公开(公告)号：US08366896B2

公开(公告)日：2013-02-05

申请号：US12130330

申请日：2008-05-30

申请人： Takeshi Ooura ,  Ryoji Inaba

发明人： Takeshi Ooura ,  Ryoji Inaba

IPC分类号： G01N27/447

CPC分类号： G01N27/44704

摘要： Provided is a capillary electrophoresis device including a holder preventing a septum from coming off when a capillary is pulled out, and also allowing containers to be taken out in any order. In the capillary electrophoresis device for separating and analyzing a sample such as a DNA and a protein by electrophoresis, the holder includes: a septum having a capillary hole through which a capillary penetrates; a container for storing a solution; and a container-accommodation unit for accommodating the container. A hole formed in the septum engages with an engagement portion formed on the container, and thereby the septum is held to cover the container.

摘要翻译： 本发明提供一种毛细管电泳装置，其包括：当毛细管被拉出时防止隔膜脱落的保持器，并且还允许以任何顺序取出容器。 在用于通过电泳分离和分析诸如DNA和蛋白质的样品的毛细管电泳装置中，保持器包括：具有毛细管穿过的毛细孔的隔膜; 用于存储解决方案的容器; 以及用于容纳容器的容器收纳单元。 形成在隔膜上的孔与形成在容器上的接合部接合，从而保持隔膜以覆盖容器。

公开(公告)号：US08246803B2

公开(公告)日：2012-08-21

申请号：US11654683

申请日：2007-01-18

申请人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Shigeru Matsui

发明人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Shigeru Matsui

IPC分类号： G01N27/453

CPC分类号： G01N27/44721

摘要： An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus characterized in that a multi-bandpass filter is provided in an optical detection system. In one aspect of the invention, a signal detection area of a two-dimensional detector is divided into plural regions corresponding to wavelength transmission regions of the multi-bandpass filter. An integrated value of the fluorescence spectrum signal is determined in the region including a fluorescence spectrum peak of an analysis sample in the plural regions. The analysis is performed with the integrated value.

摘要翻译： 本发明的目的是提供一种毛细管电泳装置，其中可以在灵敏度和数据采集之间确保同时性，以减少上拉信号，同时在每个毛细管交换中消除光谱数据采集。 本发明涉及一种毛细管电泳装置，其特征在于在光学检测系统中提供多带通滤波器。 在本发明的一个方面，二维检测器的信号检测区域被分成与多带通滤波器的波长透射区域对应的多个区域。 在包括多个区域中的分析样品的荧光光谱峰的区域中确定荧光光谱信号的积分值。 分析用积分值进行。

公开(公告)号：US20120024707A1

公开(公告)日：2012-02-02

申请号：US13263040

申请日：2010-04-08

申请人： Takeshi Ohura ,  Ryoji Inaba ,  Manabu Akiba ,  Takashi Gomi ,  Mari Kotoura

发明人： Takeshi Ohura ,  Ryoji Inaba ,  Manabu Akiba ,  Takashi Gomi ,  Mari Kotoura

IPC分类号： G01N27/453

CPC分类号： G01N27/44743 ,  G01N27/44782

摘要： The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.

摘要翻译： 当毛细管插入或取出时，毛细管电泳装置的溶液储存装置在不延长毛细管的阴极端的情况下，将防蒸发膜牢固地固定在容器上。 溶液储存装置包括用于储存样品或溶液的容器，具有孔的盖，毛细管穿过孔覆盖容器，具有毛细孔通过的毛细孔的防蒸发膜和容器保持架 用于拿着容器。 防蒸发膜具有设置在毛细孔周边的突起，当防蒸发膜位于盖上时，防蒸发膜的突起与盖上的孔接合，并且防蒸发膜 膜由盖支撑。

公开(公告)号：US08102529B2

公开(公告)日：2012-01-24

申请号：US12637717

申请日：2009-12-14

申请人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

发明人： Richard Reel ,  Eric Nordman ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Ryoji Inaba

IPC分类号： G01J4/00

CPC分类号： G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

摘要： System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

摘要翻译： 用于荧光灯激发和检测样品的系统和方法，以提高数值孔径和/或减少荧光灯的串扰。

公开(公告)号：US07736481B2

公开(公告)日：2010-06-15

申请号：US12071835

申请日：2008-02-27

申请人： Miho Ozawa ,  Ryoji Inaba

发明人： Miho Ozawa ,  Ryoji Inaba

IPC分类号： G01N27/453 ,  G01N27/447

CPC分类号： G01N27/44704

摘要： To reduce the elongation of the migration time for the first electrophoresis cycle in successive electrophoresis cycles and improve the reliability of electrophoresis analysis. The present invention relates to making the condition of a migration medium at the start of the first electrophoresis analysis in successive electrophoresis analyses after the temperature in a thermostatic oven reaches a desired preset temperature substantially the same as the conditions after the successive electrophoresis analyses. Preferably, a voltage is applied to a separation medium filling a capillary during preheating of the thermostatic oven. Preferably, the temperature in the thermostatic oven during preheating is set higher than the temperature in the thermostatic oven during electrophoresis analysis. Preferably, a buffer solution is heated during preheating of the thermostatic oven. Preferably, the capillary is filled with a preheated separation medium during preheating of the thermostatic oven. According to the present invention, the reliability of the first electrophoresis analysis in successive electrophoresis analyses is improved.

摘要翻译： 减少连续电泳循环中第一次电泳循环的迁移时间延长，提高电泳分析的可靠性。 本发明涉及在恒温炉中的温度达到与连续电泳分析后的条件基本相同的所需预设温度后，在连续电泳分析中开始第一次电泳分析时的迁移介质条件。 优选地，在预热恒温炉期间，将电压施加到填充毛细管的分离介质上。 优选地，预热期间恒温炉中的温度设定为高于电泳分析期间恒温炉中的温度。 优选地，在预热恒温炉期间加热缓冲溶液。 优选地，在预热恒温炉期间，用预热的分离介质填充毛细管。 根据本发明，提高了连续电泳分析中的第一次电泳分析的可靠性。

公开(公告)号：US07438793B2

公开(公告)日：2008-10-21

申请号：US10812004

申请日：2004-03-30

申请人： Toshio Hanada ,  Ryoji Inaba

发明人： Toshio Hanada ,  Ryoji Inaba

IPC分类号： G01N27/453

CPC分类号： G01N27/44708 ,  G01N27/44782

摘要： A multi-capillary array which can be used for a multi-capillary electrophoresis apparatus to effectively disperse the heat generated within a load header. In a load header of the capillary array used for the multi-capillary electrophoresis, an insulated holder, a connection plate and tubular electrodes are closely arranged without substantial formation of gaps. The heat generated within the load header can be dispersed effectively to the external side by eliminating an air-layer within the load header.

摘要翻译： 一种多毛细管阵列，其可用于多毛细管电泳装置以有效地分散在负载集管内产生的热量。 在用于多毛细管电泳的毛细管阵列的负载集管中，绝缘保持器，连接板和管状电极紧密排列而没有实质上的间隙形成。 通过消除负载集管内的空气层，可以将负载集管内产生的热量有效地分散到外部。

公开(公告)号：US07422672B2

公开(公告)日：2008-09-09

申请号：US10732221

申请日：2003-12-11

申请人： Syozo Kasai ,  Takayasu Furukawa ,  Ryoji Inaba ,  Daiki Numai ,  Tomoyuki Tobita

发明人： Syozo Kasai ,  Takayasu Furukawa ,  Ryoji Inaba ,  Daiki Numai ,  Tomoyuki Tobita

IPC分类号： G01N27/453

CPC分类号： B01L7/00 ,  B01L3/5025 ,  B01L2300/0838 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0421 ,  G01N27/44726

摘要： An electrophoresis analysis method and apparatus capable of maintaining high reliability upon a repeated use of the same gel. A heat transfer medium selected from the group consisting of solids, liquids and gels is filled in substantially all of the gaps between the electrode and each capillary. A hollow electrode into which a capillary is inserted has a plurality of retaining shapes such that the capillary can be fixed at the center of the electrode. The heat from the capillary can be efficiently dissipated via the electrode, and also the temperature increase in the capillary can be prevented. Further, temperature increases due to the heating of the capillaries during operation can be controlled and thereby thermal deterioration of the gel can be prevented.

摘要翻译： 一种能够在重复使用相同凝胶时保持高可靠性的电泳分析方法和装置。 选自固体，液体和凝胶的传热介质填充在电极和每个毛细管之间的基本上所有的间隙中。 插入有毛细管的中空电极具有多个保持形状，使得毛细管可以固定在电极的中心。 可以通过电极有效地消耗来自毛细管的热量，并且可以防止毛细管中的温度升高。 此外，可以控制由于操作期间毛细管的加热引起的温度升高，从而可以防止凝胶的热劣化。

公开(公告)号：US20080202932A1

公开(公告)日：2008-08-28

申请号：US12071835

申请日：2008-02-27

申请人： Miho Ozawa ,  Ryoji Inaba

发明人： Miho Ozawa ,  Ryoji Inaba

IPC分类号： G01N27/453

CPC分类号： G01N27/44704

摘要： To reduce the elongation of the migration time for the first electrophoresis cycle in successive electrophoresis cycles and improve the reliability of electrophoresis analysis. The present invention relates to making the condition of a migration medium at the start of the first electrophoresis analysis in successive electrophoresis analyses after the temperature in a thermostatic oven reaches a desired preset temperature substantially the same as the conditions after the successive electrophoresis analyses. Preferably, a voltage is applied to a separation medium filling a capillary during preheating of the thermostatic oven. Preferably, the temperature in the thermostatic oven during preheating is set higher than the temperature in the thermostatic oven during electrophoresis analysis. Preferably, a buffer solution is heated during preheating of the thermostatic oven. Preferably, the capillary is filled with a preheated separation medium during preheating of the thermostatic oven. According to the present invention, the reliability of the first electrophoresis analysis in successive electrophoresis analyses is improved.

摘要翻译： 减少连续电泳循环中第一次电泳循环的迁移时间延长，提高电泳分析的可靠性。 本发明涉及在恒温炉中的温度达到与连续电泳分析后的条件基本相同的所需预设温度后，在连续电泳分析中开始第一次电泳分析时的迁移介质条件。 优选地，在预热恒温炉期间将电压施加到填充毛细管的分离介质上。 优选地，预热期间恒温炉中的温度设定为高于电泳分析期间恒温炉中的温度。 优选地，在预热恒温炉期间加热缓冲溶液。 优选地，在预热恒温炉期间，用预热的分离介质填充毛细管。 根据本发明，提高了连续电泳分析中的第一次电泳分析的可靠性。

公开(公告)号：US20070163882A1

公开(公告)日：2007-07-19

申请号：US11653332

申请日：2007-01-16

申请人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Isao Haraura ,  Tomoyuki Sakai

发明人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Isao Haraura ,  Tomoyuki Sakai

IPC分类号： C07K1/26 ,  G01N27/00

CPC分类号： G01N27/44704 ,  G01N27/44721

摘要： An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.

摘要翻译： 本发明的目的是提供一种毛细管电泳装置，其中在每个毛细管交换中消除光谱数据采集时，可以在灵敏度和数据采集之间确保同时性，以减小上拉信号。 本发明涉及一种毛细管电泳装置，其中通过检测毛细管位置在每个毛细管交换中检测毛细管位置偏移。 在本发明的毛细管电泳装置中设置毛细管位置测定光源。 用从毛细管位置测量光源发射的光照射毛细管，用二维检测器检测毛细管图像，从而确定毛细管的位置偏差。 基于毛细管的位置偏差，在二维检测器中设置数据获取区域，或者校正从标准位置处的毛细管确定的参考荧光光谱。