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    USE OF PROBES FOR UNBOUND METABOLITES
    1.
    发明申请
    USE OF PROBES FOR UNBOUND METABOLITES 有权
    使用不相关代谢物的探针

    公开(公告)号：US20100062948A1

    公开(公告)日：2010-03-11

    申请号：US12447400

    申请日：2007-10-26

    申请人： Alan Marc Kleinfeld ,  Andrew Henry Huber ,  James Patrick Kampf ,  Thomas Kwan ,  Baolong Zhu

    发明人： Alan Marc Kleinfeld ,  Andrew Henry Huber ,  James Patrick Kampf ,  Thomas Kwan ,  Baolong Zhu

    IPC分类号： C40B30/04 ,  G01N33/53 ,  C12Q1/68 ,  C40B40/04

    CPC分类号： G01N33/92 ,  G01N33/728

    摘要： Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.

    摘要翻译： 公开了确定未结合代谢物水平的方法。 描述了衍生自脂肪酸结合蛋白质突变蛋白的探针，其优先结合许多未结合的代谢物，包括油酸盐，硬脂酸盐，亚油酸盐，棕榈酸盐，花生四烯酸盐和未缀合的胆红素。 使用所描述的一个或多个探针确定患者的轮廓。 该资料可用于诊断疾病，特别是心肌梗死，非酒精性脂肪性肝病（NAFLD），糖尿病，中风，败血症和新生儿黄疸。 将多个探针对测试样品的反应用于通过与由不稳定型心绞痛，非ST段抬高型心肌梗死和ST段抬高型心肌梗死产生的多探针分布进行比较来分类急性冠状动脉综合征的程度。

    Method and apparatus for ratio fluorometry
    2.
    发明授权
    Method and apparatus for ratio fluorometry 有权
    比例荧光法的方法和装置

    公开(公告)号：US06999173B2

    公开(公告)日：2006-02-14

    申请号：US10670958

    申请日：2003-09-25

    申请人： Alan M. Kleinfeld ,  Pavel V. Vodkin

    发明人： Alan M. Kleinfeld ,  Pavel V. Vodkin

    IPC分类号： G01N21/25

    CPC分类号： G01N21/645 ,  G01N2021/6421

    摘要： A method and apparatus for determining the concentration of molecules or atoms by measuring the ratio of two fluorescence signals. A sample having fluorescent molecules is exposed to radiation or excitation energy from a first source, which can be a broadband light source. The fluorescence of the sample is detected at two different wavelengths. The concentration of specific molecules or atoms within the sample is determined using the ratio of the two fluorescence signals. Fluorescent molecules can be bound to a human serum or plasma sample to allow determination of the concentration of unbound free fatty acids in the sample.

    摘要翻译： 通过测量两个荧光信号的比例来确定分子或原子的浓度的方法和装置。 具有荧光分子的样品暴露于来自可以是宽带光源的第一源的辐射或激发能。 在两个不同的波长处检测样品的荧光。 使用两个荧光信号的比例来确定样品中特定分子或原子的浓度。 荧光分子可以结合人血清或血浆样品，以测定样品中未结合游离脂肪酸的浓度。

    DEVELOPMENT AND USE OF CYSTEINE-LABELED FLUORESCENT PROBES OF UNBOUND ANALYTES
    3.
    发明申请
    DEVELOPMENT AND USE OF CYSTEINE-LABELED FLUORESCENT PROBES OF UNBOUND ANALYTES 有权
    甲基标记荧光探针的开发与应用

    公开(公告)号：US20100298162A1

    公开(公告)日：2010-11-25

    申请号：US12810505

    申请日：2009-01-16

    申请人： Alan Marc Kleinfeld ,  Andrew Henry Huber ,  James Patrick Kampf ,  Thomas Kwan ,  Baolong Zhu

    发明人： Alan Marc Kleinfeld ,  Andrew Henry Huber ,  James Patrick Kampf ,  Thomas Kwan ,  Baolong Zhu

    IPC分类号： C40B30/04 ,  C40B70/00

    CPC分类号： G01N33/92 ,  C07K14/00 ,  C07K14/47 ,  C40B30/04 ,  C40B40/10 ,  G01N33/68

    摘要： A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.

    摘要翻译： 描述了在结合未结合的分析物时在半胱氨酸残基处荧光标记并在2个波长下发生荧光比变化的高通量发现的方法。 公开了在半胱氨酸残基上标记的探针，以及用两种不同荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于流体样品中疏水物质的表征和测量，特别是未结合游离脂肪酸的表征和测量。 可以确定可以用于确定个体相对于疾病风险的个体的未结合游离脂肪酸的概况。