公开(公告)号：US08802091B2

公开(公告)日：2014-08-12

申请号：US13463924

申请日：2012-05-04

申请人： Leslie S. Johnson ,  Ling Huang ,  Paul A. Moore ,  Deryk T. Loo ,  Francine Z. Chen

发明人： Leslie S. Johnson ,  Ling Huang ,  Paul A. Moore ,  Deryk T. Loo ,  Francine Z. Chen

IPC分类号： A61K38/00

CPC分类号： C07K16/2827 ,  A61K39/39558 ,  A61K45/06 ,  A61K47/6849 ,  A61K47/6851 ,  A61K47/6855 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3023 ,  C07K16/3046 ,  C07K16/3069 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/77 ,  C07K2317/94 ,  G01N33/57484 ,  A61K2300/00

摘要： The present invention relates to antibodies that are immunoreactive to the mammalian, and more particularly, the human B7-H3 receptor and to uses thereof, particularly in the treatment of cancer and inflammation. The invention thus particularly concerns humanized B7-H3-reactive antibodies that are capable of mediating, and more preferably enhancing the activation of the immune system against cancer cells that are associated with a variety of human cancers.

摘要翻译： 本发明涉及对哺乳动物，特别是人B7-H3受体及其用途，特别是治疗癌症和炎症的免疫反应性的抗体。 因此，本发明特别涉及能够介导，更优选增强针对与多种人类癌症相关的癌细胞的免疫系统的活化的人源化B7-H3-反应性抗体。

公开(公告)号：US08795667B2

公开(公告)日：2014-08-05

申请号：US12338142

申请日：2008-12-18

申请人： Leslie S. Johnson ,  Ling Huang

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： A61K39/285 ,  A61K39/00

CPC分类号： C07K16/081 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/76

摘要： The present invention relates to improved compositions for the prevention and treatment of smallpox, and in particular to the use of compositions containing an antibody that binds to an epitope found on the MV form of the smallpox virus and an antibody that binds to an epitope found on the EV form of the smallpox virus. The invention relates to such compositions, especially to non-blood derived antibody compositions, such as chimeric or humanized antibodies, and to methods for their use in imparting passive immunity against smallpox infection to individuals at risk of smallpox virus infection or who exhibit smallpox.

摘要翻译： 本发明涉及用于预防和治疗天花的改进的组合物，特别是涉及使用含有抗体的组合物，所述组合物含有与在天花病毒MV形式上发现的表位结合的抗体，以及结合表位上发现的表位的抗体 EV形式的天花病毒。 本发明涉及这样的组合物，特别是涉及非血液衍生的抗体组合物，例如嵌合或人源化抗体，以及它们用于在天花病毒感染风险或出现天花的个体赋予对天花感染的被动免疫的方法。

公开(公告)号：US08784808B2

公开(公告)日：2014-07-22

申请号：US13367626

申请日：2012-02-07

申请人： Leslie S. Johnson ,  Ling Huang

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： A61K39/395

CPC分类号： C07K16/283 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34

摘要： The present invention relates to antibodies or fragments thereof that bind FcγRIIB with greater affinity than said antibodies or fragments binds FcγRIIA. The invention encompasses the use of such antibodies or fragments for the treatment of diseases related to loss of balance of Fc receptor mediated signaling, such as cancer, autoimmune diseases, inflammatory diseases or IgE-mediated allergic disorders. The present invention also encompasses the use of such antibodies and fragments in combination with other cancer therapies, methods of enhancing the therapeutic effect of therapeutic antibodies, and methods of enhancing efficacy of vaccine compositions.

摘要翻译： 本发明涉及以比所述抗体或片段更高的亲和力结合FcγRIIA的FcγRIIA结合FcγRIIA的抗体或其片段。 本发明包括这样的抗体或片段用于治疗与Fc受体介导的信号传导的平衡丧失有关的疾病，例如癌症，自身免疫性疾病，炎性疾病或IgE介导的过敏性疾病的用途。 本发明还包括这样的抗体和片段与其它癌症治疗组合的用途，增强治疗性抗体治疗效果的方法，以及增强疫苗组合物功效的方法。

公开(公告)号：US20140099318A1

公开(公告)日：2014-04-10

申请号：US14118523

申请日：2012-05-16

申请人： Ling Huang ,  Leslie S. Johnson

发明人： Ling Huang ,  Leslie S. Johnson

IPC分类号： C07K16/28

CPC分类号： C07K16/2809 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/71 ,  C07K2317/732 ,  C07K2317/92

摘要： CD3-binding molecules capable of binding to human and non-human CD3, and in particular to such molecules that are cross-reactive with CD3 of a non-human mammal (e.g., a cynomolgus monkey) are presented. Uses of such antibodies and antigen-binding fragments in the treatment of cancer, autoimmune and/or inflammatory diseases and other conditions are presented.

摘要翻译： 提供能够结合人和非人CD3，特别是与非人哺乳动物（例如食蟹猴）与CD3交叉反应的分子的CD3结合分子。 提出了这些抗体和抗原结合片段在治疗癌症，自身免疫和/或炎性疾病和其他病症中的用途。

公开(公告)号：US20120141476A1

公开(公告)日：2012-06-07

申请号：US13367626

申请日：2012-02-07

申请人： Leslie S. Johnson ,  Ling Huang

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： A61K39/395 ,  A61N5/00 ,  A61P35/00

CPC分类号： C07K16/283 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34

摘要： The present invention relates to antibodies or fragments thereof that bind FcγRIIB with greater affinity than said antibodies or fragments binds FcγRIIA. The invention encompasses the use of such antibodies or fragments for the treatment of diseases related to loss of balance of Fc receptor mediated signaling, such as cancer, autoimmune diseases, inflammatory diseases or IgE-mediated allergic disorders. The present invention also encompasses the use of such antibodies and fragments in combination with other cancer therapies, methods of enhancing the therapeutic effect of therapeutic antibodies, and methods of enhancing efficacy of vaccine compositions.

摘要翻译： 本发明涉及以比所述抗体或片段更高的亲和力结合FcγRIIA的FcγRIIA结合FcγRIIA的抗体或其片段。 本发明包括这样的抗体或片段用于治疗与Fc受体介导的信号传导的平衡丧失有关的疾病，例如癌症，自身免疫性疾病，炎性疾病或IgE介导的过敏性疾病的用途。 本发明还包括这样的抗体和片段与其它癌症治疗组合的用途，增强治疗性抗体治疗效果的方法，以及增强疫苗组合物功效的方法。

公开(公告)号：US07972837B2

公开(公告)日：2011-07-05

申请号：US12042762

申请日：2008-03-05

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： G01N33/53

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US20110097323A1

公开(公告)日：2011-04-28

申请号：US12933885

申请日：2009-03-25

申请人： Leslie S. Johnson ,  Ling Huang ,  Nadine Tuaillon ,  Ezio Bonvini

发明人： Leslie S. Johnson ,  Ling Huang ,  Nadine Tuaillon ,  Ezio Bonvini

IPC分类号： A61K39/395 ,  C07K16/28 ,  A61P35/04

CPC分类号： C07K16/32 ,  A61K39/39558 ,  A61K45/06 ,  A61K2039/505 ,  A61K2039/54 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/71 ,  C07K2317/732 ,  C07K2317/92

摘要： This invention relates to antibodies that specifically bind HER2/neu, and particularly chimeric 4D5 antibodies to HER2/neu, which have reduced glycosylation as compared to known 4D5 antibodies. The invention also relates to methods of using the 4D5 antibodies and compositions comprising them in the diagnosis, prognosis and therapy of diseases such as cancer, autoimmune diseases, inflammatory disorders, and infectious disease.

摘要翻译： 本发明涉及与已知的4D5抗体相比具有降低的糖基化的HER2 / neu特异性结合HER2 / neu的嵌合4D5抗体的抗体。 本发明还涉及将4D5抗体及其组合物用于诊断，预后和治疗疾病如癌症，自身免疫性疾病，炎症性疾病和感染性疾病的方法。

公开(公告)号：US07906327B2

公开(公告)日：2011-03-15

申请号：US11503399

申请日：2006-08-10

申请人： Leslie Sydnor Johnson ,  Ling Huang

发明人： Leslie Sydnor Johnson ,  Ling Huang

IPC分类号： C12N15/00 ,  C12N1/21 ,  C12N5/07

CPC分类号： C07K16/00 ,  A01K2267/01 ,  C07K16/283 ,  C07K2317/10 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/30 ,  C12N7/00 ,  C12N15/8509 ,  C12N2795/14043

摘要： The present invention provides a dual expression vector, and methods for its use, for the expression and secretion of a full-length polypeptide of interest in eukaryotic cells, and a soluble domain or fragment of the polypeptide in bacteria. When expressed in bacteria, transcription from a bacterial promoter within a first intron and termination at the stop codon in a second intron results in expression of a fragment of the polypeptide, e.g., a Fab fragment, whereas in mammalian cells, splicing removes the bacterial regulatory sequences located in the two introns and generates the mammalian signal sequence, allowing expression of the full-length polypeptide, e.g., IgG heavy or light chain polypeptide. The dual expression vector system of the invention can be used to select and screen for new monoclonal antibodies, as well as to optimize monoclonal antibodies for binding to antigenic molecules of interest.

摘要翻译： 本发明提供双重表达载体及其用于在真核细胞中表达和分泌全长多肽的方法，以及细菌中多肽的可溶性结构域或片段。 当在细菌中表达时，来自第一内含子内的细菌启动子的转录和终止密码子在第二内含子中终止导致多肽片段的表达，例如Fab片段，而在哺乳动物细胞中，剪接除去细菌调节 位于两个内含子中的序列并产生哺乳动物信号序列，允许表达全长多肽，例如IgG重链或轻链多肽。 本发明的双表达载体系统可用于选择和筛选新的单克隆抗体，以及优化用于结合感兴趣的抗原分子的单克隆抗体。

公开(公告)号：US07786270B2

公开(公告)日：2010-08-31

申请号：US11754015

申请日：2007-05-25

申请人： Leslie S. Johnson ,  Ling Huang

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： C12P21/08

CPC分类号： A61K39/39 ,  A61K2039/505 ,  A61K2039/55516 ,  C07K16/283 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/52 ,  G01N33/564 ,  G01N33/6893 ,  G01N2800/24

摘要： The present invention relates to humanized FcγRIIB antibodies, fragments, and variants thereof that bind human FcγRIIB with a greater affinity than said antibody binds FcγRIIA. The invention encompasses the use of the humanized antibodies of the invention for the treatment of any disease related to loss of balance of Fc receptor mediated signaling, such as cancer, autoimmune and inflammatory disease. The invention provides methods of enhancing the therapeutic effect of therapeutic antibodies by administering the humanized antibodies of the invention to enhance the effector function of the therapeutic antibodies. The invention also provides methods of enhancing the efficacy of a vaccine composition by administering the humanized antibodies of the invention. The invention encompasses methods for treating an autoimmune disease and methods for elimination of cancer cells that express FcγRIIB.

摘要翻译： 本发明涉及以比所述抗体结合FcγRIIA更大的亲和力结合人FcγRIIB的人源化FcγRIIB抗体，其片段和变体。 本发明包括本发明的人源化抗体用于治疗与Fc受体介导的信号传导的平衡丧失有关的任何疾病（例如癌症，自身免疫性和炎症性疾病）的用途。 本发明提供了通过施用本发明的人源化抗体来增强治疗性抗体的治疗效果以增强治疗性抗体的效应子功能的方法。 本发明还提供了通过施用本发明的人源化抗体来增强疫苗组合物的功效的方法。 本发明包括治疗自身免疫性疾病的方法和用于消除表达FcγRIIB的癌细胞的方法。

公开(公告)号：US07776617B2

公开(公告)日：2010-08-17

申请号：US10432036

申请日：2001-11-30

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： C12Q1/00 ,  C12M1/34 ,  C12M3/00 ,  G01N33/53 ,  G01N21/00 ,  G01N21/75 ,  G01N21/76 ,  G01N21/77 ,  G01N33/566 ,  G01N33/543 ,  G01N33/551 ,  G01N33/544

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。