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95 条记录 (耗时 0.039 秒)

    Process for delivering sirna to cardiac muscle tissue
    76.
    发明授权
    Process for delivering sirna to cardiac muscle tissue 失效
    将精神神经递送至心肌组织的过程

    公开(公告)号：US07781415B2

    公开(公告)日：2010-08-24

    申请号：US10770588

    申请日：2004-02-03

    申请人： Hans Herweijer ,  Julia Hegge ,  Jon A. Wolff ,  David L. Lewis

    发明人： Hans Herweijer ,  Julia Hegge ,  Jon A. Wolff ,  David L. Lewis

    IPC分类号： A61K48/00

    CPC分类号： A61K48/0083

    摘要： A process for delivering a polynucleotide to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a polynucleotide into a blood vessel and increasing permeability of the blood vessel to the polynucleotide. The polynucleotide can be a small interfering RNA or microRNA and inhibit gene expression in the cell.

    摘要翻译： 描述了将多核苷酸递送至哺乳动物心脏组织细胞的方法，包括将由多核苷酸组成的组合物引入血管并增加血管对多核苷酸的通透性。 多核苷酸可以是小干扰RNA或微小RNA并抑制细胞中的基因表达。

    Devices and processes for distribution of genetic material to mammalian limb
    77.
    发明授权
    Devices and processes for distribution of genetic material to mammalian limb 有权
    将遗传物质分配给哺乳动物肢体的装置和方法

    公开(公告)号：US07642248B2

    公开(公告)日：2010-01-05

    申请号：US11694140

    申请日：2007-03-30

    申请人： Vladimir G. Budker ,  Jon A. Wolff

    发明人： Vladimir G. Budker ,  Jon A. Wolff

    IPC分类号： A61K31/70

    CPC分类号： A61K48/0075

    摘要： A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from Muscular Dystrophy. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.

    摘要翻译： 描述了将治疗性多核苷酸递送至患有或可能患有肌营养不良症的肢体肌肉组织的方法。 将多核苷酸插入哺乳动物肢体血管如动脉。 通过将含有多核苷酸的溶液的注入与使用外部应用的袖带组合来提高递送效率和分布。

    Process for utilizing epitopes recognized by natural antibodies
    78.
    发明授权
    Process for utilizing epitopes recognized by natural antibodies 失效
    利用天然抗体识别的表位的方法

    公开(公告)号：US07439051B2

    公开(公告)日：2008-10-21

    申请号：US10795676

    申请日：2004-03-08

    申请人： Alexander V. Sokoloff ,  Jon A. Wolff

    发明人： Alexander V. Sokoloff ,  Jon A. Wolff

    IPC分类号： C12N7/00 ,  C12N15/00 ,  C12Q1/70

    CPC分类号： G01N33/6878 ,  G01N33/543

    摘要： The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.

    摘要翻译： 本发明涉及用于选择和使用表面暴露表位的组合物和方法。 本发明包括用于鉴定和选择具有所需性质（例如，靶向特异性，稳定性等）的肽和肽相关因子的体内和体外噬菌体肽双重显示方法。 本发明还提供了用于分离和鉴定肽特异性抗体的方法和组合物。 本发明还包括使用核定位信号用于增强核转运和DNA表达的方法和组合物。

    Stimulating vascular growth by administration of DNA sequences encoding VEGF
    79.
    发明授权
    Stimulating vascular growth by administration of DNA sequences encoding VEGF 失效
    通过施用编码VEGF的DNA序列刺激血管生长

    公开(公告)号：US06228844B1

    公开(公告)日：2001-05-08

    申请号：US08979686

    申请日：1997-11-26

    申请人： Jon A. Wolff ,  David J. Duke ,  Philip L. Felgner

    发明人： Jon A. Wolff ,  David J. Duke ,  Philip L. Felgner

    IPC分类号： A61K3800

    CPC分类号： A61K48/00 ,  C12N15/87

    摘要： The present invention provides a method for delivering a pharmaceutical polypeptide to the interior of a cardiac cell of a vertebrate in vivo, comprising the step of introducing a preparation comprising a pharmaceutically acceptable injectable carrier and naked polynucleotide operatively coding for the polypeptide into the interstitial space of the heart, whereby the naked polynucleotide is taken up into the interior of the cell and has a pharmacological effect on the vertebrate such as inducing vascular growth. In a preferred embodiment wherein the polynucleotide encodes polypeptide immunologically foreign to the vertebrate, the delivery method preferably comprises delivering an immunosuppressive agent to the vertebrate to limit immune responses directed to the polypeptide.

    摘要翻译： 本发明提供了一种用于在体内将药物多肽递送至脊椎动物心脏细胞内部的方法，其包括将包含药学上可接受的可注射载体的制剂和可操作地编码多肽的裸核苷酸引入到 心脏，由此裸露的多核苷酸被吸收到细胞的内部并且对脊椎动物具有药理作用，例如诱导血管生长。 在其中多核苷酸编码对脊椎动物免疫外源的多肽的优选实施方案中，递送方法优选包括将免疫抑制剂递送至脊椎动物以限制针对多肽的免疫应答。

    Intravascular delivery of nucleic acid
    80.
    发明授权
    Intravascular delivery of nucleic acid 失效
    核酸的血管内递送

    公开(公告)号：US07396821B1

    公开(公告)日：2008-07-08

    申请号：US09707117

    申请日：2000-11-06

    申请人： Jon A. Wolff ,  Vladimir G. Budker

    发明人： Jon A. Wolff ,  Vladimir G. Budker

    IPC分类号： A01N63/00 ,  A01N43/04 ,  A61K48/00 ,  A61K31/70

    CPC分类号： A61K31/70 ,  A61K38/00 ,  A61K48/00 ,  C12N15/87

    摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permability of the vessel is increased using an exterior cuff and immunosuppression is applied thereby the genetic material is delivered to the parenchymal cell.

    摘要翻译： 公开了将遗传物质转染入哺乳动物细胞以改变细胞的内源性质的方法。 该方法包括设计用于转染的多核苷酸。 然后将多核苷酸插入哺乳动物血管中。 在插入之前，插入之后或插入同时，使用外部袖口增加血管的渗透性，并施加免疫抑制，从而将遗传物质递送至实质细胞。