公开(公告)号：US07828947B2

公开(公告)日：2010-11-09

申请号：US12772558

申请日：2010-05-03

申请人： Akio Oki ,  Hiroaki Oka

发明人： Akio Oki ,  Hiroaki Oka

IPC分类号： G01N27/327 ,  G01N33/92 ,  B01L3/00

CPC分类号： G01N33/6872 ,  B01L3/502707 ,  B01L3/502769

摘要： An object of the present invention is to provide a method for easily forming an artificial lipid membrane in a short period of time and an artificial lipid membrane forming apparatus suitable for carrying out such method.The present invention relates to a method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The apparatus comprises a substrate, a first spacer, a first thin film, a second spacer, a second thin film, and a cover, wherein a first chamber is formed between the substrate and the first thin film, the first thin film has a first through hole, a second chamber is formed between the first thin film and the second thin film, the second thin film has a second through hole, and the cover has an inlet. The method includes in this order a first electrolytic solution pouring step of pouring an electrolytic solution to the first chamber, a lipid solution pouring step of pouring a lipid solution to the first through hole or the second through hole, and a second electrolytic solution pouring step of pouring the electrolytic solution to the inlet to form the artificial lipid membrane inside the through hole to which the lipid solution is poured.

摘要翻译： 本发明的目的是提供一种在短时间内容易地形成人造脂质膜的方法和适于实施这种方法的人造脂膜形成装置。 本发明涉及使用人造脂膜形成装置形成人造脂膜的方法。 所述装置包括基板，第一间隔件，第一薄膜，第二间隔件，第二薄膜和盖子，其中在所述基板和所述第一薄膜之间形成第一腔室，所述第一薄膜具有第一 通孔，在第一薄膜和第二薄膜之间形成第二室，第二薄膜具有第二通孔，盖具有入口。 该方法包括以下顺序：向第一室注入电解液的第一电解溶液倾倒步骤，将脂质溶液倒入第一通孔或第二通孔的脂质溶液注入步骤，以及第二电解液倾倒步骤 将电解液注入入口，以在通过该孔注入脂质溶液之前形成人造脂质膜。

公开(公告)号：US20100216891A1

公开(公告)日：2010-08-26

申请号：US12773534

申请日：2010-05-04

申请人： Ken SHIMONO ,  Hiroaki Oka ,  Masato Suzuki ,  Kanae Senda ,  Shoji Komai

发明人： Ken SHIMONO ,  Hiroaki Oka ,  Masato Suzuki ,  Kanae Senda ,  Shoji Komai

IPC分类号： A61K31/121 ,  A61P9/12

CPC分类号： A61K31/11 ,  A61K31/045

摘要： The present invention provides an antihypertensive agent having a superior antihypertensive action, and a method for lowering blood pressure in mammals by inhalation of such an antihypertensive agent through the nostril.The antihypertensive agent of the present invention comprises citral and linalool, and has a mass ratio of citral to linalool of not less than 10:1 and not greater than 40:1. Since this antihypertensive agent has a superior antihypertensive action, it can be utilized as antihypertensive compositions for medical drugs, foods and beverages and feeds.

摘要翻译： 本发明提供了具有优异的抗高血压作用的抗高血压药，以及通过鼻孔吸入这种抗高血压药来降低哺乳动物血压的方法。 本发明的抗高血压药包括柠檬醛和芳樟醇，柠檬醛与芳樟醇的质量比不小于10:1且不大于40:1。 由于该抗高血压药具有优异的抗高血压作用，因此可用作医药，食品，饮料和饲料的抗高血压药物。

公开(公告)号：US20100133122A1

公开(公告)日：2010-06-03

申请号：US12692104

申请日：2010-01-22

申请人： Nobuhiko OZAKI ,  Hiroaki Oka

发明人： Nobuhiko OZAKI ,  Hiroaki Oka

IPC分类号： G01N27/26 ,  G01N33/50

CPC分类号： G01N33/48728

摘要： An instrument is provided for pharmacologic measurement capable of detecting a very small and short time change in electric signal caused by a pharmacologic action of a biologic specimen with quickness and a high sensitivity by steeply decreasing an external disturbance component mixed into the system while dropping/exchange of medicinal solutions is conducted in a batch mode. The pharmacologic measurement instrument detects a change in electric signal caused by a pharmacologic action or electrophysiologic action of a biologic specimen, and includes an electrically conductive box having an opening section at the top surface thereof and a well vessel disposed in the opening section thereof, wherein the well vessel includes a base having recesses in which the biologic specimens are put; measurement electrodes formed on the bottom surfaces of the respective recesses; and reference electrodes electrically insulated from the respective measurement electrodes, and the reference electrodes together with the electrically conductive box electrostatically shields the well vessel.

摘要翻译： 提供了用于药物测量的仪器，其能够通过快速和高灵敏度检测由生物样品的药理作用引起的电信号的非常小的和短的时间变化，通过急剧降低混合到系统中的外部干扰成分，同时进行下降/交换 药物溶液以批式进行。 药理测量仪器检测由生物样本的药理作用或电生理作用引起的电信号变化，并且包括其顶表面具有开口部分的导电盒和设置在其开口部分中的井管，其中 井筒包括具有其中放置生物样本的凹部的基部; 形成在各个凹部的底面上的测量电极; 以及与各测量电极电绝缘的参考电极，并且参考电极与导电盒一起静电屏蔽阱容器。

公开(公告)号：US20100116068A1

公开(公告)日：2010-05-13

申请号：US12618342

申请日：2009-11-13

申请人： Akio OKI ,  Hiroaki Oka

发明人： Akio OKI ,  Hiroaki Oka

IPC分类号： G01N1/22 ,  F23D11/32

CPC分类号： G01N1/22

摘要： The present invention of collecting gaseous sample employs the sealable container, the inlet mounted at a part of the container, the outlet mounted at the other part of the container, the atomizing electrode mounted in the container, the primary refrigerator mounted at adjacent to the atomizing electrode, the opposite electrode mounted in the container, the acicular capture electrode mounted at adjacent to the opposite electrode, and the secondary refrigerator mounted at adjacent to the capture electrode. Charged microparticles are prepared by chilling gaseous sample and producing condensate of the same. Such charged microparticles are collected by the capture electrode with static electricity, and they are condensed by chilling them. This method prevents the capture electrode from spreading solution thereon.

摘要翻译： 收集气态样品的本发明采用可密封容器，安装在容器的一部分上的入口，安装在容器的另一部分上的出口，安装在容器中的雾化电极，安装在雾化附近的主冰箱 电极，安装在容器中的相对电极，安装在相对电极附近的针状捕获电极，以及安装在捕获电极附近的次级冰箱。 通过冷却气态样品并制备其中的冷凝物来制备带电微粒。 这种带电微粒被捕获电极用静电收集，并且它们通过冷却而被冷凝。 该方法防止捕获电极在其上扩散溶液。

公开(公告)号：US20100101965A1

公开(公告)日：2010-04-29

申请号：US12683502

申请日：2010-01-07

申请人： Hidehiro SASAKI ,  Hiroaki Oka

发明人： Hidehiro SASAKI ,  Hiroaki Oka

IPC分类号： G01N27/26

CPC分类号： G01N27/3271

摘要： To provide an electrode plate for electrochemical measurements that enables detecting and quantifying the concentration of a target substance contained in a sample solution with rapidity and favorable sensitivity using an apparatus for electrochemical measurements is objected to.Specifically, the present invention is directed to a method of the determination using an electrode plate for electrochemical measurements 1 including a substrate 31, an upper layer 40 provided on the upper face of the substrate, a lower layer 11 provided on the lower face of the substrate, a first electrode body 32 sandwiched between the upper face of the substrate and the upper layer, and a second electrode body 12 sandwiched between the lower face of the substrate and the lower layer, wherein: the upper layer has a plurality of upper layer through-holes 41a and 41b; the first electrode body has a plurality of first electrodes 32d including a portion exposed from the upper face of the upper layer via the upper layer through-hole 41b; the substrate has a plurality of substrate through-holes 33; and the second electrode body has a plurality of second electrodes 12d including a portion exposed from the upper face of the upper layer via the upper layer through-hole 41a and the substrate through-hole in the second electrode body.

摘要翻译： 为了提供用于电化学测量的电极板，可以使用电化学测量装置来检测和定量检测和定量样品溶液中包含的目标物质的浓度，并以快速和良好的灵敏度。 具体而言，本发明涉及一种使用电化学测定用电极板1进行测定的方法，所述电极板包括基板31，设置在基板的上表面上的上层40，设置在基板31的下表面上的下层11 基板，夹在基板的上表面和上层之间的第一电极体32和夹在基板的下表面和下层之间的第二电极体12，其中：上层具有多个上层 通孔41a和41b; 第一电极体具有经由上层通孔41b从上层的上表面露出的部分的多个第一电极32d; 基板具有多个基板通孔33; 并且第二电极体具有多个第二电极12d，第二电极12d包括经由上层通孔41a和第二电极体中的基板通孔从上层的上表面露出的部分。

公开(公告)号：US07547415B2

公开(公告)日：2009-06-16

申请号：US11510726

申请日：2006-08-28

申请人： Yukari Hataoka ,  Tetsuo Yukimasa ,  Hiroaki Oka

发明人： Yukari Hataoka ,  Tetsuo Yukimasa ,  Hiroaki Oka

IPC分类号： B01L3/00

CPC分类号： B01L3/502738 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00891 ,  B01J2219/0093 ,  B01L3/502761 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2400/0633 ,  F16K99/0001 ,  F16K99/0003 ,  F16K99/0046 ,  F16K2099/0084 ,  Y10T137/87265 ,  Y10T436/2575

摘要： A fluid chip has a liquid support 25 for supporting a liquid 24, a fluid passage 22 formed by being surrounded by the liquid support 25 and through which the liquid 24 moves, and a plurality of magnetic particles 21 disposed in the fluid passage 24. The magnetic particles 21 are aggregated by a permanent magnet 23 so as to block the fluid passage 22 and thus cut off flow of the liquid 24. The magnetic particles 21 are dispersed along a part of a wall of the fluid passage 22 so as to open the fluid passage 22 and thus allow the liquid 24 to flow.

摘要翻译： 流体芯片具有用于支撑液体24的液体支撑件25，由液体支撑件25包围并通过液体24移动而形成的流体通道22以及设置在流体通道24中的多个磁性颗粒21。 磁性粒子21由永久磁铁23聚集，阻塞流体通道22，从而切断液体24的流动。磁性粒子21沿着流体通道22的壁的一部分分散，从而打开 流体通道22，从而允许液体24流动。

公开(公告)号：US20080257727A1

公开(公告)日：2008-10-23

申请号：US12133461

申请日：2008-06-05

申请人： Masaya NAKATANI ,  Nobuhiko Ozaki ,  Hiroaki Oka

发明人： Masaya NAKATANI ,  Nobuhiko Ozaki ,  Hiroaki Oka

IPC分类号： G01N33/48 ,  G01N27/26

CPC分类号： G01N33/48728

摘要： A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

摘要翻译： 用于测量细胞外电位的感测元件，包括衬底，设置在衬底中的阱，设置在阱的壁上的引导部分和形成在衬底的下表面处的检测电极。 指导部分是指导药物。 井在底部设置有凹陷，并且第一通孔穿过基底的凹陷和下表面。 该井用于将主体细胞，培养液和药物混合在一起。 上述构成的感测元件精确地测量由被摄体单元产生的变化。

公开(公告)号：US07396673B2

公开(公告)日：2008-07-08

申请号：US10991269

申请日：2004-11-17

申请人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

发明人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

IPC分类号： C12M1/34

CPC分类号： G01N33/48728

摘要： A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

摘要翻译： 用于测量细胞外电位的感测元件，包括衬底，设置在衬底中的阱，设置在阱的壁上的引导部分和形成在衬底的下表面处的检测电极。 指导部分是指导药物。 井在底部设置有凹陷，并且第一通孔穿过基底的凹陷和下表面。 该井用于将主体细胞，培养液和药物混合在一起。 上述构成的感测元件精确地测量由被摄体单元产生的变化。

公开(公告)号：US07351539B2

公开(公告)日：2008-04-01

申请号：US11633584

申请日：2006-12-05

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2535/125

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US20080044918A1

公开(公告)日：2008-02-21

申请号：US11607907

申请日：2006-12-04

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： G01N33/00

CPC分类号： C12Q1/6827 ,  Y10T436/143333

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.