公开(公告)号：US20110312816A1

公开(公告)日：2011-12-22

申请号：US13150245

申请日：2011-06-01

申请人： Kia Silverbrook ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Mehdi Azimi

IPC分类号： C40B60/12 ,  G01N21/76

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, an excitation LED for generating the excitation light, the LED positioned in the outer casing for simultaneously illuminating all the probes.

摘要翻译： 一种测试模块，其具有尺寸适于手持便携性的外壳，外壳具有用于接收含有靶核酸序列的生物样品的入口，用于杂交的外壳中的探针具有用于与靶核酸杂交的核酸序列 酸序列以形成探针 - 靶杂交体，所述探针 - 靶杂交体被配置为响应于激发光产生荧光信号，以及用于产生激发光的激发LED，所述LED位于外壳中以同时发光 所有的探针。

公开(公告)号：US20110312797A1

公开(公告)日：2011-12-22

申请号：US13150207

申请日：2011-06-01

申请人： Alireza Moini ,  Mehdi Azimi ,  Kia Silverbrook

发明人： Alireza Moini ,  Mehdi Azimi ,  Kia Silverbrook

IPC分类号： C40B60/12

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a casing configured for hand held portability, the casing having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light source for illuminating the array of probes, and a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences.

摘要翻译： 一种用于检测探针与靶核酸序列的杂交的测试模块，所述测试模块具有配置为手持便携性的壳体，所述壳体具有用于接收具有靶核酸序列的生物样品的容器，用于与靶核酸序列杂交的探针阵列 靶核酸序列，用于照射探针阵列的激发光源和用于感测阵列中与靶核酸序列杂交的任何探针的荧光发射的光传感器。

公开(公告)号：US20110312796A1

公开(公告)日：2011-12-22

申请号：US13150206

申请日：2011-06-01

申请人： Darrin John Nagy ,  Mehdi Azimi ,  Kia Silverbrook

发明人： Darrin John Nagy ,  Mehdi Azimi ,  Kia Silverbrook

IPC分类号： C40B60/12 ,  C12M1/40

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module for analyzing a sample fluid and communicating data to a medical database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the medical database, and, a controller for operative control of the communication interface.

摘要翻译： 一种用于分析样本流体并将数据传送到医疗数据库的测试模块，测试模块具有用于接收样本的插座，用于处理和分析样本的功能部分，用于与医疗数据库通信的通信接口，以及控制器 用于操作控制通信接口。

公开(公告)号：US20110312795A1

公开(公告)日：2011-12-22

申请号：US13150204

申请日：2011-06-01

申请人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

IPC分类号： C40B60/12

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light emitting diode (LED) for illuminating the array of probes, CMOS circuitry having a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences such that the CMOS circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized, wherein, the array of probes and the CMOS circuitry are integrated on a lab-on-a-chip (LOC) device contained within the test module.

摘要翻译： 一种用于检测探针与靶核酸序列的杂交的测试模块，所述测试模块具有用于接收具有靶核酸序列的生物样品的容器，用于与靶核酸序列杂交的探针阵列，激发发光二极管 LED），用于照射探针阵列，CMOS电路具有用于感测阵列中与靶核酸序列杂交的任何探针的荧光发射的光电传感器，使得CMOS电路被配置为使用来自光传感器的输出 产生指示已经杂交的探针的信号，其中探针阵列和CMOS电路集成在包含在测试模块内的芯片上实验室（LOC）装置上。

公开(公告)号：US20110312783A1

公开(公告)日：2011-12-22

申请号：US13150174

申请日：2011-06-01

申请人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

IPC分类号： C40B60/12

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with a respective one of the target nucleic acid sequences to form a probe-target hybrid and emit a fluorescence signal in response to an excitation light, and, photosensors corresponding to each of the probes respectively, and, a control photosensor not corresponding to any of the probes such that the control photosensor does not sense the fluorescence signal of a corresponding probe-target hybrid during correct operation of the microfluidic device.

摘要翻译： 具有支撑底物的微流体装置，用于接收含有靶核酸序列的生物样品的入口，各自具有用于与靶核酸序列中的相应一个核酸序列杂交的核酸序列的探针，以形成探针 - 靶杂交体并发射 响应于激发光的荧光信号和分别对应于每个探针的光敏元件，以及不对应于任何探针的控制光电传感器，使得控制光电传感器不感测相应探针靶的荧光信号 在微流体装置的正确操作期间混合。

公开(公告)号：US20110312779A1

公开(公告)日：2011-12-22

申请号：US13150168

申请日：2011-06-01

申请人： Kia Silverbrook ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Mehdi Azimi

IPC分类号： C40B60/14 ,  C40B60/12

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of ejectors, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, and, bond-pads for electrically connecting the CMOS circuitry and an external microprocessor controller for operative control of the array of ejectors.

摘要翻译： 一种用于将探针非接触点样的表面的寡核苷酸点样装置，所述探针具有与生物样品中待鉴定的靶核酸序列互补的核酸序列，所述寡核苷酸点样装置具有支撑基底，排列阵列 具有用于喷射含有寡核苷酸探针的液滴的致动器，用于为每个液滴喷射致动器提供用于液滴喷射的驱动脉冲的CMOS电路，以及用于电连接CMOS电路的接合焊盘和用于操作控制的外部微处理器控制器 的喷射器阵列。

公开(公告)号：US20110312776A1

公开(公告)日：2011-12-22

申请号：US13150161

申请日：2011-06-01

申请人： Kia Silverbrook ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Mehdi Azimi

IPC分类号： C40B60/12 ,  G01N21/76 ,  C12M1/40

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a reporter with a fluorophore positioned for exposure to the excitation light simultaneously with the probes, wherein, the reporter always emits the fluorescence signal in response to the excitation light.

摘要翻译： 具有支撑底物的微流体装置，用于接收含有靶核酸序列的生物样品的入口，每个具有用于与靶核酸序列杂交以形成探针 - 靶杂交物的核酸序列的探针，荧光团和 猝灭剂被配置为使得荧光团响应于激发光发射荧光信号，并且当探针未杂交时淬灭剂淬灭荧光信号，但不能从探针 - 靶杂交体淬灭荧光信号，并且具有 定位用于与探针同时暴露于激发光的荧光团，其中报告人总是响应于激发光发出荧光信号。

公开(公告)号：US20110312772A1

公开(公告)日：2011-12-22

申请号：US13150138

申请日：2011-06-01

申请人： Mehdi Azimi ,  Kia Silverbrook ,  Alireza Moini

发明人： Mehdi Azimi ,  Kia Silverbrook ,  Alireza Moini

IPC分类号： C40B60/12 ,  C12M1/40

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A LOC device for amplifying nucleic acid sequences and detecting target nucleic acid sequences, the LOC device having a supporting substrate, restriction enzymes for digesting double stranded nucleic acid sequences at known restriction sites, linker primers for ligation to the restricted ends of the double stranded nucleic acid sequences, heaters for thermally cycling the nucleic acid sequences through a polymerase chain reaction (PCR) process, deoxyribonucleoside triphosphates (dNTPs) for extending the linker primers along the nucleic acid sequences, and, probes for hybridizing with target nucleic acid sequences.

摘要翻译： 用于扩增核酸序列和检测靶核酸序列的LOC装置，具有支持底物的LOC装置，用于在已知限制性位点消化双链核酸序列的限制酶，用于连接到双链核酸的限制性末端的连接引物 酸序列，用于通过聚合酶链反应（PCR）方法热循环核酸序列的加热器，用于沿着核酸序列延伸连接引物的脱氧核糖核苷三磷酸（dNTP）和用于与靶核酸序列杂交的探针。

公开(公告)号：US20110312768A1

公开(公告)日：2011-12-22

申请号：US13150130

申请日：2011-06-01

申请人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

IPC分类号： C40B60/12 ,  C12M1/34

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A LOC device having a supporting substrate, primer-linked, stem-and-loop probes, each with a nucleic acid sequence that matches one of a plurality of target nucleic acid sequences, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the fluid prior to hybridization with the FRET probes, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light.

摘要翻译： 一种LOC装置，其具有支持底物，引物连接的茎和环探针，每个具有与多个靶核酸序列中的一个相匹配的核酸序列，以及用于延伸靶核酸序列的引物以形成 互补序列，使得在使用期间，匹配靶核酸序列的核酸序列与互补序列退火以响应于激发光改变来自探针的荧光发射，用于扩增靶核酸的聚合酶链反应（PCR）部分 在与FRET探针杂交之前的流体中的酸序列以及支持衬底上的CMOS电路，CMOS电路具有对激发光的操作控制。

公开(公告)号：US20110312761A1

公开(公告)日：2011-12-22

申请号：US13150117

申请日：2011-06-01

申请人： Geoffrey Richard Facer ,  Kia Silverbrook ,  Mehdi Azimi

发明人： Geoffrey Richard Facer ,  Kia Silverbrook ,  Mehdi Azimi

IPC分类号： C40B60/12 ,  C12M1/40 ,  C12M1/00

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having a valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, the lysis section having a lysis heater for heating the fluid in the lysis section, wherein, the valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid.

摘要翻译： 一种用于裂解流体中的细胞的测试模块，所述测试模块具有外壳，其具有用于接收含有细胞的流体的容器，含有裂解试剂并具有阀的裂解剂储存器，以及与所述容器流体连通的裂解部分 为了在裂解细胞期间保持流体，裂解部分具有用于加热裂解部分中的流体的裂解加热器，其中，阀在裂解部分的上游并且被配置为当流体流入裂解部分时打开，从而添加 裂解试剂到液体。