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    Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
    81.
    发明申请
    Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same 有权
    具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸

    公开(公告)号：US20140223608A1

    公开(公告)日：2014-08-07

    申请号：US14347475

    申请日：2012-09-28

    Applicant: Novozymes, Inc.

    Inventor： Yu Zhang ,  Junxin Duan ,  Lan Tang

    IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC classification number: C12N9/2437 ,  C12P19/14 ,  C12P21/02

    Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

    Abstract translation: 本发明提供了具有纤维素分解增强活性的分离的多肽和编码该多肽的多核苷酸。 本发明还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum
    86.
    发明申请
    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum 审中-公开
    在镰孢镰刀菌缺乏酶突变体中产生多肽的方法

    公开(公告)号：US20140147847A1

    公开(公告)日：2014-05-29

    申请号：US14168766

    申请日：2014-01-30

    Applicant: Novozymes, Inc.

    Inventor： Jeffrey Shasky ,  Wendy Yoder

    IPC: C12N15/80 ,  C12P21/00

    CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

    Methods of increasing secretion of polypeptides having biological activity
    87.
    发明授权
    Methods of increasing secretion of polypeptides having biological activity 有权

    公开(公告)号：US08735549B2

    公开(公告)日：2014-05-27

    申请号：US14024292

    申请日：2013-09-11

    Applicant: Novozymes, Inc.

    Inventor： Sandra Merino

    IPC: C12P21/00 ,  C12P21/04 ,  C07K1/00 ,  C12P21/02

    CPC classification number: C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

    Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

    Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same
    90.
    发明申请
    Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same 有权
    具有木聚糖酶活性的多肽和编码相同的多核苷酸

    公开(公告)号：US20140113336A1

    公开(公告)日：2014-04-24

    申请号：US14122431

    申请日：2012-08-01

    Applicant: Novozymes Inc.

    Inventor： Nikolaj Spodsberg

    IPC: C12N9/24 ,  C12P19/02 ,  C12P19/14

    CPC classification number: C12N9/2482 ,  C12N9/248 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01008

    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

    Abstract translation: 本发明涉及具有木聚糖酶活性的分离的多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

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