公开(公告)号：US20020182626A1

公开(公告)日：2002-12-05

申请号：US10098606

申请日：2002-03-15

发明人： Daniel Tuse

IPC分类号： C12Q001/68 ,  G01N033/53 ,  C12N015/87

CPC分类号： C12N15/1079

摘要： The present invention provides a method for functional genomic analysis and antigen prototyping by introducing episomal non-transforming non-viral vector-based cDNA or genomic libraries of donor organisms, pathogens, or cancer cells into host cells. The DNA libraries can be compacted without substantial aggregation to facilitate uptake by host cells. Antigen prototyping can be to identify antigens of pathogens that confer immuno-protection against the pathogens and antigens of cancer cells that confer immuno-protection against the cancer cells..

摘要翻译： 本发明提供了通过将供体生物体，病原体或癌细胞的附加型非转化非病毒载体的cDNA或基因组文库引入宿主细胞来进行功能基因组分析和抗原原型形成的方法。 可以压缩DNA文库而无需大量的聚集，以促进宿主细胞摄取。 抗原原型可以用于鉴定赋予针对癌细胞免疫保护的癌细胞的病原体和抗原的免疫保护的病原体抗原。

公开(公告)号：US20020174451A1

公开(公告)日：2002-11-21

申请号：US09437607

申请日：1999-11-10

发明人： RUFUS L. CHANEY ,  J. SCOTT ANGLE ,  YIN-MING LI ,  ALAN J.M. BAKER

IPC分类号： C12N015/87

CPC分类号： B09C1/105 ,  A01H5/00 ,  C22B3/18 ,  C22B23/00 ,  Y02P10/234

摘要： The invention relates to recovering metals, such as nickel and cobalt, by phytomining or phytoextracting soils rich in metals wherein the desired metal is selectively accumulated in hyperaccumulator plants by adjusting the soil pH. The metals are ultimately recovered from above-ground plant tissues at economically acceptable levels without further contaminating the metal-containing sites. The invention also relates to metal-hyperaccumulating plants.

摘要翻译： 本发明涉及通过植入或植入富含金属的土壤来回收金属，例如镍和钴，其中所需金属通过调节土壤pH值选择性地积累在超累积器植物中。 金属最终以经济可接受的水平从地上植物组织中回收，而不会进一步污染含金属的位点。 本发明还涉及金属超积累植物。

公开(公告)号：US20020173014A1

公开(公告)日：2002-11-21

申请号：US10060230

申请日：2002-02-01

发明人： Kalervo Hiltunen ,  Tuomo Glumoff

IPC分类号： C12P007/62 ,  C12N009/16 ,  C12N015/87

CPC分类号： C12N9/0006 ,  C12N9/88 ,  C12N15/8243 ,  C12P7/625

摘要： This invention relates to a method for controlling the production of cellular (3R)-hydroxyacyl CoA esters of predetermined length in a chosen host cell or organism. In particular, the invention relates to a modified gene encoding a multifunctional 2-enoyl-CoA hydratase 2/(3R)-hydroxyacyl CoA dehydrogenase enzyme type 2 protein and DNA constructs and host comprising the gene. The modification of the gene results in the enrichment of cellular (3R)-hydroxyacyl CoA esters of predetermined length, when the gene is introduced and expressed in a host cell oxidizing exogenous or endogenous null-fatty acids.

摘要翻译： 本发明涉及在所选宿主细胞或生物体中控制预定长度的细胞（3R） - 羟基酰基CoA酯的生产的方法。 特别地，本发明涉及编码多功能2-烯酰辅酶A水合酶2 /（3R） - 羟基酰基CoA脱氢酶2型蛋白质的修饰基因和包含该基因的DNA构建体和宿主。 当基因导入并在宿主细胞中表达，氧化外源性或内源性β-脂肪酸时，该基因的修饰导致具有预定长度的细胞（3R） - 羟基酰基CoA酯的富集。

公开(公告)号：US20020157135A1

公开(公告)日：2002-10-24

申请号：US09899954

申请日：2001-07-06

申请人： The University of Queensland

发明人： Ian Hector Frazer ,  Jose Ramon Botella Mesa ,  Jian Zhou ,  Xiao Yi Sun

IPC分类号： A01H001/00 ,  C12N015/87

CPC分类号： C12N15/8241 ,  C12N15/8216 ,  C12N15/8222

摘要： A method is disclosed for constructing a synthetic polynucleotide from which a protein is selectively expressible in a target cell of a plant, relative to another cell of the plant. The method comprises selecting a first codon of a parent polynucleotide for replacement with a synonymous codon which has a higher translational efficiency in the target cell than in said other cell, and replacing said first codon with said synonymous codon to form said synthetic polynucleotide.

摘要翻译： 公开了一种用于构建合成多核苷酸的方法，其中相对于植物的另一个细胞，蛋白质在植物的靶细胞中可选择性地表达。 所述方法包括选择母体多核苷酸的第一个密码子替换，所述同义密码子在靶细胞中具有比所述其他细胞更高的翻译效率，并用所述同义密码子替换所述第一密码子以形成所述合成多核苷酸。

公开(公告)号：US20020157128A1

公开(公告)日：2002-10-24

申请号：US09029941

申请日：1998-04-16

发明人： JAMES ANDREW CHARLES SMITH ,  UTE KRAMER ,  ALAN JOHN MARTIN BAKER

IPC分类号： C12N015/87

CPC分类号： B09C1/105 ,  C12N15/52 ,  C12N15/8242 ,  C12N15/8251 ,  C12N15/8259

摘要： Materials and methods for removing metal pollutants from metal-containing substrates, particularly soil environments, using plants modified so as to contain an increased concentration of the amino acid histidine responsible for metal uptake and tolerance.

摘要翻译： 使用修饰以包含负责金属吸收和耐受性的氨基酸组氨酸浓度增加的植物从金属底物，特别是土壤环境中去除金属污染物的材料和方法。

公开(公告)号：US20020156042A1

公开(公告)日：2002-10-24

申请号：US10022127

申请日：2001-10-30

发明人： Rekha G. Panchal ,  Charles J. Link JR.

IPC分类号： A61K048/00 ,  C07H021/04 ,  C12N015/87

CPC分类号： H04B10/2504 ,  A61K48/00 ,  C12N9/22 ,  C12N15/11 ,  C12N2799/028

摘要： Novel synthetic suppressor tRNA have been provided which provide read-through of internal nonsense mutations, or which can site-specifically alter translation of transcribed sequences. Uses of the same are also provided in genetic engineering protocols including gene therapy treatment of diseases such as Xeroderma pigmentosum.

摘要翻译： 已经提供了新的合成抑制基因tRNA，其提供了内部无义突变的阅读，或者可以特异性地改变转录序列的翻译。 在遗传工程方案中还提供了其用途，包括染色剂如色素木病等基因治疗治疗。

公开(公告)号：US20020155609A1

公开(公告)日：2002-10-24

申请号：US10112036

申请日：2002-04-01

申请人： Greenville Hospital System

发明人： Thomas E. Wagner ,  Xianzhang Yu

IPC分类号： C12N015/86 ,  C12N015/87

CPC分类号： C12N15/87 ,  A61K39/00 ,  A61K39/0005 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/55555

摘要： The inventive vector specifically directs entry into a cell of monocytic origin. The vector is composed of a nucleic acid component, a lysosome evading component and a particle that can be phagocytized. The vector itself, or cells pretreated with the vector, are useful in all gene medicine applications. Because it is specific for monocytic cells, the inventive vector is particularly suited to vaccine applications. Due to the ability of monocytic cells to target tumors, the inventive vector also is suitable for use in anti-tumor applications, including conventional gene therapy.

摘要翻译： 本发明的载体特别指导进入单核细胞来源的细胞。 载体由核酸组分，溶酶体逃避组分和可以吞噬的颗粒组成。 载体本身或用载体预处理的细胞可用于所有基因医学应用。 因为它是单核细胞的特异性，所以本发明的载体特别适用于疫苗应用。 由于单核细胞靶向肿瘤的能力，本发明的载体也适用于抗肿瘤应用，包括常规的基因治疗。

公开(公告)号：US20020155540A1

公开(公告)日：2002-10-24

申请号：US10074744

申请日：2002-02-13

发明人： Malla Padidam

IPC分类号： C12P021/02 ,  C12N015/87

CPC分类号： C12N15/67

摘要： This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to methods to reduce or eliminate transcriptional interference between two or more tandemly arranged genes within a host cell.

摘要翻译： 本发明涉及生物技术或遗传工程领域。 具体地，本发明涉及基因表达领域。 更具体地，本发明涉及减少或消除宿主细胞内两个或多个串联排列的基因之间的转录干扰的方法。

公开(公告)号：US20020155423A1

公开(公告)日：2002-10-24

申请号：US10048536

申请日：2002-03-13

发明人： Hideyuki Okano ,  Kazunobu Sawamoto ,  Kazuto Kobayashi ,  Natsuki Matsushita

IPC分类号： C12Q001/00 ,  C12Q001/68 ,  C12N005/08 ,  C12N015/87

CPC分类号： C12N5/0619 ,  C12N2503/02 ,  C12N2510/00

摘要： The invention of this application provides a method comprising introducing a reporter nucleic acid molecule that expresses a fluorescent protein under control of the promoter/enhancer of a gene that is expressed in dopaminergic neurons, into each of cells, and isolating fluorescence-emitting cells. The invention also provides a method for visualizing and identifying dopaminergic neurons alive that exist with in cells, which comprises introducing the above-mentioned reporter nucleic acid molecule into each of cells, and measuring the fluorescence distribution within the cells. The invention further provides a method for identifying a dopaminergic neurons-inducing factor, which comprises introducing the reporter nucleic acid molecule into cells that have the ability to differentiate into dopaminergic neurons, then incubating the cells with a candidate substance, and determining whether the candidate substance is a dopaminergic neurons-inducing factor by using the fluorescence of the cells as an indicator.

摘要翻译： 本申请的发明提供了一种方法，其包括将表达在多巴胺能神经元中表达的基因的启动子/增强子的控制下的表达荧光蛋白的报道分子核酸分子引入每个细胞中，并分离荧光发射细胞。 本发明还提供了一种用于可视化和鉴定存在于细胞中的多巴胺能神经元的方法，其包括将上述报道核酸分子引入每个细胞中，并测量细胞内的荧光分布。 本发明还提供了一种鉴定多巴胺能神经元诱导因子的方法，该方法包括将报告核酸分子引入具有分化为多巴胺能神经元的能力的细胞中，然后将细胞与候选物质一起温育，并确定候选物质 是通过使用细胞的荧光作为指标的多巴胺能神经元诱导因子。

公开(公告)号：US20020151062A1

公开(公告)日：2002-10-17

申请号：US10025423

申请日：2001-12-18

发明人： Ronald N. Zuckermann ,  Christoph J. Reinhard ,  Anne B. Jefferson ,  Eric Beausoleil

IPC分类号： A61K048/00 ,  C12N015/87

CPC分类号： A61K51/0491 ,  C07K14/001 ,  C12N15/87 ,  C12N15/88 ,  Y02P20/582

摘要： Different-sequence peptoids, including lipid- and sterol-conjugated peptoids, are found to be effective in transfection of cells with oligonucleotides. Combinatorial libraries of such peptoids can be screened efficiently in a high-throughput format, and selected peptoids are identified post-screening.

摘要翻译： 发现不同序列的拟肽，包括脂质和固醇共轭的拟肽，在用寡核苷酸转染细胞中是有效的。 可以以高通量格式有效筛选此类拟肽的组合库，筛选后鉴定选择的拟肽。