公开(公告)号：US20070178552A1

公开(公告)日：2007-08-02

申请号：US11608673

申请日：2006-12-08

申请人： W. Arathoon ,  Paul Carter ,  Anne Merchant ,  Leonard Presta

发明人： W. Arathoon ,  Paul Carter ,  Anne Merchant ,  Leonard Presta

IPC分类号： C07K16/44 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC分类号： C07K16/2863 ,  A61K38/00 ,  C07K1/22 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/46 ,  C07K16/468 ,  C07K2317/732 ,  Y10S435/972

摘要： The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.

公开(公告)号：US20070098712A1

公开(公告)日：2007-05-03

申请号：US11536951

申请日：2006-09-29

申请人： W. Arathoon ,  Paul Carter ,  Anne Merchant ,  Leonard Presta

发明人： W. Arathoon ,  Paul Carter ,  Anne Merchant ,  Leonard Presta

IPC分类号： A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06 ,  C07K16/44

CPC分类号： C07K16/00 ,  C07K16/46 ,  C07K16/468 ,  C07K2317/732

摘要： The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.

摘要翻译： 本发明涉及制备异源多聚体多肽如双特异性抗体，双特异性免疫粘附素和抗体免疫粘附素嵌合体的方法。 本发明还涉及使用该方法制备的异源多聚体。 通常，该方法提供具有与具有抗体结合结构域的每个异聚多肽相关联的共同轻链的多特异性抗体。 另外，该方法还包括将多特异性抗体引入第一多肽的界面处的第二多肽的界面上的特异性和互补相互作用，以促进异源多聚体形成并阻碍同源多聚体形成; 和/或在第二多肽的界面处的第一多肽和相应的游离含硫醇残基的界面处的游离含硫醇残基，使得在第一和第二多肽之间形成非天然存在的二硫键。 该方法允许相对于不想要的异多元反应物和同源多聚体增强所需异源多聚体的形成。

公开(公告)号：US20070059305A1

公开(公告)日：2007-03-15

申请号：US11598618

申请日：2006-11-13

申请人： Yan Wang ,  Robert Greenberg ,  Leonard Presta ,  Jonathan Pachter ,  Judith Hailey ,  Peter Brams ,  Denise Williams ,  Mohan Srinivasan ,  Diane Feingersh

发明人： Yan Wang ,  Robert Greenberg ,  Leonard Presta ,  Jonathan Pachter ,  Judith Hailey ,  Peter Brams ,  Denise Williams ,  Mohan Srinivasan ,  Diane Feingersh

IPC分类号： A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC分类号： C07K16/2863 ,  A61K2039/505 ,  C07K16/2869 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76

摘要： The present invention includes fully human, neutralizing, monoclonal antibodies against human Insulin-like Growth Factor Receptor-I (IGFR1). The antibodies are useful for treating or preventing cancer in a subject. Also included are methods of using and producing the antibodies of the invention.

公开(公告)号：US20070048315A1

公开(公告)日：2007-03-01

申请号：US11511635

申请日：2006-08-29

申请人： Leonard Presta

发明人： Leonard Presta

IPC分类号： A61K39/395 ,  C07K16/24

CPC分类号： C07K16/244 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

摘要： Engineered antibodies to human IL-23pl9 are provided, as well as uses thereof, e.g. in treatment of inflammatory, autoimmune, and proliferative disorders.

摘要翻译： 提供了针对人IL-23pl9的工程抗体，以及其用途，例如， 治疗炎症，自身免疫和增殖性疾病。

公开(公告)号：US20070048311A1

公开(公告)日：2007-03-01

申请号：US11423588

申请日：2006-06-12

申请人： Anan Chuntharapai ,  Jin Kim ,  Leonard Presta ,  Timothy Stewart

发明人： Anan Chuntharapai ,  Jin Kim ,  Leonard Presta ,  Timothy Stewart

IPC分类号： A61K39/395

CPC分类号： C07K16/249 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00 ,  G01N33/564 ,  G01N33/6866 ,  G01N2333/56 ,  G01N2800/042 ,  G01N2800/24

摘要： The present invention relates generally to the generation and characterization of neutralizing anti-IFN-α monoclonal antibodies with broad reactivity against various IFN-α subtypes. The invention further relates to the use of such anti-IFN-α antibodies in the diagnosis and treatment of disorders associated with increased expression of IFN-α, in particular, autoimmune disorders such as insulin-dependent diabetes mellitus (IDDM) and systemic lupus erythematosus (SLE).

摘要翻译： 本发明一般涉及对各种IFN-α亚型具有广泛反应性的中和抗IFN-α单克隆抗体的产生和表征。 本发明还涉及这种抗-IFN-α抗体在诊断和治疗与IFN-α表达增加相关的疾病中的用途，特别是自身免疫性疾病如胰岛素依赖性糖尿病（IDDM）和系统性红斑狼疮 （SLE）。

公开(公告)号：US07183387B1

公开(公告)日：2007-02-27

申请号：US09713425

申请日：2000-11-15

申请人： Leonard Presta

发明人： Leonard Presta

IPC分类号： C07K16/00 ,  C07K17/00 ,  G01N33/53

CPC分类号： C07K16/4291 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2887 ,  C07K2317/55 ,  C07K2317/71 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  G01N33/6857

摘要： The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.

公开(公告)号：US20070020260A1

公开(公告)日：2007-01-25

申请号：US11536186

申请日：2006-09-28

申请人： Leonard Presta

发明人： Leonard Presta

IPC分类号： A61K39/395 ,  C12P21/08 ,  C07H21/04 ,  C12N5/06 ,  C07K16/18

CPC分类号： C07K16/2896 ,  C07K16/32 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/732

摘要： The present invention concerns compositions comprising a glycoprotein having an Fc region, wherein about 80-100% of the glycoprotein in the composition comprises a mature core carbohydrate structure which lacks fucose, attached to the Fc region of the glycoprotein. The preferred glycoprotein is an antibody or immunoadhesin.

摘要翻译： 本发明涉及包含具有Fc区的糖蛋白的组合物，其中组合物中约80-100％的糖蛋白包含与糖蛋白的Fc区连接的缺少岩藻糖的成熟核心碳水化合物结构。 优选的糖蛋白是抗体或免疫粘附蛋白。

公开(公告)号：US20060194957A1

公开(公告)日：2006-08-31

申请号：US11429786

申请日：2006-05-08

申请人： Leonard Presta

发明人： Leonard Presta

IPC分类号： C07K16/28 ,  A61K39/395

CPC分类号： C07K16/18 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/2839 ,  C07K16/2887 ,  C07K16/4291 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/71 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2799/022

摘要： The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.

公开(公告)号：US20060194954A1

公开(公告)日：2006-08-31

申请号：US11429362

申请日：2006-05-05

申请人： Esohe Idusogie ,  Leonard Presta ,  Michael Mulkerrin

发明人： Esohe Idusogie ,  Leonard Presta ,  Michael Mulkerrin

IPC分类号： A61K39/395 ,  C07K16/44

CPC分类号： C07K16/4291 ,  A61K38/00 ,  A61K47/6849 ,  A61K47/6873 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2896 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/71 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00

摘要： A variant of a polypeptide comprising a human IgG Fc region is described, which variant comprises an amino acid substitution at one or more of amino acid positions 270, 322, 326, 327, 329, 331, 333 or 334 of the human IgG Fc region. Such variants display altered effector function. For example, C1q binding and/or complement dependent cytotoxicity (CDC) activity may be altered in the variant polypeptide. The application also discloses a variant of a parent polypeptide comprising a human IgG Fc region, which variant has a better binding affinity for human C1q than the parent polypeptide

摘要翻译： 描述了包含人IgG Fc区的多肽的变体，该变体包含人IgG Fc区的氨基酸位置270,322,326,327,329,331,333或334的氨基酸取代 。 这些变体显示改变的效应子功能。 例如，可以在变体多肽中改变C1q结合和/或补体依赖性细胞毒性（CDC）活性。 本申请还公开了包含人IgG Fc区的亲本多肽的变体，该变体与亲本多肽具有对人C1q的更好的结合亲和力

公开(公告)号：US20060194291A1

公开(公告)日：2006-08-31

申请号：US11429793

申请日：2006-05-08

申请人： Leonard Presta

发明人： Leonard Presta

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N5/06 ,  C07K16/28

CPC分类号： C07K16/2896 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/22 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2799/022

摘要： The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.