公开(公告)号：US10975206B2

公开(公告)日：2021-04-13

申请号：US15564373

申请日：2016-04-04

Applicant: SPIBER INC.

Inventor： Kana Ishida ,  Hironori Yamamoto ,  Hiroaki Suzumura ,  Kazuhide Sekiyama

IPC: C08J3/09 ,  C07K1/14 ,  C07K14/435 ,  C08L89/00 ,  D01F1/10 ,  D01F4/02 ,  C07K14/00

Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at, least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same.

公开(公告)号：US20210095399A1

公开(公告)日：2021-04-01

申请号：US17043834

申请日：2019-04-03

Applicant: Spiber Inc.

Inventor： Yunosuke Abe ,  Shota Togashi

IPC: D01F8/02 ,  D01F4/02 ,  D01D5/32

Abstract: Provided is a side-by-side type composite fiber having a latent crimping ability, including a first component containing a modified fibroin and a second component containing a structural protein, in which the first component and the second component are joined to each other.

公开(公告)号：US10752660B2

公开(公告)日：2020-08-25

申请号：US15356915

申请日：2016-11-21

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase

IPC: C12N15/09 ,  C12N15/70 ,  C12N1/00 ,  C12P21/02 ,  C07K14/435

Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.

公开(公告)号：US20200248231A1

公开(公告)日：2020-08-06

申请号：US16641946

申请日：2018-08-28

Applicant: Spiber Inc.

Inventor： Yusuke Ito ,  Chikako Sato ,  Nozomu Yachie

IPC: C12Q1/686

Abstract: The present invention relates to a method for producing a double-stranded DNA fragment having a desired nucleotide sequence through dual asymmetric PCR (DA-PCR). The method comprises: (1) providing a plurality of oligonucleotides (sense oligonucleotides) each corresponding to a part of a sense strand of the double-stranded DNA fragment and a plurality of oligonucleotides (antisense oligonucleotides) each corresponding to a part of an antisense strand of the double-stranded DNA fragment and mixing together the oligonucleotides with equal concentrations, DNA polymerase, and dNTP to prepare a reaction mixture solution; (2) performing PCR by using the reaction mixture solution from step (1); (3) adding a primer set capable of amplifying the double-stranded DNA fragment of full length to the reaction mixture solution from step (2); and (4) performing PCR by using the reaction mixture solution from step (3).

公开(公告)号：US20190340666A1

公开(公告)日：2019-11-07

申请号：US16513767

申请日：2019-07-17

Applicant: SPIBER INC.

Inventor： Kazuhide Sekiyama ,  Goshi Uhira

IPC: G06Q30/06 ,  G06Q30/02

Abstract: An information processing method performed by a processor, the method comprises garment selecting of receiving a selection of a garment from a user, price calculating of calculating a price of the garment on the basis of a type of the selected garment and parameters relating to a design and materials set for the garment, display controlling of causing a display part to display the calculated price, and parameter changing of changing the parameters in conjunction with an input from the user. The display controlling includes causing the display part to display the price that reflects the changed parameters in response to the change of the parameters.

公开(公告)号：US20190186050A1

公开(公告)日：2019-06-20

申请号：US16326081

申请日：2017-07-24

Applicant: RIKEN ,  SPIBER INC.

Inventor： Keiji NUMATA ,  Kousuke TSUCHIYA ,  Junichi SUGAHARA ,  Takaoki ISHII ,  Hongfang CHI

IPC: D01F4/02 ,  D01F1/10 ,  D01D5/16

CPC classification number: D01F4/00 ,  D01F4/02

Abstract: Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a β-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein.

公开(公告)号：US20190177363A1

公开(公告)日：2019-06-13

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US20190135880A1

公开(公告)日：2019-05-09

申请号：US16096818

申请日：2017-04-28

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Keisuke Morita ,  Hiroyuki Nakamura

IPC: C07K14/435 ,  C12N15/86 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  D01F4/02

Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of (A)n motif equivalent to an amino acid sequence in which, at least, one or a plurality of the (A)n motifs is deleted, as compared to naturally occurring fibroin.[In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：US20190031843A1

公开(公告)日：2019-01-31

申请号：US16073103

申请日：2017-01-27

Applicant: Riken ,  Spiber Inc.

Inventor： Keiji Numata ,  Kana Ishida ,  Hironori Yamamoto

IPC: C08J5/18 ,  C07K14/435 ,  D01F4/02 ,  B29C39/00 ,  D01D10/00 ,  B29C39/02 ,  B29C71/00

Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 90% or more to obtain the molded article.

公开(公告)号：US09968682B2

公开(公告)日：2018-05-15

申请号：US14764455

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: A61K9/08 ,  A61K38/17 ,  A61L27/22 ,  A61L27/52 ,  A61K47/42 ,  A61L26/00 ,  A61L31/14 ,  A61K9/00 ,  C07K14/435 ,  A61L27/36 ,  A61L27/60

CPC classification number: A61K47/42 ,  A61K9/0087 ,  A61K38/1767 ,  A61L26/008 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/52 ,  A61L27/60 ,  A61L31/145 ,  A61L2430/06 ,  C07K14/43518

Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.