公开(公告)号：US20180105822A1

公开(公告)日：2018-04-19

申请号：US15798319

申请日：2017-10-30

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski

IPC: C12N15/82 ,  C12N15/113

Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

公开(公告)号：US20180002690A1

公开(公告)日：2018-01-04

申请号：US15476701

申请日：2017-03-31

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/8222

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US09771595B2

公开(公告)日：2017-09-26

申请号：US14625566

申请日：2015-02-18

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski

IPC: C12N15/82 ,  C12N15/113 ,  C12N15/11 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415 ,  C12N15/11 ,  C12N15/113 ,  C12N15/8201 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8274 ,  C12N15/8279

Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

公开(公告)号：US20170044568A1

公开(公告)日：2017-02-16

申请号：US15221544

申请日：2016-07-27

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Stanislaw Flasinski ,  Yong Yin

IPC: C12N15/82 ,  A01N37/18 ,  C07K14/325 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C12Q1/6897 ,  Y02A40/162

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed, and includes, but is not limited to, TIC5290. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC5290 pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC5290 pesticidal protein of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the TIC5290 pesticidal protein are also provided.

Abstract translation: 公开了对鞘翅目，鳞翅目和半翅目害虫物种具有毒性活性的杀虫蛋白质类，包括但不限于TIC5290。 提供含有编码TIC5290杀虫蛋白的重组核酸序列的DNA构建体。 提供了转基因植物，植物细胞，种子和对鳞翅目，鞘翅目和半翅目的侵染的植物部分，其含有编码本发明的TIC5290杀虫蛋白的重组核酸序列。 还提供了在生物样品中检测本发明的重组核酸序列或蛋白质的存在的方法，以及使用TIC5290杀虫蛋白质来控制鞘翅目，鳞翅目和半翅目种类害虫的方法。

公开(公告)号：US20170029834A1

公开(公告)日：2017-02-02

申请号：US15222783

申请日：2016-07-28

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Shirley Xiaoli Guo ,  Wei Wu

IPC: C12N15/82 ,  C12N7/00

CPC classification number: C12N15/8216 ,  C12N15/8227 ,  C12N2730/00043

Abstract: The present invention provides regulatory polynucleotide molecules isolated from plant proline rich protein genes and linked to a viral enhancer molecule. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the regulatory polynucleotide sequences, and methods for preparing and using the same.

Abstract translation: 本发明提供从植物富含脯氨酸的蛋白质基因分离并连接到病毒增强子分子的调节性多核苷酸分子。 本发明进一步公开了组合物，多核苷酸构建体，转化的宿主细胞，含有调控多核苷酸序列的转基因植物和种子，以及制备和使用其的方法。

公开(公告)号：US20160289693A1

公开(公告)日：2016-10-06

申请号：US15179635

申请日：2016-06-10

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski

IPC: C12N15/82

Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

公开(公告)号：US09441240B2

公开(公告)日：2016-09-13

申请号：US13890027

申请日：2013-05-08

Applicant: Monsanto Technology LLC

Inventor： Wen C. Burns ,  Catherine A. Chay ,  Cheryl L. Cloninger ,  Mingqi Deng ,  Stanislaw Flasinski ,  Kunsheng Wu

IPC: A01H5/00 ,  C12N15/11 ,  C12N15/52 ,  C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8286 ,  A01N57/20 ,  C12N15/8218 ,  C12N15/8275 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  Y02A40/162

Abstract: The invention provides corn event MON 87411, and plants, plant cells, seeds, plant parts, and commodity products comprising event MON 87411. The invention also provides polynucleotides specific for event MON 87411 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87411. The invention also provides methods related to event MON 87411.

Abstract translation: 本发明提供了玉米事件MON 87411以及包含事件MON 87411的植物，植物细胞，种子，植物部分和商品。本发明还提供了针对事件MON 87411和植物，植物细胞，种子，植物部分和商品特异性的多核苷酸 包含特定于事件MON 87411的多核苷酸的产物。本发明还提供了与事件MON 87411相关的方法。

公开(公告)号：US20160108426A1

公开(公告)日：2016-04-21

申请号：US14884432

申请日：2015-10-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  A01N63/02 ,  A01N47/08 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N47/08 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

Abstract translation: 提供了与天然存在的Cry1Da蛋白毒素相比表现出改进的鳞翅目杀虫活性和增强的鳞翅目光谱的工程化Cry1Da氨基酸序列。 还提供了用于在植物中表达改良蛋白质的多核苷酸序列。 具体实施方案提供了含有昆虫抑制量的工程改造的蛋白质的组合物，以及含有编码一种或多种改进的工程化蛋白质的多核苷酸构建体的重组植物，植物部分和种子。

公开(公告)号：US20140283201A1

公开(公告)日：2014-09-18

申请号：US14207302

申请日：2014-03-12

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Jun Zhang ,  Suling Zhao

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C07K14/415 ,  C12N15/8212 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8241 ,  C12N15/8274 ,  C12N15/8279

Abstract: The invention provides recombinant DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising a recombinant DNA molecule comprising a DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of their use.

Abstract translation: 本发明提供了可用于调节植物中基因表达的重组DNA分子和构建体及其核苷酸序列。 本发明还提供转基因植物，植物细胞，植物部分和种子，其包含重组DNA分子，其包含与异源可转录的DNA分子可操作地连接的DNA分子，以及它们的使用方法。

公开(公告)号：US20130198886A1

公开(公告)日：2013-08-01

申请号：US13774794

申请日：2013-02-22

Applicant: Monsanto Technology LLC

Inventor： Paul C.C. Feng ,  Marianne Malven ,  Stanislaw Flasinski

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8274 ,  C07K14/415 ,  C07K2319/08 ,  C12N9/0069 ,  C12N15/8221

Abstract: The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.