连接区的一个或多个氨基酸残基缺失或被一个或多个氨基酸残基和/或一个或多个氨基酸加入到 连接区域和/或另一个CBD被添加在CAD的相对端。 这些变体具有改善的性能,例如碱活性,与洗涤剂组合物成分的相容性,特定的污垢去除,颜色澄清,去污,去毛刺,粗糙度降低以及对阴离子表面活性剂的敏感性。 这样的变体例如在洗涤剂组合物中用于纺织品处理,纸浆加工,动物饲料和牛仔裤的石洗。

    Enzyme exhibiting cellulase activity
    55.
    发明授权
    Enzyme exhibiting cellulase activity 失效
    显示纤维素酶活性的酶

    公开(公告)号:US5916796A

    公开(公告)日:1999-06-29

    申请号:US862588

    申请日:1992-07-27

    摘要: This invention relates to an enzyme which exhibits cellulase activity and is producible by a strain of Bacillus sp., NCIMB 40250, or a related Bacillus sp. strain, or a derivative of said cellulase. This invention also relates to an enzyme which comprises a core region derived from an endoglucanase combined with a cellulose-binding domain derived from another cellulase, or which comprises a core region derived from another cellulase combined with a cellulose-binding domain derived from an endoglucanase.

    摘要翻译: PCT No.PCT / DK91 / 00013 Sec。 371日期:1992年7月27日 102(e)日期1992年7月27日PCT 1991年1月18日PCT PCT。 WO91 / 10732 PCT出版物 日期1991年7月25日本发明涉及一种表现出纤维素酶活性且可由芽孢杆菌属菌株NCIMB 40250或相关芽孢杆菌属菌种生产的酶。 菌株或所述纤维素酶的衍生物。 本发明还涉及一种酶,其包含衍生自内切葡聚糖酶的核心区域,所述内切葡聚糖酶与衍生自另一种纤维素酶的纤维素结合结构域结合,或包含衍生自与内切葡聚糖酶衍生的纤维素结合结构域的另一纤维素酶组成的核心区域。

    Alkaline cellulases
    56.
    发明授权
    Alkaline cellulases 失效
    碱性纤维素酶

    公开(公告)号:US5912157A

    公开(公告)日:1999-06-15

    申请号:US709979

    申请日:1996-09-09

    摘要: The present invention relates to cellulases derived from the strain Myceliophthora thermophila and variants thereof comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having a substrate binding cleft of a depth of at least 12 .ANG., exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.

    摘要翻译: 本发明涉及源自嗜热丝菌嗜热链霉菌菌株的纤维素酶及其变体,其包含核心和任选的由最多10个氨基酸组成的C-末端连接体,特别是具有至少12个神经元深度的底物结合裂缝的纤维素酶, 在碱性pH范围内表现出增强的酶活性,同时在纤维素基质上施加中等的纤维素分解作用,并且例如可用于洗涤剂组合物,特别是用于去除污垢或着色澄清或防止背染; 织物柔软剂; 用于纺织品的生物抛光; 用于纸浆排水改良; 用于脱墨的旧纸; 用于植物降解。

    Cellulase variants and detergent compositions containing cellulase
variants
    57.
    发明授权
    Cellulase variants and detergent compositions containing cellulase variants 失效
    含有纤维素酶变体的纤维素酶变体和洗涤剂组合物

    公开(公告)号:US5792641A

    公开(公告)日:1998-08-11

    申请号:US411777

    申请日:1995-05-05

    摘要: A cellulase variant of a parent cellulase, e.g. a cellulase classified in family 45 such as a Humicola insolens 43 kD endoglucanase, comprising a cellulose binding domain (CBD), a catalytically active domain (CAD) and a region linking the cellulose binding domain and catalytically active domain (the linking region), wherein one more amino acid residues of the CBD, CAD or linking region is deleted or substituted by one or more amino acid residues and/or one or more amino acids are added to the linking region and/or another CBD is added at the opposite end of the catalytically active domain, has improved properties as regards e.g. alkaline activity, compatibility with detergent composition ingredients, particulate soil removal, color clarification, defuzzing, depilling, harshness reduction, and sensitivity to anionic surfactants and peroxidase bleaching systems and is useful e.g. in detergent compositions, for textile treatment, in paper pulp processing, for animal feed and for stone washing of jeans.

    摘要翻译: PCT No.PCT / DK93 / 00327 Sec。 371日期:1995年5月5日 102(e)日期1995年5月5日PCT提交1993年10月6日PCT公布。 出版物WO94 / 07998 日期1994年4月14日一种亲本纤维素酶的纤维素酶变体,例如 例如分类在家族45中的纤维素酶,例如包含纤维素结合结构域(CBD),催化活性结构域(CAD)和连接纤维素结合结构域和催化活性结构域(连接区域)的区域的腐质霉43kD内切葡聚糖酶,其中 CBD,CAD或连接区的一个氨基酸残基被一个或多个氨基酸残基缺失或取代,和/或一个或多个氨基酸被添加到连接区和/或另一个CBD被加入到 催化活性域,具有改进的性能,例如 碱性活性,与洗涤剂组合物成分的相容性,颗粒污垢去除,颜色澄清,去污,去毛刺,粗糙度降低以及对阴离子表面活性剂和过氧化物酶漂白体系的敏感性。 在洗涤剂组合物中,用于纺织品处理,纸浆加工,动物饲料和牛仔裤的石洗。