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    Mobilization of viral genomes from T-DNA using site-specific recombination systems
    61.
    发明授权
    Mobilization of viral genomes from T-DNA using site-specific recombination systems 有权
    使用位点特异性重组系统动员来自T-DNA的病毒基因组

    公开(公告)号：US07807868B2

    公开(公告)日：2010-10-05

    申请号：US12061315

    申请日：2008-04-02

    申请人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    发明人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    IPC分类号： C12N15/82 ,  C12N15/90 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/90

    摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.

    摘要翻译： 本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。 本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。 感兴趣的DNA还可以含有重组酶的不相同的靶位点。 用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。 位点特异性重组酶在植物细胞中的表达导致切除病毒复制子和感兴趣的相关DNA。 然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。

    MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS
    63.
    发明申请
    MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS 有权
    使用现场特异性重组系统从T-DNA中动员病毒基因

    公开(公告)号：US20080282426A1

    公开(公告)日：2008-11-13

    申请号：US12061315

    申请日：2008-04-02

    申请人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    发明人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/90

    摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.

    摘要翻译： 本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。 本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。 感兴趣的DNA还可以含有重组酶的不相同的靶位点。 用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。 位点特异性重组酶在植物细胞中的表达导致病毒复制子和感兴趣的相关DNA的切除。 然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。 本发明的组合物包含核酸，例如含有侧翼为用于位点特异性重组酶的直接重复靶位点的病毒DNA的T-DNA。 本发明的核酸可以另外含有编码针对病毒基因组侧翼的靶位点的同源位点特异性重组酶的表达盒。 本发明的组合物还包含含有本发明核酸的农杆菌。 本发明的组合物和方法用于增加农业感染的效率，为临时表达提供高拷贝数的目的DNA，或用于整合到植物染色体中，以及简化用于农业感染和转化的载体的构建和稳定维持 。

    Transformation-enhancing compositions and methods of use
    65.
    发明授权
    Transformation-enhancing compositions and methods of use 有权
    转化增强组合物和使用方法

    公开(公告)号：US06444470B1

    公开(公告)日：2002-09-03

    申请号：US09425510

    申请日：1999-10-22

    申请人： Margit C. Ross ,  Laura A. Church ,  William J. Gordon-Kamm

    发明人： Margit C. Ross ,  Laura A. Church ,  William J. Gordon-Kamm

    IPC分类号： C12N1582

    CPC分类号： C12N15/8201

    摘要： The invention relates to compositions and methods and for genetically transforming Zea mays plants. Compositions comprising transformation-enhancing agents and methods of use are provided. The methods involve administering an effective amount of a transformation-enhancing agent to a Zea mays plant. The compositions and methods of the invention find use in improving transformation efficiency and increasing the embryogenic response of callus.

    摘要翻译： 本发明涉及组合物和方法以及用于转基因玉米植物。 提供了包含转化增强剂和使用方法的组合物。 所述方法包括向玉蜀黍植物施用有效量的转化增强剂。 本发明的组合物和方法用于提高转化效率并增加愈伤组织的胚胎发生反应。

    Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
    66.
    发明授权
    Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs 有权
    受精卵，卵或精子细胞的转化以及从分离的胚囊恢复转化的植物

    公开(公告)号：US06300543B1

    公开(公告)日：2001-10-09

    申请号：US09147489

    申请日：1999-05-12

    申请人： David D. Cass ,  Locksley E. McGann ,  Guichang Zhang ,  John D. Laurie ,  Jerome P. Ranch ,  William J. Gordon-Kamm

    发明人： David D. Cass ,  Locksley E. McGann ,  Guichang Zhang ,  John D. Laurie ,  Jerome P. Ranch ,  William J. Gordon-Kamm

    IPC分类号： C12N1582

    CPC分类号： C12N15/8201

    摘要： Methodology is provided for the production of uniformly transformed plants capable of transmitting a foreign gene to progeny by sexual reproduction. A foreign gene is introduced into the zygote in an isolated embryo sac and a transformed plant is recovered. Alternatively, a foreign gene is introduced into an egg cell in an isolated embryo sac, the egg cell is fertilized with an isolated sperm cell and a transformed plant is recovered. Sperm cells may be transformed with a foreign gene, an egg cell in an isolated embryo sac is fertilized with the transformed sperm cells, or nuclei isolated from the transformed sperm cells, and a transgenic plant is recovered. Another method for the production of transgenic plants is transformation of an embryo in an isolated embryo sac. The transgenic plant produced by any one of these methods is homogeneously transformed and capable of transmitting the foreign gene to progeny by sexual reproduction.

    摘要翻译： 提供方法用于生产能够通过有性繁殖将外来基因传播给后代的均匀转化植物。 将外源基因导入分离的胚囊中的受精卵并回收转化的植物。 或者，外源基因被引入到分离的胚囊中的卵细胞中，卵细胞用分离的精子细胞受精并回收转化的植物。 精子细胞可以用外源基因转化，分离的胚囊中的卵细胞用转化的精子细胞受精，或从转化的精子细胞中分离出核，并回收转基因植物。 用于生产转基因植物的另一种方法是在分离的胚囊中转化胚胎。 通过这些方法中的任何一种产生的转基因植物被均匀转化，并且能够通过有性繁殖将外源基因转移到后代。

    Method for the integration of foreign DNA into eukaryotic genomes
    67.
    发明授权
    Method for the integration of foreign DNA into eukaryotic genomes 有权
    将外源DNA整合到真核生物基因组中的方法

    公开(公告)号：US06262341B1

    公开(公告)日：2001-07-17

    申请号：US09193503

    申请日：1998-11-17

    申请人： Christopher L. Baszczynski ,  Leszek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan ,  Argula Gururaj Rao ,  Laura A. Tagliani

    发明人： Christopher L. Baszczynski ,  Leszek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan ,  Argula Gururaj Rao ,  Laura A. Tagliani

    IPC分类号： C12N504

    CPC分类号： C12N15/90 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8213

    摘要： Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells. The methods result in the efficient targeted genomic integration of DNA by site-specific recombination.

    摘要翻译： 提供了将目的DNA引入基因组靶位点的组合物和方法。 特别地，所述方法和组合物包括使用两个位点特异性重组酶的靶位点的组合和具有双靶点位点特异性的嵌合重组酶的表达。 因此，组合物包含对多个靶位点具有特异性的新的位点特异性重组酶，以及编码这些重组酶或靶位点的核苷酸序列和表达盒。 所述方法包括用具有相应靶位点侧翼的感兴趣的DNA转化具有新重组酶靶位点的真核细胞。 重组酶的表达导致感兴趣的DNA整合到细胞的基因组中。 本发明的组合物和方法用于构建稳定转化的真核细胞，特别是植物细胞。 该方法通过位点特异性重组导致DNA的有效靶向基因组整合。

    SELECTIVE ABLATION OF DIPLOID EMBRYOS
    69.
    发明申请
    SELECTIVE ABLATION OF DIPLOID EMBRYOS 审中-公开
    胆固醇的选择性消除

    公开(公告)号：US20120311736A1

    公开(公告)日：2012-12-06

    申请号：US13586893

    申请日：2012-08-16

    申请人： Mark E. Williams ,  William J. Gordon-Kamm

    发明人： Mark E. Williams ,  William J. Gordon-Kamm

    IPC分类号： A01H1/04 ,  A01H1/02 ,  A01H5/00

    CPC分类号： C12N15/8263 ,  A01H1/08 ,  C12N15/8217 ,  C12N15/8287

    摘要： Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.

    摘要翻译： 公开了选择单倍体胚胎的方法。 提供了在植物上产生单倍体胚胎和不可行的二倍体胚胎的方法。 提供了用于选择由单倍体诱导物玉米品系生产的单倍体胚胎的方法。 公开了生产改良的玉米单倍体诱导物系的方法。 公开了包含导致烧蚀或异常二倍体胚的转基因的玉米单倍体诱导物系。

    Methods and compositions for in planta production of inverted repeats
    70.
    发明授权
    Methods and compositions for in planta production of inverted repeats 失效
    在反向重复的植物生产中的方法和组合物

    公开(公告)号：US08058509B2

    公开(公告)日：2011-11-15

    申请号：US11611661

    申请日：2006-12-15

    申请人： Kimberly Glassman ,  William J. Gordon-Kamm ,  Rudolf Jung

    发明人： Kimberly Glassman ,  William J. Gordon-Kamm ,  Rudolf Jung

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87

    CPC分类号： C12N15/8213 ,  C12N15/8216

    摘要： Methods and compositions are provided which allow for the production of an inverted repeat in a plant or plant part, which, when transcribed as an RNA can be used in some examples to decrease expression of a target polynucleotide of interest. The methods and composition provide precursor inverted repeat cassettes that are not capable of producing a hairpin RNA polynucleotide, and plants and plant parts comprising such precursor inverted repeat cassettes. The precursor inverted repeat cassettes are altered in planta by using a recombination system to produce a inverted repeat. Plants, plant parts, and seeds comprising the various components are also provided.

    摘要翻译： 提供了允许在植物或植物部分中产生反向重复的方法和组合物，其在转录为RNA时可用于一些实例中以减少目的目的多核苷酸的表达。 方法和组合物提供不能产生发夹RNA多核苷酸的前体反向重复盒，以及包含这种前体反向重复盒的植物和植物部分。 通过使用重组系统产生反向重复，在植物中改变前体反向重复盒。 还提供了包括各种组分的植物，植物部分和种子。