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    RI T-DNA promoters
    2.
    发明授权
    RI T-DNA promoters 失效
    RI T-DNA启动子

    公开(公告)号：US5366887A

    公开(公告)日：1994-11-22

    申请号：US8216

    申请日：1993-01-25

    申请人： Jerry L. Slightom ,  David A. Tepfer

    发明人： Jerry L. Slightom ,  David A. Tepfer

    IPC分类号： C07K14/325 ,  C07K14/415 ,  C07K14/42 ,  C07K14/43 ,  C12N15/82 ,  C12N15/84 ,  C12N5/00 ,  C07H17/00 ,  C12N15/00

    CPC分类号： C07K14/415 ,  C07K14/325 ,  C07K14/42 ,  C07K14/43 ,  C12N15/8216

    摘要： The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

    摘要翻译： 公开了发根农杆菌菌株HRI和A4中发现的Ri质粒的TL-DNA序列。 发现由真核启动子，核糖体结合位点和多聚腺苷酸化位点限制的16个开放阅读框，其中5个被观察到以发育和表型调节的方式转录。 使用来自pRi TL-DNA的启动子和多聚腺苷酸化位点来控制异源外来结构基因的表达，使用菜豆（Phaseolus vulgaris）存储蛋白（菜豆蛋白），普通寻常型凝集素，甜蛋白（马齿苋）， 和苏云金芽孢杆菌晶体蛋白。 还提供了可用于操纵结构基因和T-DNA序列的载体。

    Methods of inserting viral DNA into plant material
    5.
    发明授权
    Methods of inserting viral DNA into plant material 失效
    将病毒DNA插入植物材料的方法

    公开(公告)号：US5569597A

    公开(公告)日：1996-10-29

    申请号：US272958

    申请日：1994-07-11

    申请人： Nigel H. Grimsley ,  Barbara Hohn ,  Thomas Hohn ,  Jeffrey W. Davies ,  Margaret I. Boulton

    发明人： Nigel H. Grimsley ,  Barbara Hohn ,  Thomas Hohn ,  Jeffrey W. Davies ,  Margaret I. Boulton

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/00 ,  C12N15/05

    CPC分类号： C12N15/8205 ,  A01H1/00

    摘要： The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.

    摘要翻译： 本发明涉及一种将病毒DNA（其可任选地可以含有货物-DNA）插入植物或其存活部分，但优选插入单子叶植物种类的植物，最优选地插入禾本科植物中的新方法，使用合适的转移 微生物。 本发明还包括适合于根据本发明方法的特定条件的重组DNA，质粒和载体分子以及根据所述方法可获得的转基因植物产物。

    Modified 7S legume seed storage proteins
    6.
    发明授权
    Modified 7S legume seed storage proteins 失效
    改良的7S豆科种子储存蛋白

    公开(公告)号：US5576203A

    公开(公告)日：1996-11-19

    申请号：US476743

    申请日：1995-06-07

    申请人： Leslie M. Hoffman

    发明人： Leslie M. Hoffman

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N5/14

    CPC分类号： C07K14/415 ,  C12N15/8251 ,  C12N15/8253

    摘要： The present invention discloses plant cells which contain modified 7S legume seed storage protein. Modification of 7S seed storage proteins which are expressible in plant cells and transformation of such genes into plant cells is also taught. Furthermore, methods and DNA molecules useful for producing plant cells containing modified 7S seed storage proteins are also disclosed. The invention is exemplified by insertion of an oligonucleotide encoding 15 amino acid residues, including 6 methionines, into a Phaseolus vulgaris phaseolin gene, thereby tripling its content of sulfur-containing amino acids.

    摘要翻译： 本发明公开了含有改良的7S豆科种子贮藏蛋白的植物细胞。 还教导了可在植物细胞中表达并将这些基因转化到植物细胞中的7S种子储存蛋白的修饰。 此外，还公开了可用于产生含有修饰的7S种子储存蛋白的植物细胞的方法和DNA分子。 本发明通过将编码15个氨基酸残基的寡核苷酸（包括6个甲硫氨酸）插入菜豆提取物中，从而将其含硫氨基酸的含量加倍。