公开(公告)号：US11130125B2

公开(公告)日：2021-09-28

申请号：US16532825

申请日：2019-08-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Govind Kaigala ,  Robert Dean Lovchik ,  Anna Fomitcheva Khartchenko ,  Iago Pereiro Pereiro ,  Lorenzo Franco Teodoro Petrini

IPC: B01L3/00

Abstract: A method for manufacturing a fluidic device is provided. The method comprises providing a capillary, providing a structure having a fluidic channel and an opening, reducing an outer diameter of a portion of the capillary to be smaller than the opening of the structure. Furthermore, the method comprises inserting, at least partly, the portion of the capillary through the opening of the structure into the fluidic channel and applying heat to the structure to expand the inserted portion of the capillary to fit the capillary to the structure.

公开(公告)号：US20210283609A1

公开(公告)日：2021-09-16

申请号：US17333337

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Javdani Sepehri ,  Kyle Gleason

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/574

Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11104938B2

公开(公告)日：2021-08-31

申请号：US16013845

申请日：2018-06-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Dianna Maar

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6832 ,  C12Q1/6858

Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.

公开(公告)号：US20210254033A1

公开(公告)日：2021-08-19

申请号：US17225189

申请日：2021-04-08

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： XIAO-SONG GONG

IPC: C12N9/12

Abstract: Covalently-linked DNA polymerases are provided.

公开(公告)号：US11067433B2

公开(公告)日：2021-07-20

申请号：US16404637

申请日：2019-05-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Denis Pristinski

IPC: G01J1/42 ,  G02B27/10 ,  G01J1/04

Abstract: System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element including a light guide and an exit window, and at least one detector.

公开(公告)号：US20210207208A1

公开(公告)日：2021-07-08

申请号：US17148440

申请日：2021-01-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas HEREDIA ,  Dianna MAAR

IPC: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US20210207133A1

公开(公告)日：2021-07-08

申请号：US17211595

申请日：2021-03-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song GONG ,  Yan WANG

IPC: C12N15/10 ,  C12N9/22 ,  C12Q1/686

Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.

公开(公告)号：US20210207109A1

公开(公告)日：2021-07-08

申请号：US17055801

申请日：2019-05-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20210170412A1

公开(公告)日：2021-06-10

申请号：US17110095

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Donald A. MASQUELIER ,  Billy W. COLSTON, JR. ,  Benjamin J. HINDSON

IPC: B01L3/00 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F3/08 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49

Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

公开(公告)号：US11021738B2

公开(公告)日：2021-06-01

申请号：US15847408

申请日：2017-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12P19/34 ,  C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

Abstract: A method of maintaining contiguity in chromosomal DNA following treatment with a tagmentase. Conditions are selected such that the tagmentase does not release from the DNA, and thus forms a bridge linking DNA segments that have the same relationship (haplotype) as occurred in the genomic DNA. Thus the tagmentase step can occur in bulk (before partitions are formed). The resulting tagmentase-bridged DNA segments can be added to partitions maintaining the bridged segments until they are introduced into different partitions. Once in partitions, the contiguous DNA segments can be barcoded with a partition-specific barcode, thereby allowing for later identification of contiguous DNA after sequencing in bulk (after partitions contents are merged).