利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

275 条记录 (耗时 0.069 秒)

    Small volume in vitro analyte sensor
    64.
    发明授权
    Small volume in vitro analyte sensor 有权
    体积小体积分析物传感器

    公开(公告)号：US08142643B2

    公开(公告)日：2012-03-27

    申请号：US12568858

    申请日：2009-09-29

    申请人： Adam Heller ,  Benjamin J. Feldman ,  James Say ,  Mark S. Vreeke

    发明人： Adam Heller ,  Benjamin J. Feldman ,  James Say ,  Mark S. Vreeke

    IPC分类号： G01N27/327

    CPC分类号： G01N27/3272 ,  A61B5/1411 ,  A61B5/14532 ,  A61B5/14546 ,  A61B5/1486 ,  A61B5/14865 ,  A61B5/1495 ,  A61B5/150022 ,  A61B5/150358 ,  A61B5/150412 ,  A61B5/150503 ,  A61B5/15105 ,  A61B5/15113 ,  A61B5/15144 ,  A61B5/157 ,  A61B2560/0223 ,  A61B2562/0295 ,  C12Q1/001 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3273 ,  G01N33/5438 ,  G01N2333/904

    摘要： A sensor designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator. The redox mediator acts as an electron transfer agent between the analyte and the electrode. In addition, a second electron transfer agent, such as an enzyme, can be added to facilitate the electrooxidation or electroreduction of the analyte. The redox mediator is typically a redox compound bound to a polymer. The preferred redox mediators are air-oxidizable.The amount of analyte can be determined by coulometry. One particular coulometric technique includes the measurement of the current between the working electrode and a counter or reference electrode at two or more times. The charge passed by this current to or from the analyte is correlated with the amount of analyte in the sample. Other electrochemical detection methods, such as amperometric, voltammetric, and potentiometric techniques, can also be used.The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is provided as a second electron transfer agent.

    摘要翻译： 设计用于确定体积小于约1μL的样品中分析物的量和浓度的传感器。 该传感器具有涂覆有不可浸出的氧化还原介体的工作电极。 氧化还原介体作为分析物和电极之间的电子转移剂。 此外，可以加入第二电子转移剂，例如酶，以促进分析物的电氧化或电还原。 氧化还原介体通常是与聚合物结合的氧化还原化合物。 优选的氧化还原介体是可空气氧化的。 分析物的量可以通过库仑法测定。 一种特定的库仑技术包括两次或更多次测量工作电极与计数器或参考电极之间的电流。 通过该电流流向或来自分析物的电荷与样品中分析物的量相关。 还可以使用其他电化学检测方法，例如电流，伏安和电位技术。 本发明可用于测定生物液体如血液或血清中生物分子如葡萄糖或乳酸盐的浓度。 提供能够催化生物分子的电氧化或电还原的酶作为第二电子转移剂。