公开(公告)号：US5969213A

公开(公告)日：1999-10-19

申请号：US446930

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  May L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  May L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to stable and genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the invention include, but are not limited to, wheat, barley and oats. The invention also pertains to transgenic cereal plants which further comprise screenable markers and transgenic seed obtained from said plants.

摘要翻译： 本发明涉及除了玉米或水稻之外的稳定和遗传转化的谷类植物，其包含表达膦丝菌素乙酰转移酶的外源DNA，以在所述谷类植物中赋予对于草丁膦的抗性。 本发明范围内的谷类植物包括但不限于小麦，大麦和燕麦。 本发明还涉及进一步包含从所述植物获得的可筛选标记和转基因种子的转基因谷物植物。

公开(公告)号：US5489520A

公开(公告)日：1996-02-06

申请号：US233067

申请日：1994-04-26

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US07705215B1

公开(公告)日：2010-04-27

申请号：US08113561

申请日：1993-08-25

申请人： Thomas R. Adams ,  Paul C. Anderson ,  Sheryl A. Chambers ,  Paul S. Chomet ,  Richard J. Daines ,  Christopher E. Flick ,  Kimberly Glassman ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Lucille B. Laccetti ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary Lou Mangano ,  Michael T. Mann ,  James V. O'Brien ,  Emil M. Orozco ,  Peter Orr ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Michael A. Stephens ,  Clayton S. Vetsch ,  David A. Walters ,  Donald S. Walters ,  Nancy G. Willetts ,  Susan J. Zachwieja

发明人： Thomas R. Adams ,  Paul C. Anderson ,  Sheryl A. Chambers ,  Paul S. Chomet ,  Richard J. Daines ,  Christopher E. Flick ,  Kimberly Glassman ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Lucille B. Laccetti ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary Lou Mangano ,  Michael T. Mann ,  James V. O'Brien ,  Emil M. Orozco ,  Peter Orr ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Michael A. Stephens ,  Clayton S. Vetsch ,  David A. Walters ,  Donald S. Walters ,  Nancy G. Willetts ,  Susan J. Zachwieja

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10 ,  C12N15/53 ,  C12P7/64

CPC分类号： C12N15/8241 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N5/0025 ,  C12N5/04 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/87

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US5919675A

公开(公告)日：1999-07-06

申请号：US446933

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

摘要翻译： 本发明涉及一种用于生产稳定的转基因玉米植物的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述植物中的次氯氰菊酯抗性。 该方法还包括用第二基因转化所述玉米植物，并且还进一步其中所述第二基因赋予昆虫抗性，例如苏云金芽孢杆菌毒素基因的表达。 实现稳定转化的方法包括组织培养方法和培养基，用所述DNA轰击受体细胞的方法，以及从转化细胞再生可育植物的方法。 本发明还涉及用非转基因植物或转基因植物育种所述转基因玉米植物的方法，以及获得可育转基因植物和种子。

公开(公告)号：US5874265A

公开(公告)日：1999-02-23

申请号：US446931

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a method for the production of stable, genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the method include, but are not limited to, wheat, barley and oats. The invention also pertains to a method of breeding said transgenic cereal plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

摘要翻译： 本发明涉及一种生产稳定的基因转化谷物植物（玉米或水稻以外）的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述谷类植物中的次氯氰菊酯抗性。 在该方法范围内的谷类植物包括但不限于小麦，大麦和燕麦。 本发明还涉及用非转基因植物或转基因植物育种所述转基因谷物植物的方法，以及获得可育转基因植物和种子。

公开(公告)号：US5550318A

公开(公告)日：1996-08-27

申请号：US565844

申请日：1990-08-09

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

公开(公告)号：US06235529B1

公开(公告)日：2001-05-22

申请号：US08845939

申请日：1997-04-29

申请人： Peggy G. Lemaux ,  Myeong-Je Cho

发明人： Peggy G. Lemaux ,  Myeong-Je Cho

IPC分类号： C12N504

CPC分类号： A01H4/005

摘要： Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.

摘要翻译： 公开了用于从胚性愈伤组织转化和再生植物的改进的组合物和方法，其包括例如：在愈伤组织诱导后使用中间培养培养基以增加转化细胞的再生能力; 选择早期阶段的昏暗条件; 使用绿色愈伤组织作为微粒轰击的目标; 和具有优化水平的植物激素和铜浓度的培养基。

公开(公告)号：US07429691B2

公开(公告)日：2008-09-30

申请号：US10526663

申请日：2003-09-03

申请人： Shibo Zhang ,  Rosalind Carrier ,  Peggy G. Lemaux

发明人： Shibo Zhang ,  Rosalind Carrier ,  Peggy G. Lemaux

IPC分类号： C12N15/82

CPC分类号： A01H4/005

摘要： Methods for transforming plants, particularly commercially important elite maize inbreds, are provided. The methods involve transformation of meristematic organogenic tissue or immature embryos, and include the use of defined plant growth media. The methods disclosed provide more stable transgenic plants, and permit the transformation of varieties of cereals that are not amenable to transformation by conventional approaches.

摘要翻译： 提供了转化植物，特别是商业上重要的精英玉米近交系的方法。 该方法涉及分生组织或未成熟胚的转化，并包括使用定义的植物生长培养基。 所公开的方法提供更稳定的转基因植物，并允许通过常规方法转化不适合转化的谷物品种。

公开(公告)号：US06342380B1

公开(公告)日：2002-01-29

申请号：US09601091

申请日：2000-09-19

申请人： Sheila M. Colby ,  John E. Crock ,  Peggy G. Lemaux ,  Rodney B. Croteau

发明人： Sheila M. Colby ,  John E. Crock ,  Peggy G. Lemaux ,  Rodney B. Croteau

IPC分类号： C12N900

CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12N15/8279 ,  C12N15/8286 ,  C12Y402/03009 ,  Y02A40/162

摘要： Germacrene C synthase genes from Lycopersicon esculentum have been cloned and sequenced. Transgenic expression of germacrene C synthase in plants can result in beneficial and useful characteristics such as increased host resistance to pathogens and herbivores and altered flavor and odor profiles.

摘要翻译： 已经克隆并测序了来自番茄的Germacrene C合酶基因。 植物中锗菌C合酶的转基因表达可以产生有益和有用的特征，例如增加宿主对病原体和食草动物的抗性以及改变的风味和气味特征。

公开(公告)号：US06784346B1

公开(公告)日：2004-08-31

申请号：US09538864

申请日：2000-03-29

申请人： Myeong-Je Cho ,  Peggy G. Lemaux ,  Bob B. Buchanan ,  Joshua Wong ,  Corina Marx

发明人： Myeong-Je Cho ,  Peggy G. Lemaux ,  Bob B. Buchanan ,  Joshua Wong ,  Corina Marx

IPC分类号： A01H500

CPC分类号： C12N15/8261 ,  A21D13/064 ,  A23J1/12 ,  A23K10/30 ,  A23L33/105 ,  A23V2002/00 ,  C07K14/415 ,  C12N9/0036 ,  C12N15/8242 ,  C12N15/8245 ,  C12N15/8251 ,  Y02A40/146 ,  A23V2300/21

摘要： Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.

摘要翻译： 本文提供了组合物和使用方法以制备和使用具有增值性状的转基因植物。