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公开(公告)号：US20100062948A1
公开(公告)日：2010-03-11
申请号：US12447400
申请日：2007-10-26
CPC分类号： G01N33/92 , G01N33/728
摘要： Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.
摘要翻译： 公开了确定未结合代谢物水平的方法。 描述了衍生自脂肪酸结合蛋白质突变蛋白的探针，其优先结合许多未结合的代谢物，包括油酸盐，硬脂酸盐，亚油酸盐，棕榈酸盐，花生四烯酸盐和未缀合的胆红素。 使用所描述的一个或多个探针确定患者的轮廓。 该资料可用于诊断疾病，特别是心肌梗死，非酒精性脂肪性肝病（NAFLD），糖尿病，中风，败血症和新生儿黄疸。 将多个探针对测试样品的反应用于通过与由不稳定型心绞痛，非ST段抬高型心肌梗死和ST段抬高型心肌梗死产生的多探针分布进行比较来分类急性冠状动脉综合征的程度。
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公开(公告)号：US09529003B2
公开(公告)日：2016-12-27
申请号：US14238144
申请日：2012-08-24
CPC分类号： G01N33/728 , C07K14/47 , G01N21/6428 , G01N33/533 , G01N33/582 , G01N33/92 , G01N2800/52 , Y10T436/146666
摘要： Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
摘要翻译： 描述了荧光标记并在结合胆红素时发生荧光指数变化的蛋白质的鉴定和使用。 公开了在半胱氨酸或赖氨酸残基上标记的探针，以及用两种不同的荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于测定流体样品中未结合的胆红素水平。
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公开(公告)号：US08466090B2
公开(公告)日：2013-06-18
申请号：US12535617
申请日：2009-08-04
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
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公开(公告)号：US20090318299A1
公开(公告)日：2009-12-24
申请号：US12535617
申请日：2009-08-04
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。
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公开(公告)号：US20050244864A1
公开(公告)日：2005-11-03
申请号：US11085792
申请日：2005-03-21
申请人： Alan Kleinfeld , Andrew Huber , James Kampf , Thomas Kwan , Baolong Zhu
发明人： Alan Kleinfeld , Andrew Huber , James Kampf , Thomas Kwan , Baolong Zhu
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。
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公开(公告)号：US09164109B2
公开(公告)日：2015-10-20
申请号：US12447400
申请日：2007-10-26
CPC分类号： G01N33/92 , G01N33/728
摘要： Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.
摘要翻译： 公开了确定未结合代谢物水平的方法。 描述了衍生自脂肪酸结合蛋白质突变蛋白的探针，其优先结合许多未结合的代谢物，包括油酸盐，硬脂酸盐，亚油酸盐，棕榈酸盐，花生四烯酸盐和未缀合的胆红素。 使用所描述的一个或多个探针确定患者的轮廓。 该资料可用于诊断疾病，特别是心肌梗死，非酒精性脂肪性肝病（NAFLD），糖尿病，中风，败血症和新生儿黄疸。 将多个探针对测试样品的反应用于通过与由不稳定型心绞痛，非ST段抬高型心肌梗死和ST段抬高型心肌梗死产生的多探针分布进行比较来分类急性冠状动脉综合征的程度。
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7.发明申请公开(公告)号：US20100298162A1
公开(公告)日：2010-11-25
申请号：US12810505
申请日：2009-01-16
摘要： A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.
摘要翻译： 描述了在结合未结合的分析物时在半胱氨酸残基处荧光标记并在2个波长下发生荧光比变化的高通量发现的方法。 公开了在半胱氨酸残基上标记的探针，以及用两种不同荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于流体样品中疏水物质的表征和测量，特别是未结合游离脂肪酸的表征和测量。 可以确定可以用于确定个体相对于疾病风险的个体的未结合游离脂肪酸的概况。
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公开(公告)号：US07601510B2
公开(公告)日：2009-10-13
申请号：US11085792
申请日：2005-03-21
IPC分类号： G01N33/567 , G01N33/543 , G01N33/44 , C07K14/00
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。
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9.发明授权Development and use of cysteine-labeled fluorescent probes of unbound analytes 有权开发和使用未结合分析物的半胱氨酸标记的荧光探针公开(公告)号：US09134317B2
公开(公告)日：2015-09-15
申请号：US12810505
申请日：2009-01-16
摘要： A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.
摘要翻译： 描述了在结合未结合的分析物时在半胱氨酸残基处荧光标记并在2个波长下发生荧光比变化的高通量发现的方法。 公开了在半胱氨酸残基上标记的探针，以及用两种不同荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于流体样品中疏水物质的表征和测量，特别是未结合游离脂肪酸的表征和测量。 可以确定可以用于确定个体相对于疾病风险的个体的未结合游离脂肪酸的概况。
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公开(公告)号：US20150044692A1
公开(公告)日：2015-02-12
申请号：US14238144
申请日：2012-08-24
CPC分类号： G01N33/728 , C07K14/47 , G01N21/6428 , G01N33/533 , G01N33/582 , G01N33/92 , G01N2800/52 , Y10T436/146666
摘要： Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
摘要翻译： 描述了荧光标记并在结合胆红素时发生荧光指数变化的蛋白质的鉴定和使用。 公开了在半胱氨酸或赖氨酸残基上标记的探针，以及用两种不同的荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于测定流体样品中未结合的胆红素水平。
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