Production of horseshoe crab amebocytes in vitro
    72.
    发明授权
    Production of horseshoe crab amebocytes in vitro 失效
    生产马尾松蛋白AMEBOCYTES在体外

    公开(公告)号:US5082782A

    公开(公告)日:1992-01-21

    申请号:US290160

    申请日:1988-12-27

    CPC classification number: C12N5/0601 C12N2531/00

    Abstract: A method for producing horseshoe crab amebocytes in vitro by isolating amebocyte producing tissue from the gill flaps of horseshoe crabs, especially Limulus polyphemus. In contrast to all previous attempts to isolate and culture amebocytes from Limulus for the production of pyrogen-sensitive lysate, an effective method and means has been developed utilizing the discovery that the gill flaps are the source of the cells which differentiate into competent amebocytes. Once isolated, the tissue can be cultured long term in vitro on an artificial surface or as part of the gill flap leaflets, opened along one edge to allow access of the media to the developing amebocytes. Amebocytes are removed from the gill flaps by pulsing with Limulus serum, copper sulfate, detergent, or combinations thereof.

    Abstract translation: 一种通过从鲎鲎,特别是鲎属的鳃皮瓣中分离产生组蛋白的组织来体外生产马蹄形褐飞虱细胞的方法。 与之前尝试从鲎分离和培养白细胞以产生热原敏感性裂解物的所有尝试相反,已经开发了一种有效的方法和手段,利用发现鳃瓣是分化为感受态的细胞的细胞的来源。 一旦分离,组织可以在人造表面上或作为鳃瓣小叶的一部分在体外长期培养,沿着一个边缘开放以允许培养基进入显影的amebo细胞。 通过用鲎血清,硫酸铜,洗涤剂或其组合脉冲从鳃瓣中除去Amebocytes。

    WIDE-FIELD PHOTOACOUSTIC (PA) IMAGING SYSTEM

    公开(公告)号:US20240389861A1

    公开(公告)日:2024-11-28

    申请号:US18674020

    申请日:2024-05-24

    Abstract: A photoacoustic (PA) sensor device has a localization feature that defines a position of the sensor or scan information with a coordinate frame defining relative positions a scanned specimen and sensor information of other imaging planes of the scanned specimen. The coordinate frame is defined by positional information from localization markers at a known offset from the sensor, or from position signals from a robotic actuator driving the sensor. A processor coalesces the sensor information and positional information to reconstruct a 3-dimensional rendered structure by stitching together the sensor information to form a continuous rendering. Stitching may include adjacent imaging planes at an angular offset due to a varied pose of the sensor, and/or adjacent images over a lateral area too large for a single scan or imaging plane to capture.

    AMBIENT TEMPERATURE LIQUID METAL AIR FLOW BATTERY

    公开(公告)号:US20240372112A1

    公开(公告)日:2024-11-07

    申请号:US18654330

    申请日:2024-05-03

    Abstract: A low temperature, liquid metal approach provides a metal-air battery at room temperature or slightly above for high current density, using ambient oxygen as an electrode without the need for high heat for an opposed metal electrode. A metal-air battery employs a low melting point metal such as gallium for an all-fluid battery having a flowing aqueous electrolyte for maintaining a large volume of electrical storage capacity separate form a relatively small reactor or cell for powering an electrical load. Reversibility of the forward discharge (load powering) reaction provides a recharging capability well suited for grid storage to moderate supply and demand variations. The result is an ultra-high density, rechargeable, safe, grid-scale electricity storage technology as an alternative to lithium-ion and solvent-based flow batteries.

    OPACITY TESTING THROUGH MEASURED ILLUMINANCE
    75.
    发明公开

    公开(公告)号:US20240353430A1

    公开(公告)日:2024-10-24

    申请号:US18640521

    申请日:2024-04-19

    CPC classification number: G01N33/726 G01N21/78

    Abstract: A storage and monitoring device for red blood samples in storage for donation (blood bank) and research. Stored blood can be susceptible to degradation, most often from a phenomenon known as ice nucleation. Accurate detection and identification of relevant factors causing ice nucleation can determine healthy samples of stored blood, and identify storage factors that promote storage longevity. A photodetection approach directs an illumination source and photometer on opposed sides of a blood containing vessel for measuring an illuminance affected by hemoglobin released from ruptured cells. Hemoglobin released from ruptured cells reduces light passage though the sample to indicate unusable samples. An accelerometer and temperature sensor measure the physical and temperate factors correlating with the detected cell degradation to determine storage criteria for extending blood longevity.

    Strain gauge
    76.
    发明授权

    公开(公告)号:US12055381B2

    公开(公告)日:2024-08-06

    申请号:US17669042

    申请日:2022-02-10

    CPC classification number: G01B11/16

    Abstract: A strain gauge device employs a reflective base adapted to reflect a divergent optical signal, and a glass tube attached to the reflective base for optical communication therewith, the glass tube having a working length into which an optical fiber is inserted. The optical fiber is configured for transporting an optic signal indicative of the detected strain. The optic fiber has an outside diameter slightly smaller than an inside diameter of the glass tube for providing a slight tolerance. A distal end is disposed in slidable communication with an interior of the glass tube and accommodated by the tolerance, and a sensing circuit at the proximate end receives an optical signal indicative of strain or displacement. A resilient buffer layer made of soft silicone is disposed outside the glass tube to allow for large deformation measurements and reduce the distortion of the deformation by the introduction of the sensor.

    Method and apparatus for recycling lithium-ion batteries

    公开(公告)号:US12046725B2

    公开(公告)日:2024-07-23

    申请号:US18113130

    申请日:2023-02-23

    Abstract: Cathode material from exhausted lithium ion batteries are dissolved in a solution for extracting the useful elements Co (cobalt), Ni (nickel), Al (Aluminum) and Mn (manganese) to produce active cathode materials for new batteries. The solution includes compounds of desirable materials such as cobalt, nickel, aluminum and manganese dissolved as compounds from the exhausted cathode material of spent cells. Depending on a desired proportion, or ratio, of the desired materials, raw materials are added to the solution to achieve the desired ratio of the commingled compounds for the recycled cathode material for new cells. The desired materials precipitate out of solution without extensive heating or separation of the desired materials into individual compounds or elements. The resulting active cathode material has the predetermined ratio for use in new cells, and avoids high heat typically required to separate the useful elements because the desired materials remain commingled in solution.

    HOLLOW EXTRUSION NOZZLE
    78.
    发明公开

    公开(公告)号:US20240208141A1

    公开(公告)日:2024-06-27

    申请号:US18380500

    申请日:2023-10-16

    CPC classification number: B29C64/209 B29C64/118 B33Y10/00 B33Y30/00

    Abstract: A nozzle apparatus for a 3D printer employs a center post or shape and extrudes a circumferential, tubular form having a central void. The nozzle includes a radial arrangement of prongs or supports around a center post, which provides space for molten print medium to flow in a 360° form around the center post. The nozzle extrudes a hollow tubular form around the post that can lay a tube shaped article on a perpendicular print bed, or can be used to deposit high resolution, hollow cylindrical segments for forming lightweight articles with less volume of print medium required due to the hollow extruded shape. A stream of air or gas may be emitted into the center of the extrusion for maintaining a tubular shape. Other suitable shapes may be employed by altering the contour of the center post and the bore.

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