Schiff-base conjugate of N, N-dibutyl-P-phenylenediamine with pyridoxal 5′-phosphate for improved homocysteine assays using pyridoxal 5′-phosphate-dependent enzymes
    3.
    发明授权
    Schiff-base conjugate of N, N-dibutyl-P-phenylenediamine with pyridoxal 5′-phosphate for improved homocysteine assays using pyridoxal 5′-phosphate-dependent enzymes 有权
    N,N-二丁基对苯二胺与吡哆醛5'-磷酸盐的席夫碱缀合物用于改善使用吡哆醛5'-磷酸依赖性酶的同型半胱氨酸测定

    公开(公告)号:US09464312B2

    公开(公告)日:2016-10-11

    申请号:US14152784

    申请日:2014-01-10

    申请人: AntiCancer, Inc.

    发明人: Yuying Tan Shukuan Li

    IPC分类号: C12Q1/00 C12Q1/527 C07F9/58

    摘要: A composition, method and kit for performing a two-reagent enzymatic homocysteine assay, wherein a single homocysteinase enzyme and a Schiff-based conjugate of N,N-dibutyl-p-phenyldiamine (DBPDA) with pyridoxal 5′-phosphate (PLP) are used to measure total homocysteine in plasma or serum. The assay measures a chromophore reaction product of H2S and the DBPDA released from the Schiff-base conjugate in the presence of a Fe+3 containing compound. The resulting chromophore may be measured absorbance or fluorescence spectrophotometry.

    摘要翻译: 用于进行双试剂酶同型半胱氨酸测定的组合物,方法和试剂盒,其中N,N-二丁基对苯二胺(DBPDA)与吡哆醛5'-磷酸(PLP)的单一同型半胱氨酸酶和希夫型缀合物是 用于测量血浆或血清中的总同型半胱氨酸。 该测定法测量在含Fe + 3的化合物存在下,H 2 S和从Schiff碱偶联物释放的DBPDA的发色团反应产物。 所得到的发色团可以是测量的吸光度或荧光分光光度法。

    Skin vibration method for topical targeted delivery of beneficial agents
into hair follicles
    6.
    发明授权
    Skin vibration method for topical targeted delivery of beneficial agents into hair follicles 有权
    皮肤振动方法用于将有益剂局部靶向递送到毛囊中

    公开(公告)号:US6080127A

    公开(公告)日:2000-06-27

    申请号:US182034

    申请日:1998-10-29

    摘要: The present invention provides a methods and apparatuses for use in providing rapid, selective and effective targeted delivery of beneficial substances into hair follicles. The invention relies on the use of vibrational method of delivery of substances of different size from small molecules up to particles compatible with the diameter of hair strand into the hair bulb. The skin is subjected to the vibration of about 1 Hz to about 100 Hz frequency and 0.1 mm to about 10 mm amplitude, an amount which is sufficient to cause mechanical shifts in different directions of skin structure without damage. This results in follicle effective uptake of the tested substances such as carbon particles of different size as well as melanin. The methods and apparatuses of the present invention are particularly useful in conjunction with photosensitive agents and electromagnetic irradiation. Such a combination is used to target the delivery of the agent to within hair follicles and then subsequently activate the agent.

    摘要翻译: 本发明提供了一种方法和装置,用于提供将有益物质快速,选择性和有效地递送到毛囊中。 本发明依赖于使用不同尺寸的物质从小分子到直到发丝的直径相容的颗粒进入毛囊的振动方法。 皮肤受到频率约1Hz至约100Hz和振幅0.1mm至约10mm的振动,其量足以导致皮肤结构的不同方向的机械移动而不损坏。 这导致卵泡有效摄取测试物质,如不同大小的碳颗粒以及黑色素。 本发明的方法和装置特别适用于光敏剂和电磁照射。 这种组合用于将试剂递送到毛囊内,然后随后激活试剂。

    Native-state method and system for determining viability and
proliferative capacity of tissues in vitro
    8.
    发明授权
    Native-state method and system for determining viability and proliferative capacity of tissues in vitro 失效
    用于确定体外组织活力和增殖能力的本地状态方法和系统

    公开(公告)号:US5726009A

    公开(公告)日:1998-03-10

    申请号:US578663

    申请日:1996-06-04

    IPC分类号: G01N33/50 C12Q1/68 G01N33/53

    摘要: The present invention relates to a method of using an in vitro culture system to measure the cellular proliferative capacity cellular viability of human tissues, particularlytumor tissues. The invention also describes the use of the method to evaluate the effectiveness of an antineoplastic drug in inhibiting tumor cell proliferation of viability.

    摘要翻译: PCT No.PCT / US94 / 07476 Sec。 371日期1996年6月4日 102(e)1996年6月4日PCT PCT 1994年6月29日PCT公布。 公开号WO95 / 01455 日期1995年1月12日本发明涉及一种使用体外培养系统测量人组织,特别是肿瘤组织的细胞增殖能力细胞活力的方法。 本发明还描述了该方法用于评估抗肿瘤药物抑制肿瘤细胞生存能力增殖的有效性的用途。

    USE OF PLP WITH PEG-rMETase IN VIVO FOR ENHANCED EFFICACY
    10.
    发明申请
    USE OF PLP WITH PEG-rMETase IN VIVO FOR ENHANCED EFFICACY 审中-公开
    在血液中使用PEG-rMETase的PLP用于增强功效

    公开(公告)号:US20140205583A1

    公开(公告)日:2014-07-24

    申请号:US14162655

    申请日:2014-01-23

    申请人: AntiCancer, Inc.

    IPC分类号: A61K38/51 A61K31/675

    摘要: This invention relates to methods of modifying pyridoxal 5′ phosphate (PLP) dependent enzymes to extend the serum half-life of the enzyme, extend the in vivo period of methionine depletion in a host, and decrease the immunogenicity of the enzyme. A preferred PLP-dependent enzyme to be modified is a methioninase, preferably a recombinant methioninase (rMETase). The invention further relates to compositions comprising a modified PLP-dependent enzyme and methods of using the same.

    摘要翻译: 本发明涉及修饰吡哆醛5'磷酸(PLP)依赖性酶的方法以延长酶的血清半衰期,延长宿主中甲硫氨酸消耗的体内周期,并降低酶的免疫原性。 待修饰的优选的PLP依赖性酶是蛋氨酸酶,优选重组蛋氨酸酶(rMETase)。 本发明还涉及包含修饰的PLP依赖性酶的组合物及其使用方法。