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公开(公告)号：US10538806B2
公开(公告)日：2020-01-21
申请号：US16267123
申请日：2019-02-04
Applicant: Keygene N.V.
IPC: C12P19/34 , C12Q1/6874 , G16B30/00 , C12Q1/6858 , C12Q1/6869 , C12Q1/6827 , C12Q1/6855 , C12Q1/6806 , C12Q1/6844 , C12Q1/6851
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
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公开(公告)号：US10316364B2
公开(公告)日：2019-06-11
申请号：US15707650
申请日：2017-09-18
Applicant: Keygene N.V.
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6869
Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.
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83.发明申请公开(公告)号：US20180291439A1
公开(公告)日：2018-10-11
申请号：US16000252
申请日：2018-06-05
Applicant: Keygene N.V.
IPC: C12Q1/6855 , C12Q1/6853
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.
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公开(公告)号：US20180271043A1
公开(公告)日：2018-09-27
申请号：US15757022
申请日：2016-09-02
Applicant: Keygene N.V.
Inventor： Peter Johannes VAN DIJK , Anker Preben SØRENSEN
Abstract: The present invention relates to a method for obtaining Taraxacum plants of considerable size and substantial rubber content, preferably by providing Taraxacum plants having a relatively large genome size and/or having a specific percentage of Taraxacum koksaghyz (TKS) derived genes and a specific percentage of Taraxacum officinale (TO) derived genes; selecting these plants for the presence or absence of certain markers; and subsequently selecting the plants on plant size, and on genome size.
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公开(公告)号：US09898577B2
公开(公告)日：2018-02-20
申请号：US15729328
申请日：2017-10-10
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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Patent Agency Ranking
公开(公告)号：US09898576B2
公开(公告)日：2018-02-20
申请号：US15467241
申请日：2017-03-23
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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公开(公告)号：US20180023135A1
公开(公告)日：2018-01-25
申请号：US15683063
申请日：2017-08-22
Applicant: Keygene N.V.
IPC: C12Q1/68
Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.
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公开(公告)号：US20180002751A1
公开(公告)日：2018-01-04
申请号：US15707650
申请日：2017-09-18
Applicant: Keygene N.V.
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.
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公开(公告)号：US09856486B2
公开(公告)日：2018-01-02
申请号：US14648643
申请日：2013-11-29
Applicant: Keygene N.V.
Inventor： Paul Johan Diergaarde , Marinus Willem Prins , Martin De Vos
IPC: C12N15/82
CPC classification number: C12N15/8223
Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising such trichome-specific promoter or a chimeric or vector comprising such trichome-specific promoter. The invention further provides methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.
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公开(公告)号：US20170206314A1
公开(公告)日：2017-07-20
申请号：US15467241
申请日：2017-03-23
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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