公开(公告)号：US07910360B2

公开(公告)日：2011-03-22

申请号：US10568701

申请日：2004-08-18

申请人： Toshiya Fujisato ,  Takuro Kogoshi ,  Yusuke Suga ,  Akio Kishida ,  Takeshi Nakatani ,  Soichiro Kitamura

发明人： Toshiya Fujisato ,  Takuro Kogoshi ,  Yusuke Suga ,  Akio Kishida ,  Takeshi Nakatani ,  Soichiro Kitamura

IPC分类号： C12M1/10 ,  C12M3/02

CPC分类号： C12M27/10

摘要： An apparatus and method for inoculating a biological substrate with cells include provision for rotating a container in which the substrate and a cell suspension are received about first and second axes of rotation. The apparatus comprises said container, first drive means for rotatably supporting the container about the first axis, and second drive means for rotatably supporting the first drive means about the second axis. The first and second axes of rotation do not lie in the same plane and extend in different directions so that the container revolves around the second axis while the container is rotating about the first axis. The inoculation method utilizes this apparatus.

摘要翻译： 用于用细胞接种生物底物的装置和方法包括提供用于旋转其中容纳基质和细胞悬浮液的容器绕第一和第二旋转轴旋转的装置。 该装置包括所述容器，用于围绕第一轴线可旋转地支撑容器的第一驱动装置和用于围绕第二轴线可旋转地支撑第一驱动装置的第二驱动装置。 第一和第二旋转轴线不位于相同的平面中并且在不同方向上延伸，使得当容器围绕第一轴线旋转时，容器围绕第二轴线旋转。 接种方法利用该装置。

公开(公告)号：US08323881B2

公开(公告)日：2012-12-04

申请号：US10562833

申请日：2004-07-02

申请人： Toshiya Fujisato ,  Akio Kishida ,  Soichiro Kitamura

发明人： Toshiya Fujisato ,  Akio Kishida ,  Soichiro Kitamura

IPC分类号： A01N1/00 ,  C12M1/00 ,  C12M3/00 ,  C12M1/36 ,  C12M1/38

CPC分类号： C12M35/00 ,  C12M21/08

摘要： A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.

摘要翻译： 将细胞注入生物组织中的方法，所述生物组织包括在微振动下的推进注射针（200）进入组织（5），所述组织通过抽吸固定到组织抽吸装置（210），并通过所述针实现细胞悬浮液注入所述组织。 还提供了用于细胞注入生物组织的装置。

公开(公告)号：US07883864B2

公开(公告)日：2011-02-08

申请号：US10538716

申请日：2003-12-11

申请人： Toshiya Fujisato ,  Akio Kishida ,  Seiichi Funamoto ,  Takeshi Nakatani ,  Soichiro Kitamura

发明人： Toshiya Fujisato ,  Akio Kishida ,  Seiichi Funamoto ,  Takeshi Nakatani ,  Soichiro Kitamura

IPC分类号： G01N1/30 ,  G01N33/48 ,  A01N1/00 ,  A01N1/02

CPC分类号： A61L27/3691 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3687 ,  A61L2430/40

摘要： A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0° C. and 40° C.

摘要翻译： 从哺乳动物来源的天然组织有效地去除或固定供体细胞的方法包括将组织浸入处理溶液中，并用微波照射组织，同时将其温度保持在0℃至40℃的范围内。

公开(公告)号：US20080300642A1

公开(公告)日：2008-12-04

申请号：US12129360

申请日：2008-05-29

申请人： Masashi INAGAKI ,  Masaru SUGIMACHI ,  Meihua LI ,  Can ZHENG ,  Masatoshi KOBAYASHI ,  Kazuo SHIMIZU

发明人： Masashi INAGAKI ,  Masaru SUGIMACHI ,  Meihua LI ,  Can ZHENG ,  Masatoshi KOBAYASHI ,  Kazuo SHIMIZU

IPC分类号： A61N1/362

CPC分类号： A61N1/36114

摘要： The cell viability in cell transplantation is improved so as to achieve sufficient repair of organ. There is provided a regeneration treatment apparatus comprising: a heart rate detector which detects a heart rate of a patient; a memory part which stores a heart rate prior to stimulation; stimulating electrodes which stimulate a vagus nerve that controls an organ having transplanted cells; and a control unit which controls an intensity of a stimulation signal to be output from the stimulating electrodes to the vagus nerve so that the heart rate of the patient detected by the heart rate detector is decreased by 5 to 20% as compared to the state prior to the stimulation.

摘要翻译： 改善细胞移植中的细胞活力，以达到器官的充分修复。 提供了一种再生处理装置，包括：心率检测器，其检测患者的心率; 存储部，其在刺激之前存储心率; 刺激电极，其刺激控制具有移植细胞的器官的迷走神经; 以及控制单元，其控制从刺激电极输出到迷走神经的刺激信号的强度，使得由心率检测器检测到的患者的心率与先前状态相比降低5至20％ 刺激。

公开(公告)号：US20080215107A1

公开(公告)日：2008-09-04

申请号：US12103786

申请日：2008-04-16

申请人： Kenji SUNAGAWA ,  Masaru SUGIMACHI ,  Takayuki SATO

发明人： Kenji SUNAGAWA ,  Masaru SUGIMACHI ,  Takayuki SATO

IPC分类号： A61N1/365 ,  A61N1/36

CPC分类号： A61N1/36114 ,  A61N1/365 ,  A61N1/36514

摘要： A medical treating system based on biological activities characterized by biological activity sensing means for sensing biological activity information produced by biological activities and outputting a biological activity signal, calculating means for receiving, analyzing, and processing the biological activity signals from the biological activity sensing means, calculating an organism stimulation signal, and outputting the organism stimulation signal, and organism stimulating means for receiving the organism stimulation signal calculated by the calculating means and stimulating an organism according to the organism stimulation signal. A cardiac pacing system based on the treating system, a blood pressure regulating system, and a cardiac disease treating system are also disclosed.

摘要翻译： 一种基于生物活性的医疗处理系统，其特征在于用于感测由生物活性产生的生物活性信息并输出生物活性信号的生物活性检测装置，用于接收，分析和处理来自生物活性检测装置的生物活性信号的计算装置， 计算生物体刺激信号，并输出生物体刺激信号，以及生物体刺激装置，用于接收由计算装置计算的生物体刺激信号，并根据生物体刺激信号刺激生物体。 还公开了一种基于治疗系统，血压调节系统和心脏疾病治疗系统的心脏起搏系统。

公开(公告)号：US20060057714A1

公开(公告)日：2006-03-16

申请号：US11252063

申请日：2005-10-18

申请人： Yasuhide Nakayama ,  Makoto Nagaishi ,  Mariko Shiba

发明人： Yasuhide Nakayama ,  Makoto Nagaishi ,  Mariko Shiba

IPC分类号： C12N15/74 ,  C12N15/00 ,  C08G63/91

CPC分类号： A61K48/0041 ,  A61K31/7088

摘要： The present invention provides a vector which can make nucleic acids to be an aggregate and locate a vector containing a cationic polymer to surround the aggregate so as to protect the nucleic acids from the enzyme. The vector comprises a polymer having branched chain(s). Preferably, 3, 4, or 6 branched chains are bonded to benzene ring. It is preferable that the number of the branched chains is higher. As the branched chain(s), a vinyl-series acrylic polymer is preferable. The vector is synthesized by reacting a dithiocarbamate compound with an acrylamide monomer for the branched chain(s).

摘要翻译： 本发明提供了一种载体，其能够使核酸成为聚集体并且定位包含阳离子聚合物的载体以包围聚集体，以便保护核酸免于酶。 载体包含具有支链的聚合物。 优选地，3,4或6个支链键合到苯环上。 支链的数目较好。 作为支链，优选乙烯基系丙烯酸类聚合物。 载体通过二硫代氨基甲酸酯化合物与支链的丙烯酰胺单体反应而合成。

公开(公告)号：US20050107868A1

公开(公告)日：2005-05-19

申请号：US10950620

申请日：2004-09-28

申请人： Yasuhide Nakayama ,  Eisuke Tatsumi ,  Yasushi Nemoto

发明人： Yasuhide Nakayama ,  Eisuke Tatsumi ,  Yasushi Nemoto

IPC分类号： A61L27/38 ,  A61L27/50 ,  A61L27/56 ,  A61F2/06 ,  A61F2/02

CPC分类号： A61L27/38 ,  A61L27/507 ,  A61L27/56

摘要： The invention provides a porous scaffold for tissue engineering which allows easy cell engraftment and cell culture and thus enables stable organization and an artificial blood vessel which exhibits high patency rate even if the inner diameter is small. The scaffold for tissue engineering is made of thermoplastic resin which forms a porous three-dimensional network structure having communication property, wherein the porous three-dimensional network structure has an average pore diameter of from 100 to 650 μm and an apparent density of from 0.01 to 0.5 g/cm3. The artificial blood vessel is composed of this scaffold. The invention provides a cuff which allows easy infiltration of cells from living subcutaneous tissues, easy engraftment of cells, and neovascularization of capillary vessels so as to obtain robust bonding with subcutaneous tissues and, as a result, ensures separation of a wounded portion from the outside, thereby blocking exacerbation factors such as bacterial infection on healing and inhibiting progression of downgrowth. That is, the invention provides a cuff with none or little infection trouble such as tunnel infection. The cuff comprises a porous three-dimensional network structure which is made of thermoplastic resin or thermosetting resin and has communication property, wherein the porous three-dimensional network structure has an average pore diameter of from 100 to 1000 μm and apparent density of from 0.01 to 0.5 g/cm3. The invention provides a biological implant covering member which allows easy infiltration of cells from living subcutaneous tissues, easy engraftment of cells, and organization, thereby obtaining robust bonding with native tissues and therefore protecting a living body from adverse effect which may occur due to the insertion of a biological implantation member into the living body. The biological implant covering member comprises a porous three-dimensional network structure which is made of thermoplastic resin or thermosetting resin and has communication property, wherein the porous three-dimensional network structure has an average pore diameter of from 100 to 1000 μm and apparent density of from 0.01 to 0.5 g/cm3.

公开(公告)号：US20100143310A1

公开(公告)日：2010-06-10

申请号：US12377056

申请日：2007-08-10

申请人： Noboru Murakami ,  Keiko Nakahara ,  Miho Hashimoto ,  Yujiro Hayashi ,  Kenji Kangawa

发明人： Noboru Murakami ,  Keiko Nakahara ,  Miho Hashimoto ,  Yujiro Hayashi ,  Kenji Kangawa

IPC分类号： A61K45/00 ,  C07K7/08 ,  C07K14/00 ,  C12N5/079 ,  A61P25/00

CPC分类号： C07K14/60 ,  A61K38/00

摘要： The invention provides a spinal neuron damage treating agent for use in the treatment of spinal neuron damage, or an agent for promoting the proliferation of spinal neuronal precursor cells in the culture of spinal neuronal precursor cells, or an agent for promoting the regeneration of spinal nerves after transplantation of cultured spinal neuronal precursor cells, and the like.The invention provides an agent that contains a substance (e.g., ghrelin) that acts on the growth hormone secretagogue-receptor as an active ingredient, the agent being a spinal neuron damage treating agent for use in the treatment of spinal neuron damage, or an agent for promoting the proliferation of cultured spinal neuronal precursor cells in the culture of spinal neuronal precursor cells, or an agent for promoting the regeneration of spinal nerves after transplantation of cultured spinal neuronal precursor cells, and the like.

摘要翻译： 本发明提供了用于治疗脊髓神经元损伤的脊髓神经元损伤治疗剂或用于促进脊髓神经元前体细胞培养中脊髓神经元前体细胞增殖的药剂，或用于促进脊髓神经再生的药剂 移植后培养的脊髓神经元前体细胞等。 本发明提供一种包含作用于生长激素促分泌素受体作为活性成分的物质（例如生长激素释放肽）的药剂，所述药剂是用于治疗脊髓神经元损伤的脊髓神经元损伤治疗剂或药剂 用于促进脊髓神经元前体细胞培养中培养的脊髓神经元前体细胞的增殖，或用于促进培养的脊髓神经元前体细胞移植后脊髓神经再生的试剂等。

公开(公告)号：US20080319502A1

公开(公告)日：2008-12-25

申请号：US12103011

申请日：2008-04-15

申请人： Kenji SUNAGAWA ,  Masaru SUGIMACHI ,  Masashi INAGAKI

发明人： Kenji SUNAGAWA ,  Masaru SUGIMACHI ,  Masashi INAGAKI

IPC分类号： A61N1/365

CPC分类号： A61N1/37288 ,  A61N1/368 ,  A61N1/37205 ,  A61N1/3756 ,  A61N1/3785 ,  H01M8/16 ,  Y02E60/527

摘要： A micro integrated cardiac pacemaker includes a control unit for outputting a control signal according to cardiograph information, heart stimulating means for stimulating heart tissue in response to the control signal, cardiograph information extracting means for extracting cardiograph information and outputting it to the control unit, and a power supply unit for supplying drive power. The power supply unit is a biological fuel cell that takes out electrons by oxidation of a biological fuel. The biological fuel cell includes an anode and a cathode. An oxidase of a biological fuel and a mediator are immobilized on the cathode. Blood and/or body fluid are used as an electrolytic solution, and a biological fuel and oxygen in the blood and/or the fluid are used. The biological fuel cell is attached to the end of a catheter and implanted into the heart, and the catheter is withdrawn, without incising the breast.

摘要翻译： 微型集成心脏起搏器包括：用于根据心电图信息输出控制信号的控制单元，响应于控制信号刺激心脏组织的心脏刺激装置;心电图仪信息提取装置，用于提取心电图信息并将其输出到控制单元;以及 用于提供驱动电力的电源单元。 电源单元是通过生物燃料的氧化而取出电子的生物燃料电池。 生物燃料电池包括阳极和阴极。 生物燃料和介体的氧化酶固定在阴极上。 使用血液和/或体液作为电解液，并且使用血液和/或流体中的生物燃料和氧气。 生物燃料电池连接到导管的末端并植入心脏，导管被取出，而不会切开乳房。

公开(公告)号：US20080139958A1

公开(公告)日：2008-06-12

申请号：US11950169

申请日：2007-12-04

申请人： Kazunori UEMURA ,  Masashi INAGAKI ,  Masaru SUGIMACHI ,  Kazuo SHIMIZU ,  Masatoshi KOBAYASHI

发明人： Kazunori UEMURA ,  Masashi INAGAKI ,  Masaru SUGIMACHI ,  Kazuo SHIMIZU ,  Masatoshi KOBAYASHI

IPC分类号： A61B5/053

CPC分类号： A61B5/029 ,  A61B5/0295 ,  A61B5/053

摘要： A practical method for estimating cardiac output and pulmonary artery wedge pressure with good accuracy is provided. The present invention provides a method for estimating the impedance arising from solid tissue by determining the impedance at the intersection between the line of identity and the extrapolated regression line, where the regression line is obtained by linearly regressing the maximum value to the minimum value of the impedance signal of each of multiple data sets, where each data set contains the maximum value and the minimum value of the impedance signal during one cardiac cycle, where impedance signal is obtained between a can electrode implanted in the left thoracic wall and an electrode inserted into the coronary vein, over a specific period of time following the infusion of hypertonic saline into the pulmonary circulation.

摘要翻译： 提供了一种准确估计心输出量和肺动脉楔形压力的实用方法。 本发明提供一种用于通过确定身份行与外推回归线之间的交叉点处的阻抗来估计由固体组织产生的阻抗的方法，其中回归线通过将最大值线性回归到最小值 多个数据组中的每一个的阻抗信号，其中每个数据集包含在一个心动周期期间的阻抗信号的最大值和最小值，其中在插入左胸壁的罐电极和插入到左胸壁的电极之间获得阻抗信号 冠状静脉，在高渗盐水输入肺循环后的特定时间段内。