公开(公告)号：US09182409B2

公开(公告)日：2015-11-10

申请号：US13425570

申请日：2012-03-21

申请人： Jonathan Stauber ,  Fabien Pamelard ,  David Bonnel ,  Grégory Hamm

发明人： Jonathan Stauber ,  Fabien Pamelard ,  David Bonnel ,  Grégory Hamm

IPC分类号： G01N24/00 ,  G01N33/68 ,  H01J49/00

CPC分类号： H01J49/164 ,  G01N33/4833 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/0027

摘要： The invention relates to a method for identifying and quantifying by mass spectrometry imaging at least one target analyte in a tissue sample, comprising the following steps: a) depositing the tissue sample to be analyzed on a support; b) analyzing the sample by mass spectrometry imaging, so as to obtain the mass spectrum of the target analyte in said tissue sample; c) weighting a signal associated with the mass spectrum of the target analyte in said tissue sample by a tissue extinction coefficient (TEC) specific to the target analyte and to the tissue sample which is obtained by using a control tissue sample; and d) using the weighted signal of the target analyte to determine the quantity of target analyte in the tissue sample.

摘要翻译： 本发明涉及通过对组织样品中的至少一种靶分析物进行质谱成像来鉴定和定量的方法，包括以下步骤：a）将待分析的组织样品沉积在载体上; b）通过质谱成像分析样品，以获得所述组织样品中目标分析物的质谱; c）通过对靶分析物特异性的组织消光系数（TEC）和通过使用对照组织样品获得的组织样品来加权与所述组织样品中的目标分析物的质谱相关联的信号; 和d）使用目标分析物的加权信号来确定组织样品中目标分析物的量。

公开(公告)号：US20140106391A1

公开(公告)日：2014-04-17

申请号：US14123128

申请日：2012-05-29

申请人： Jonathan Stauber ,  David Bonnel

发明人： Jonathan Stauber ,  David Bonnel

IPC分类号： G01N33/50

CPC分类号： G01N33/5091 ,  G01N1/38 ,  G01N33/4833 ,  G01N33/6851 ,  G01N2001/2866 ,  G01N2001/2893

摘要： The invention relates to a method for preparing a dilution range of at least one standard molecule for a tissue, whereby a tissue homogenate is used, to which the standard molecule is added, the resulting mixture being conditioned and sliced before the analysis of a slice of said tissue. The invention also relates to a method for detecting and quantifying at least one target molecule in at least one tissue using a standard range according to the invention.

摘要翻译： 本发明涉及一种制备用于组织的至少一种标准分子的稀释范围的方法，由此使用组织匀浆，向其加入标准分子，将所得混合物在分析片段之前进行调理和切片 说组织。 本发明还涉及使用根据本发明的标准范围检测和定量至少一个组织中的至少一个靶分子的方法。

公开(公告)号：US20220137027A1

公开(公告)日：2022-05-05

申请号：US17424509

申请日：2020-01-21

申请人： IMABIOTECH

发明人： JONATHAN STAUBER ,  RIMA AIT-BELKACEM ,  DAVID BONNEL ,  FABIEN PAMELARD

IPC分类号： G01N33/50 ,  G06T7/00

摘要： The present invention relates to a method for ex-vivo or in-vitro evaluation of an effect of a molecule of interest on at least one molecular marker in a dosed biological sample. The method of the invention is based on the segmentation of a biological sample based on the concentration of a molecule of interest and the comparison of molecular changes associated to the presence of the molecule of interest within different parts/segments of a same biological sample or between different biological samples, which may comprise different amounts of said molecule of interest and/or different molecules of interest.

公开(公告)号：US10132796B2

公开(公告)日：2018-11-20

申请号：US14123128

申请日：2012-05-29

申请人： Jonathan Stauber ,  David Bonnel

发明人： Jonathan Stauber ,  David Bonnel

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/68 ,  G01N1/38 ,  G01N33/483 ,  G06G7/58 ,  G01N1/28

摘要： The invention relates to a method for preparing a dilution range of at least one standard molecule for a tissue, whereby a tissue homogenate is used, to which the standard molecule is added, the resulting mixture being conditioned and sliced before the analysis of a slice of said tissue. The invention also relates to a method for detecting and quantifying at least one target molecule in at least one tissue using a standard range according to the invention.

公开(公告)号：US10141168B2

公开(公告)日：2018-11-27

申请号：US15438946

申请日：2017-02-22

申请人： IMABIOTECH

发明人： Jonathan Stauber ,  David Bonnel ,  Fabien Pamelard ,  Gael Picard De Muller

IPC分类号： H01J49/00 ,  G06T7/00

摘要： Disclosed is a method for characterizing a sample by mass spectrometry imaging (MSI) according to which a spatial arrangement of at least one ion in the sample is characterized from imaging data associated with the ion, in terms of morphology and/or texture.

公开(公告)号：US10013598B2

公开(公告)日：2018-07-03

申请号：US15110430

申请日：2015-01-09

申请人： IMABIOTECH

发明人： Fabien Pamelard ,  Jonathan M. Stauber

IPC分类号： G06K9/00 ,  G06F19/26 ,  G06F19/28 ,  G06T3/40 ,  G06T7/00 ,  H01J49/00 ,  G06T7/33 ,  G06T7/90

CPC分类号： G06K9/0014 ,  G06T3/40 ,  G06T7/0014 ,  G06T7/337 ,  G06T7/90 ,  G06T2207/10088 ,  G06T2207/10104 ,  G06T2207/30024 ,  G16B45/00 ,  G16B50/00 ,  H01J49/0036

摘要： A method for processing a plurality of spectral datasets (J1-Jn) intended for being used by a molecular imaging method or a method for recording a plurality of spectral datasets (J1-Jn), each spectral dataset (J1-Jn) being defined by a set of spatial positions (Xi, Yj) each of which is associated with a molecular spectrum with at least two dimensions containing a set of molecular information (S(Xi, Yj)), the method including in particular the following steps: for each dataset (J1-Jn), cutting the molecular spectrum associated with each position (Xi, Yj) into a plurality of spectrum segments (T1-Tm); inserting the segments (T1-Tm) obtained for each position (Xi, Yj) of each dataset (J1-Jn) into a database (BDD); selecting in the database (BDD), following a request relating to molecular information of interest, the one or more segments (T1-Tm) containing the molecular information of interest; and selecting, within each segment (T1-Tm), the molecular information of interest.

公开(公告)号：US09645138B2

公开(公告)日：2017-05-09

申请号：US14769550

申请日：2014-02-25

申请人： IMABIOTECH

发明人： Grégory Hamm ,  Jonathan Stauber

IPC分类号： G01N33/50 ,  A61K49/00

CPC分类号： G01N33/5035 ,  A61K49/0008 ,  G01N33/5082

摘要： The invention relates to a method for evaluating whether a molecule of interest binds or is incorporated in at least one target tissue, comprising visualizing and comparing the distribution of the molecule of interest and of at least one control compound within the target tissue or on the target tissue surface of at least one animal that has previously received the molecule of interest and/or the control compound. Advantageously, the distribution of the molecule of interest is visualized and compared with the distribution of positive and negative compounds using mass spectrometry imaging on the surface of the target tissue.

公开(公告)号：US20120258485A1

公开(公告)日：2012-10-11

申请号：US13425570

申请日：2012-03-21

申请人： JONATHAN STAUBER ,  Fabien Pamelard ,  David Bonnel ,  Grégory Hamm

发明人： JONATHAN STAUBER ,  Fabien Pamelard ,  David Bonnel ,  Grégory Hamm

IPC分类号： H01J49/26 ,  G01N27/62

CPC分类号： H01J49/164 ,  G01N33/4833 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/0027

摘要： The invention relates to a method for identifying and quantifying by mass spectrometry at least one target molecule in a sample, comprising the following steps: a) depositing the sample to be analyzed on a support; b) analyzing the sample by mass spectrometry, so as to obtain the mass spectrum of the target molecule in said sample; c) weighting a signal associated with the mass spectrum of the target molecule in said sample by a extinction coefficient (TEC) specific to the target molecule and to the sample; and d) using the weighted signal of the target molecule to determine the quantity of target molecule in the sample.

摘要翻译： 本发明涉及通过质谱鉴定和定量样品中至少一种靶分子的方法，其包括以下步骤：a）将待分析样品沉积在载体上; b）通过质谱分析样品，以获得所述样品中靶分子的质谱; c）通过对靶分子和样品特异性的消光系数（TEC）对与所述样品中的目标分子的质谱相关联的信号进行加权; 和d）使用靶分子的加权信号来确定样品中靶分子的量。