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117 条记录 (耗时 0.067 秒)

    Multivalent clostridial toxins
    81.
    发明授权
    Multivalent clostridial toxins 有权
    多效梭菌毒素

    公开(公告)号：US07811584B2

    公开(公告)日：2010-10-12

    申请号：US12210770

    申请日：2008-09-15

    申请人： Lance E. Steward ,  Ester Fernandez-Salas ,  Joseph Francis ,  Shengwen Li ,  Marcella A. Gilmore ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Ester Fernandez-Salas ,  Joseph Francis ,  Shengwen Li ,  Marcella A. Gilmore ,  Kei Roger Aoki

    IPC分类号： A61K39/08 ,  A61K39/38 ,  A61K39/02

    CPC分类号： A61K38/4886 ,  C07K14/33 ,  C07K2319/00 ,  C07K2319/035 ,  C07K2319/06

    摘要： The present invention is directed to multivalent Clostridial toxin comprising more than one binding domain directed to a cell surface molecule of a target cell. Such modified toxins are useful as therapeutic compositions to prevent exocytosis and secretion by the target cell. Conditions in which such compositions man be useful include, without limitation, disorders of the sensory or motor nervous system, acute or chronic pain, cancer, pancreatitis, hyperhydrosis, glandular disorders, viral infections, cystic fibrosis and the like. The invention is also directed to methods of using and administering such a composition, and methods of treating a given condition using such a composition.

    摘要翻译： 本发明涉及包含针对靶细胞的细胞表面分子的多于一个结合结构域的多价梭菌毒素。 这样的修饰毒素可用作治疗组合物，以防止靶细胞的胞吐作用和分泌。 这种组合物可用的条件包括但不限于感觉或运动神经系统的疾病，急性或慢性疼痛，癌症，胰腺炎，超氧化，腺体疾病，病毒感染，囊性纤维变性等。 本发明还涉及使用和施用这种组合物的方法，以及使用这种组合物治疗给定病症的方法。

    Cell compositions useful for cell-based FRET assays for clostridial toxins
    83.
    发明授权
    Cell compositions useful for cell-based FRET assays for clostridial toxins 有权
    用于梭菌毒素的基于细胞的FRET测定的细胞组合物

    公开(公告)号：US07749759B2

    公开(公告)日：2010-07-06

    申请号：US12192925

    申请日：2008-08-15

    申请人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    发明人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    IPC分类号： C12N5/00

    CPC分类号： C07K14/005 ,  C07K14/33 ,  C07K14/4702 ,  C07K14/4725 ,  C12N2710/16622 ,  C12N2740/16122 ,  C12Q1/37

    摘要： The present invention provides a method of determining clostridial toxin activity by (a) contacting with a sample a cell containing a clostridial toxin substrate that includes a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the donor fluorophore and the acceptor, where resonance energy transfer is exhibited between the donor fluorophore and the acceptor under the appropriate conditions; (b) exciting the donor fluorophore; and (c) determining resonance energy transfer of the contacted cell relative to a control cell, where a difference in resonance energy transfer of the contacted cell as compared to the control cell is indicative of clostridial toxin activity.

    摘要翻译： 本发明提供了一种通过（a）使样品与包含供体荧光团的梭菌毒素底物的细胞接触来测定梭菌毒素活性的方法; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 以及包含介入供体荧光团和受体之间的切割位点的梭菌毒素识别序列，其中在适当条件下在供体荧光团和受体之间显示共振能量转移; （b）激发供体荧光团; 和（c）确定接触细胞相对于对照细胞的共振能量转移，其中与对照细胞相比，所接触的细胞的共振能量转移的差异指示梭菌毒素活性。

    Fret protease assays for botulinum serotype A/E toxins
    84.
    发明授权
    Fret protease assays for botulinum serotype A/E toxins 有权
    肉毒杆菌血清型A / E毒素的Fret蛋白酶检测

    公开(公告)号：US07718766B2

    公开(公告)日：2010-05-18

    申请号：US12042458

    申请日：2008-10-31

    申请人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    IPC分类号： C07K1/00 ,  C07K2/00

    CPC分类号： G01N33/582

    摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.

    摘要翻译： 本发明提供了可用于测定任何梭菌毒素（包括所有血清型的肉毒毒素）以及破伤风毒素的蛋白酶活性的梭菌毒素底物。 本发明的梭菌毒素底物含有供体荧光团; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 和梭菌毒素识别序列，其包括切割位点，其中切割位点插入供体荧光团和受体之间，并且在适当条件下，在供体荧光团和受体之间显示共振能量转移。

    Botulinum toxin screening assays
    86.
    发明授权
    Botulinum toxin screening assays 失效
    肉毒杆菌毒素筛选试验

    公开(公告)号：US07645570B2

    公开(公告)日：2010-01-12

    申请号：US12047441

    申请日：2008-03-13

    申请人： Ester Fernandez-Salas ,  Patton E. Garay ,  Kei Roger Aoki

    发明人： Ester Fernandez-Salas ,  Patton E. Garay ,  Kei Roger Aoki

    IPC分类号： A61K39/02 ,  A61P31/00 ,  C12Q1/02

    CPC分类号： G01N33/56911 ,  G01N2333/50

    摘要： Methods for detecting BoNT/A activity in a sample, methods for screening molecules able to compete with BoNT/A receptor binding, methods for reducing BoNT/A activity in a human and methods of marketing a neurotoxin capable of selectively binding to FGFR3 to a governmental or regional regulatory authority.

    摘要翻译： 用于检测样品中BoNT / A活性的方法，用于筛选能够与BoNT / A受体结合竞争的分子的方法，用于降低人类中的BoNT / A活性的方法和将能够选择性结合FGFR3的神经毒素销售给政府的方法 或区域监管机构。

    BOTULINUM TOXIN SCREENING ASSAYS
    87.
    发明申请
    BOTULINUM TOXIN SCREENING ASSAYS 有权
    BOTULINUM TOXIN筛选测定

    公开(公告)号：US20090317839A1

    公开(公告)日：2009-12-24

    申请号：US12545647

    申请日：2009-08-21

    申请人： Ester Fernandez-Salas ,  Patton E. Garay ,  Kei Roger Aoki

    发明人： Ester Fernandez-Salas ,  Patton E. Garay ,  Kei Roger Aoki

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56911 ,  G01N2333/50

    摘要： Methods for detecting BoNT/A activity in a sample, methods for screening molecules able to compete with BoNT/A receptor binding, methods for reducing BoNT/A activity in a human and methods of marketing a neurotoxin capable of selectively binding to FGFR3 to a governmental or regional regulatory authority.

    摘要翻译： 用于检测样品中BoNT / A活性的方法，用于筛选能够与BoNT / A受体结合竞争的分子的方法，用于降低人类中的BoNT / A活性的方法和将能够选择性结合FGFR3的神经毒素销售给政府的方法 或区域监管机构。

    FRET PROTEASE ASSAYS FOR BOTULINUM SEROTYPE A/E TOXINS
    89.
    发明申请
    FRET PROTEASE ASSAYS FOR BOTULINUM SEROTYPE A/E TOXINS 失效
    用于BOTULINUM SEROTYPE A / E毒素的FRET PROTEASE测定

    公开(公告)号：US20080146786A1

    公开(公告)日：2008-06-19

    申请号：US11609189

    申请日：2006-12-11

    申请人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    IPC分类号： C07K14/195

    CPC分类号： G01N33/582

    摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.

    摘要翻译： 本发明提供了可用于测定任何梭菌毒素（包括所有血清型的肉毒毒素）以及破伤风毒素的蛋白酶活性的梭菌毒素底物。 本发明的梭菌毒素底物含有供体荧光团; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 和梭菌毒素识别序列，其包括切割位点，其中切割位点插入供体荧光团和受体之间，并且在适当条件下，在供体荧光团和受体之间显示共振能量转移。

    Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
    90.
    发明授权
    Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins 有权
    梭菌毒素的基于细胞的荧光共振能量转移（FRET）测定

    公开(公告)号：US07183066B2

    公开(公告)日：2007-02-27

    申请号：US10261161

    申请日：2002-09-27

    申请人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    发明人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    IPC分类号： C12Q1/37 ,  C07H21/04 ,  C12P21/06 ,  C12N15/74 ,  C07N14/435

    CPC分类号： C07K14/005 ,  C07K14/33 ,  C07K14/4702 ,  C07K14/4725 ,  C12N2710/16622 ,  C12N2740/16122 ,  C12Q1/37

    摘要： The present invention provides a method of determining clostridial toxin activity by (a) contacting with a sample a cell containing a clostridial toxin substrate that includes a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the donor fluorophore and the acceptor, where resonance energy transfer is exhibited between the donor fluorophore and the acceptor under the appropriate conditions; (b) exciting the donor fluorophore; and (c) determining resonance energy transfer of the contacted cell relative to a control cell, where a difference in resonance energy transfer of the contacted cell as compared to the control cell is indicative of clostridial toxin activity.

    摘要翻译： 本发明提供了一种通过（a）使样品与包含供体荧光团的梭菌毒素底物的细胞接触来测定梭菌毒素活性的方法; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 以及包含介入供体荧光团和受体之间的切割位点的梭菌毒素识别序列，其中在适当条件下在供体荧光团和受体之间显示共振能量转移; （b）激发供体荧光团; 和（c）确定接触细胞相对于对照细胞的共振能量转移，其中与对照细胞相比，所接触的细胞的共振能量转移的差异指示梭菌毒素活性。