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    HIGH THROUGHPUT PROTEIN-PROTEIN INTERACTION SCREENING IN YEAST LIQUID CULTURE
    82.
    发明申请
    HIGH THROUGHPUT PROTEIN-PROTEIN INTERACTION SCREENING IN YEAST LIQUID CULTURE 有权

    公开(公告)号：US20210002629A1

    公开(公告)日：2021-01-07

    申请号：US16856506

    申请日：2020-04-23

    Applicant: University of Washington

    Inventor： David BAKER ,  David YOUNGER ,  Eric KLAVINS

    IPC: C12N15/10

    Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

    REPEAT PROTEIN ARCHITECTURES
    84.
    发明申请
    REPEAT PROTEIN ARCHITECTURES 审中-公开

    公开(公告)号：US20190012428A1

    公开(公告)日：2019-01-10

    申请号：US16060640

    申请日：2016-12-16

    Applicant: UNIVERSITY OF WASHINGTON

    Inventor： Fabio PARMEGGIANI ,  TJ BRUNETTE ,  Po-Ssu HUANG ,  David BAKER

    IPC: G06F19/18 ,  G06F19/16 ,  G06F19/22 ,  C12N15/79

    Abstract: Methods and systems for designing proteins are disclosed, as well as proteins and protein assemblies designed. A comparing device can determine a protein repeating unit that includes one or more protein helices and one or more protein loops. The computing device can generate a protein backbone structure with a copy of the protein repeating unit. The computing device can determine whether a distance between a pair of helices of the protein backbone structure is between lower and upper distance thresholds. After determining that the distance between the pair of helices is between the lower and upper distance thresholds, the computing device can generate a plurality of protein sequences based on the protein backbone structure, select a particular protein sequence of the plurality of protein sequences based on an energy landscape that has information about energy and distance from a target fold of the particular protein sequence, and generate an output based on the particular protein sequence.

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