摘要:
This invention provides an ELISA detection kit and method for IgA antibody against Porcine rotavirus group A, belonging to the field of animal husbandry detection technology. The ELISA detection method of this invention utilizes popular PoRV variant strains as parental strains to successfully express recombinant VP6 fusion protein in vitro. Subsequently, the VP6 fusion protein was used as an antigen to be coated onto enzyme-labeled plate to establish a novel ELISA detection method. The method provided by this invention possesses strong sensitivity, high specificity, robust stability, good reproducibility, and ease of preparation, and holds important clinical practical significance for the diagnosis, prevention and control of PoRV.
摘要:
A label-free optical biosensing system and method provide high sensitivity and specificity for in situ detection and activity estimation of serotypes, such as Botulinum Neurotoxins (BoNT). Pre-fabricated thin-film support structures are treated with a competitive immunoassay coupled to a biochemical cascade reaction, which provides optical signal amplification. When the thin-film support structures receive a target analyte and are exposed to polychromatic light, reaction products cause a change in average refractive index which appears in reflectivity spectra measured by an optical interferometer. Optical signal amplification enables a linear response for serotype concentrations of only a few picograms per millilitre, as well as a level-of-detection threshold of 5.0 picograms per millilitre or less. The specificity and selectivity of the method have been verified in studies using various combinations of different serotypes as a target analyte. Similarly, the serotype activity is estimated by an adjunct sensing platform.
摘要:
A method of determining the feed efficiency and methane production of a ruminating animal comprising analyzing the number and/or diversity of a bacterial taxon of a microbiome of the animal or of a gene content of said microbiome, wherein a number and/or diversity of said taxon below a predetermined level is indicative of an animal having a high feed efficiency and low methane production, or a number of genes below a predetermined level is indicative of an animal having a high feed efficiency and low methane production.
摘要:
A feature extraction method of fruit spectrum includes taking a vector of each wavelength point in spectrum of samples as source data, and acquiring a sorting of all vectors by processing the source data by SPA; according to the sorting of the vectors, acquiring distribution points of each sample on a coordinate system; acquiring classification results of the samples by destructive analysis, and acquiring a number of first sample categories; acquiring a first Euclidean distance between the first sample categories; according to a sorting of the wavelength points, acquiring distribution points of each sample on the coordinate system; acquiring a number of second sample categories; acquiring a second Euclidean distance between the second sample categories; determining whether the first Euclidean distance is less than the second Euclidean distance; determine a (M+2)-th vector to be valid or invalid based on a comparison result.
摘要:
Apparatus and method for detecting a dissolved gaseous impurity in an aqueous environment, comprises a tube for isolating liquid surface, or a sampler for obtaining a liquid sample from the aqueous environment, a vacuum pump located to exert a vacuum, leaving the surface to evaporate into the vacuum; and a holding compartment for holding evaporated gas which may then be analyzed using electrochemical or spectroscopic methods. The apparatus is useful for detecting levels of ammonia in fish ponds or indeed any impurity that may be dissolved in the water.
摘要:
‘ANABP 01’ is a new and distinct apple variety (Malus domestica Borkh.) notable for its attractive dark purple red skin color, excellent flavor and texture, attractive size and shape, and consistent cropping characteristics.
摘要:
Plant expression construct are provided. According to an embodiment, the plant expression construct comprises a nucleic acid sequence encoding a hexokinase under a transcriptional control of a guard cell-specific cis-acting regulatory element. Also provided are methods of using the constructs and transgenic plants, plant cells and plant parts expressing same.
摘要:
A method of killing a phytopathogen or reducing growth thereof is provided. The method comprising exposing the phytopathogen to an effective amount of a composition comprising a biologically pure culture of Daldinia sp. or at least one volatile organic compound (VOC) being produced by the biologically pure culture and capable of killing the phytopathogen or reducing growth thereof.
摘要:
A feature extraction method of fruit spectrum includes taking a vector of each wavelength point in spectrum of samples as source data, and acquiring a sorting of all vectors by processing the source data by SPA; according to the sorting of the vectors, acquiring distribution points of each sample on a coordinate system; acquiring classification results of the samples by destructive analysis, and acquiring a number of first sample categories; acquiring a first Euclidean distance between the first sample categories; according to a sorting of the wavelength points, acquiring distribution points of each sample on the coordinate system; acquiring a number of second sample categories; acquiring a second Euclidean distance between the second sample categories; determining whether the first Euclidean distance is less than the second Euclidean distance; determine a (M+2)-th vector to be valid or invalid based on a comparison result.
摘要:
A grafting method includes: making a cut surface of a scion and a cut surface of a rootstock contact with each other, and disposing sheet-like thermoplastic resin films facing each other across a contact part; and covering a periphery of the contact part with the thermoplastic resin films by ultrasonic ally welding the thermoplastic resin films.