公开(公告)号：US20020070173A1

公开(公告)日：2002-06-13

申请号：US09732617

申请日：2000-12-08

申请人： Promega Corporation, Madison, Wisconsin

发明人： Paul E. Otto ,  Steven T. Krueger ,  Michael P. Bjerke ,  Julia E. Krueger

IPC分类号： B03C001/02

CPC分类号： B01L3/5085 ,  B01L9/523 ,  B01L2300/0829 ,  B01L2300/123 ,  B03C1/288 ,  G01N35/0098

摘要： An apparatus and method for use in magnetic separation of magnetically attractable particles suspended in a liquid. The apparatus includes a magnetic separator plate having a plurality of channels, one for each column of wells of a multi-well assay plate which is received by the magnetic separator plate. A plurality of magnets are positioned in each channel so that when the magnetic separator plate receives the multi-well assay plate, the magnets attract the magnetically attractable particles to create a pellet of material of a substance of interest on a side wall within each associated well.

摘要翻译： 用于磁悬浮在液体中的磁吸引颗粒的磁分离的装置和方法。 该装置包括具有多个通道的磁分离器板，一个用于通过磁选机板接收的多孔测定板的每列孔。 多个磁体位于每个通道中，使得当磁分离器板接收多孔测定板时，磁体吸引可磁吸引的颗粒，以在每个相关联的孔内的侧壁上产生感兴趣物质的颗粒 。

公开(公告)号：US20010031470A1

公开(公告)日：2001-10-18

申请号：US09790417

申请日：2001-02-22

申请人： Promega Corporation

发明人： John William Shultz ,  Martin K. Lewis ,  Donna Leippe ,  Michelle Mandrekar ,  Daniel Kephart ,  Richard Byron Rhodes ,  Christine Ann Andrews ,  James Robert Hartnett ,  Trent Gu ,  Ryan J. Olson ,  Keith V. Wood ,  Roy Welch

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6823 ,  C12Q1/04 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6827 ,  Y10T436/24 ,  C12Q2535/131 ,  C12Q2521/319 ,  C12Q2535/107 ,  C12Q2565/301 ,  C12Q2565/1015 ,  C12Q2537/101 ,  C12Q2565/627

摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

摘要翻译： 公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，病毒载量的测定，基因分型，医学标记诊断等。

公开(公告)号：US20230416699A1

公开(公告)日：2023-12-28

申请号：US18336636

申请日：2023-06-16

申请人： Promega Corporation

发明人： Michael Killoran ,  Ce Shi ,  Mary Hall ,  Lance P. Encell ,  Thomas Kirkland

IPC分类号： C12N9/02 ,  G01N21/76

CPC分类号： C12N9/0069 ,  C12Y113/12 ,  G01N21/763

摘要： Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

公开(公告)号：US20230382908A1

公开(公告)日：2023-11-30

申请号：US18296203

申请日：2023-04-05

申请人： PROMEGA CORPORATION

发明人： Brock Binkowski ,  Mary P. Hall ,  Thomas Machleidt ,  Joel Walker ,  Wenhui Zhou

IPC分类号： C07D471/04 ,  C07D519/00 ,  C12Q1/66 ,  G01N33/52 ,  G01N33/533 ,  G01N33/542

CPC分类号： C07D471/04 ,  C07D519/00 ,  C12Q1/66 ,  G01N33/52 ,  G01N33/533 ,  G01N33/542 ,  G01N2458/15

摘要： Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.

公开(公告)号：US11783144B2

公开(公告)日：2023-10-10

申请号：US16447289

申请日：2019-06-20

申请人： Promega Corporation

发明人： Joseph Pleshek ,  Brian George ,  Travis Phillips ,  Mark Sehmer

IPC分类号： G06K7/10 ,  G06Q10/08 ,  G08B13/24 ,  G07G1/00 ,  H04W4/021 ,  H04W4/80 ,  G06F21/35 ,  H04W4/029 ,  G06Q10/087 ,  G08B13/08 ,  H04W4/30 ,  H04W4/40

CPC分类号： G06K7/10366 ,  G06F21/35 ,  G06Q10/08 ,  G06Q10/087 ,  G07G1/009 ,  G08B13/2417 ,  H04W4/021 ,  H04W4/029 ,  H04W4/80 ,  G06F2221/2121 ,  G08B13/08 ,  H04W4/30 ,  H04W4/40

摘要： A transportable container is enabled with radio frequency identification (“RFID”) technologies to maintain automated inventory levels of those items that are inside the container. The inventory data is communicated via wireless methods, such as cellular or Wi-Fi, to a remote device such as enterprise resource planning (“ERP”) system. The ERP system may use the inventory data received from the remote device to automatically update appropriately and generate appropriate records (e.g., container inventory, restocking, invoicing, etc.). In some embodiments, automated RFID scans are triggered when the container is opened and subsequently closed. The container may include RF containment/screening to prevent inadvertent scanning of RFID tags outside of the container. The container may also include a location device to allow the location of the container to be readily determined.

公开(公告)号：US20230278968A1

公开(公告)日：2023-09-07

申请号：US18169326

申请日：2023-02-15

申请人： Promega Corporation

发明人： Ce Shi ,  Jian Cao ,  Wenhui Zhou

IPC分类号： C07D265/38 ,  C12Q1/686 ,  C12Q1/6876

CPC分类号： C07D265/38 ,  C12Q1/686 ,  C12Q1/6876

摘要： Disclosed herein are functionalized oxazine dye compounds, compositions comprising the compounds, and methods of using the compounds, e.g., in nucleic acid amplification reactions. Also disclosed herein are labeled oligonucleotides and labeled nucleotide triphosphate compounds.

公开(公告)号：US11661587B2

公开(公告)日：2023-05-30

申请号：US16537745

申请日：2019-08-12

申请人： Promega Corporation

发明人： John Michael Daly ,  Leon Michael Brownrigg ,  Jim Yu-Hsiang Tiao

IPC分类号： C12N9/02 ,  C12Q1/66 ,  C12N15/53 ,  C07K16/40

CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C12Y113/12

摘要： Disclosed are luciferase polypeptides with improved light-emitting activity and their encoding nucleic acids. These molecules are useful in a range of assays including luciferase-based gene reporter assays, bioluminescence resonance energy transfer assays, protein complementation assays and other applications in which luciferase enzymes are utilized as detectable and/or quantifiable labels. Also disclosed are methods and compositions for increasing the sensitivity and/or improving the kinetics of luciferase-catalyzed reactions as well as decreasing the impact of undesirable variables.

公开(公告)号：US11655247B2

公开(公告)日：2023-05-23

申请号：US16399410

申请日：2019-04-30

申请人： PROMEGA CORPORATION

发明人： Brock Binkowski ,  Mary P. Hall ,  Thomas Machleidt ,  Joel Walker ,  Wenhui Zhou

IPC分类号： C07D471/04 ,  C07D519/00 ,  C12Q1/66 ,  G01N33/52 ,  G01N33/533 ,  G01N33/542

CPC分类号： C07D471/04 ,  C07D519/00 ,  C12Q1/66 ,  G01N33/52 ,  G01N33/533 ,  G01N33/542 ,  G01N2458/15

摘要： Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.

公开(公告)号：US20220396830A1

公开(公告)日：2022-12-15

申请号：US17744255

申请日：2022-05-13

申请人： Promega Corporation

发明人： Wenhui Zhou ,  Hui Wang ,  Min Zhou ,  Natasha Karassina ,  James Cali ,  Jolanta Vidugiriene

IPC分类号： C12Q1/6841 ,  C12N9/14 ,  C07H19/06 ,  C07H19/14

摘要： Provided herein are reagents and methods for incorporating modified nucleotides into DNA and detecting DNA synthesis. In particular, haloalkyl-modified nucleobases are provided for incorporation into nucleic acids for detection by bioluminescent binding agents.

公开(公告)号：US11442063B2

公开(公告)日：2022-09-13

申请号：US16426982

申请日：2019-05-30

申请人： Promega Corporation

发明人： Cesear Corona ,  Poncho Meisenheimer ,  Matthew Robers ,  James Vasta

IPC分类号： G01N33/543 ,  C07D239/48 ,  G01N1/34 ,  G01N33/573 ,  G01N21/64 ,  G01R33/56 ,  G01T1/164

摘要： Provided herein are compounds that are broad-spectrum protein kinase binding agents, detectable tracers comprising such compounds, and method of use thereof for the detection of protein kinases.