-
公开(公告)号:US08445242B2
公开(公告)日:2013-05-21
申请号:US13070130
申请日:2011-03-23
CPC分类号: C11D3/38636 , C11D3/3945 , C12N9/18 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US20130102664A1
公开(公告)日:2013-04-25
申请号:US13657575
申请日:2012-10-22
IPC分类号: C12N9/18 , C12N15/63 , C12N1/15 , C12N1/19 , C12N1/21 , D21C9/16 , C12M1/40 , A01N37/16 , C11D3/395 , D06L3/02 , A01P1/00 , C12N15/55 , C12P7/40
CPC分类号: C12N9/18 , C11D3/386 , C11D3/38654 , C11D3/38681 , C11D3/3945 , C12P7/40 , C12Y301/01072 , D06L1/04 , D06L4/15 , D06M16/00 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US08394616B2
公开(公告)日:2013-03-12
申请号:US13328350
申请日:2011-12-16
CPC分类号: C11D3/38636 , C11D3/2093 , C11D3/3942 , C11D3/3947 , C12N9/18 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US08389259B2
公开(公告)日:2013-03-05
申请号:US13328381
申请日:2011-12-16
CPC分类号: A61L2/186 , A61L2/22 , A61L2/23 , A61L2202/24 , C12N9/18 , C12Y301/01072
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US08389257B2
公开(公告)日:2013-03-05
申请号:US13328327
申请日:2011-12-16
CPC分类号: C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US08389256B2
公开(公告)日:2013-03-05
申请号:US13328305
申请日:2011-12-16
CPC分类号: C11D3/3945 , C12N9/18 , C12P7/40 , C12P7/54 , C12Y301/01072 , D21C5/005 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US20120156156A1
公开(公告)日:2012-06-21
申请号:US13328327
申请日:2011-12-16
IPC分类号: C12P7/40 , C12N15/63 , C12N1/15 , C12N1/19 , C12N1/21 , D21C9/16 , A01N37/16 , A01P1/00 , A61L2/16 , C12M1/40 , D06L3/02 , C12N15/55 , C12N9/18
CPC分类号: C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US20110250673A1
公开(公告)日:2011-10-13
申请号:US13070118
申请日:2011-03-23
IPC分类号: C12N9/18
CPC分类号: C12N9/18 , C12Y301/01072
摘要: A two-stage heat treatment process is provided to improve the processability of recombinant microbial biomass comprising an enzyme having perhydrolytic activity. More specifically, a process is provided to treat the recombinant microbial biomass comprising a Thermotoga sp. acetyl xylan esterase having perhydrolytic activity such that microfiltration may be used to partially-purify and/or concentrate protein preparations. The acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
摘要翻译: 提供两阶段热处理方法以改善包含具有过水解活性的酶的重组微生物生物质的加工性能。 更具体地,提供了一种处理包含Thermotoga sp。的重组微生物生物质的方法。 具有过水解活性的乙酰木聚糖酯酶,使得微量过滤可用于部分纯化和/或浓缩蛋白质制剂。 乙酰木聚糖酯酶可用于制备适用于各种应用的过氧羧酸,例如清洁,消毒,消毒,漂白,木浆加工和纸浆加工应用。
-
公开(公告)号:US20110152370A1
公开(公告)日:2011-06-23
申请号:US13036752
申请日:2011-02-28
CPC分类号: C12N9/18 , A61L2/186 , C12P7/40 , C12Y301/01072
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号:US07932072B1
公开(公告)日:2011-04-26
申请号:US12632425
申请日:2009-12-07
CPC分类号: C12N9/18 , A61L2/186 , C12P7/40 , C12Y301/01072
摘要: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
-
-
-
-
-
-
-
-