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    CPMV enhancer elements
    3.
    发明授权
    CPMV enhancer elements 有权

    公开(公告)号：US11441150B2

    公开(公告)日：2022-09-13

    申请号：US15110696

    申请日：2015-01-08

    申请人： Medicago Inc.

    发明人： Pierre-Olivier Lavoie ,  Marc-Andre D'Aoust

    IPC分类号： C12N15/82 ,  C12N7/04

    摘要： An expression enhancer comprising a CPMV 5′UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160, 155, 150, or 114 of SEQ ID NO:1, or consisting of a nucleotide sequence comprising from about 80% to 100% sequence similarity with CMPVX, where X=160, 155, 150, or 114 of SEQ ID NO:1 SEQ ID NO:1 is provided. The expression enhancer may further comprise a stuffer sequence fused to the 3′ end of the 5′UTR nucleotide sequence (CMPVX+, where X=160, 155, 150, or 114 of SEQ ID NO:1). The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancer and methods using the expression enhancer are also described.

    Increasing virus-like particle yield in plants
    4.
    发明授权
    Increasing virus-like particle yield in plants 有权

    公开(公告)号：US11155581B2

    公开(公告)日：2021-10-26

    申请号：US14347804

    申请日：2012-09-28

    申请人： MEDICAGO INC.

    发明人： Marc-Andre D'aoust ,  Manon Couture ,  Louis-Philippe Vezina

    IPC分类号： C07K14/005 ,  C12N15/82 ,  A61K39/145 ,  C12N7/00 ,  C07K16/10

    摘要： A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a structural virus protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.

    Modified CPMV enhancer elements
    7.
    发明授权
    Modified CPMV enhancer elements 有权

    公开(公告)号：US10563213B2

    公开(公告)日：2020-02-18

    申请号：US15129269

    申请日：2015-03-27

    申请人： Medicago Inc.

    发明人： Marc-Andre D'Aoust ,  Pierre-Olivier Lavoie

    IPC分类号： C12N15/82 ,  C07K14/11

    摘要： An expression enhancer comprising, in series, a CPMV 5′UTR nucleotide sequence comprising nucleotides 1-160 of SEQ ID NO:1, or comprising a nucleotide sequence comprising from about with 80% to 100% sequence similarity with SEQ ID NO:1, and a stuffer fragment is provided. The stuffer fragment comprises a nucleotide sequence encoding an incomplete M protein and one or more kozak sequence active in a plant. Plants and plant matter comprising the expression enhancer and methods using the expression enhancer are also described.

    Method for recovering plant-derived proteins
    9.
    发明授权
    Method for recovering plant-derived proteins 有权

    公开(公告)号：US09815873B2

    公开(公告)日：2017-11-14

    申请号：US14006552

    申请日：2012-03-23

    申请人： Manon Couture ,  Dany Paquet ,  Louis-Philippe Vezina

    发明人： Manon Couture ,  Dany Paquet ,  Louis-Philippe Vezina

    IPC分类号： C07K14/415 ,  C07K1/14

    CPC分类号： C07K14/415 ,  C07K1/145

    摘要： Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.