公开(公告)号：US5843651A

公开(公告)日：1998-12-01

申请号：US434354

申请日：1995-05-02

申请人： Donald Irvine Stimpson ,  Julian Gordon ,  Joanell V. Hoijer

发明人： Donald Irvine Stimpson ,  Julian Gordon ,  Joanell V. Hoijer

IPC分类号： C12Q1/68 ,  G01N21/55 ,  G01N33/543 ,  G01N33/553 ,  C07H21/04 ,  C12Q1/70 ,  G01N25/20

CPC分类号： G01N21/47 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54373 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707

摘要： A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

摘要翻译： 波导结合测定方法包括检测引导到波导中的光的散射，散射是在ev逝波的穿透深度内特异地结合波导的散射标记的结果。 波导可以是透明塑料或玻璃，并且结合通常是通过寡核苷酸杂交或免疫捕获。 光散射标签包括胶体金属或非金属，包括金，硒和胶乳。 也可以使用由染料或浓缩颗粒组成的光吸收构件来增强信号。 可以直观地或通过视频成像来监视实时绑定和解离，例如使用CCD相机和帧抓取软件。 通过实时熔解曲线可以区分少至一个碱基的杂交错配。

公开(公告)号：US5645801A

公开(公告)日：1997-07-08

申请号：US516728

申请日：1995-08-18

申请人： Stanley R. Bouma ,  Ronald A. Coules ,  Julian Gordon ,  Eric B. Shain ,  Natalie A. Solomon ,  Peter Zaun

发明人： Stanley R. Bouma ,  Ronald A. Coules ,  Julian Gordon ,  Eric B. Shain ,  Natalie A. Solomon ,  Peter Zaun

IPC分类号： B01L7/00 ,  C12Q1/68 ,  C12M1/40 ,  C12P19/34

CPC分类号： B01L7/52 ,  Y10S435/81

摘要： Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.

摘要翻译： 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元结合操作的热循环装置。 将样品装载到装置的反应室中，然后与检测室密封地配合，以形成几乎不可逆地封闭的密封的反应/检测单元。 热循环装置的一个或多个加热元件对反应/检测装置施加所需的温度以放大样品中的靶核酸。 然后将反应混合物转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 试剂盒包括用于扩增的反应/检测单元和试剂。

公开(公告)号：US4956302A

公开(公告)日：1990-09-11

申请号：US355043

申请日：1989-05-15

申请人： Julian Gordon ,  Charles S. G. Pugh

发明人： Julian Gordon ,  Charles S. G. Pugh

IPC分类号： G01N33/558 ,  G01N37/00

CPC分类号： G01N33/558 ,  Y10T436/255

摘要： The present invention relates to improved specific binding assay devices comprising a chromatographic medium including a reaction site at which a specific binding reagent is immobilized, a sample application well located adjacent to the chromatographic medium and offset upstream from the reaction site, and a liquid absorption blotter offset downstream from the reaction site.

摘要翻译： 本发明涉及改进的特异性结合测定装置，其包括色谱介质，其包括固定特异性结合试剂的反应位点，位于邻近色谱介质并偏离反应位点上游的样品施加井和液体吸收吸墨纸 偏离反应位点的下游。