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公开(公告)号：US20150166992A1
公开(公告)日：2015-06-18
申请号：US14564573
申请日：2014-12-09
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A61K31/713 , A61K47/50 , C12N15/111 , C12N2310/113 , C12N2310/141 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2310/332 , C12N2310/3515 , C12N2310/3519 , C12N2310/51 , C12N2310/531 , C12N2330/31
摘要： The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.
摘要翻译： 本发明一般涉及通过序列特异性结合抑制靶核酸功能的组合物和方法。 组合物和方法可用于抑制微RNA和其他相对较短的非编码RNA。
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公开(公告)号：US20110251261A1
公开(公告)日：2011-10-13
申请号：US13085878
申请日：2011-04-13
CPC分类号： C12N15/113 , A61K31/713 , A61K47/50 , C12N15/111 , C12N2310/113 , C12N2310/141 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2310/332 , C12N2310/3515 , C12N2310/3519 , C12N2310/51 , C12N2310/531 , C12N2330/31
摘要： The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.
摘要翻译： 本发明一般涉及通过序列特异性结合抑制靶核酸功能的组合物和方法。 组合物和方法可用于抑制微RNA和其他相对较短的非编码RNA。
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3.发明授权siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof 有权用于减少RNAi中的靶外表型效应的siRNA序列非依赖性修饰形式及其稳定形式公开(公告)号：US08524681B2
公开(公告)日：2013-09-03
申请号：US12727336
申请日：2010-03-19
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1137 , C12N15/1138 , C12N2310/14 , C12N2310/319 , C12N2310/321 , C12N2310/3231 , C12N2310/343 , C12N2320/53 , C12N2310/3521
摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
摘要翻译： 提供了具有修饰的核苷酸的修饰形式用于siRNA。 具有修饰形式的短干扰RNA和本文提供的经修饰的核苷酸减少RNA干扰内源基因的脱靶效应。 证明进一步修饰格式化的siRNA被稳定到富含核酸酶的环境中。 意外的是，增加或维持链偏倚，同时必需保持内源RNA干扰的效力，不足以减少细胞生物学测定中的脱靶效应。
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4.发明申请SiRNA Sequence-Independent Modification Formats for Reducing Off-Target Phenotypic Effects in RNAi, and Stabilized Forms Thereof 有权用于减少RNAi中的非目标表型效应的siRNA序列非依赖性修饰形式及其稳定形式公开(公告)号：US20100222414A1
公开(公告)日：2010-09-02
申请号：US12727336
申请日：2010-03-19
IPC分类号： A61K31/7105 , C07H21/00 , C07H21/02 , C12N5/071 , A61P43/00
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1137 , C12N15/1138 , C12N2310/14 , C12N2310/319 , C12N2310/321 , C12N2310/3231 , C12N2310/343 , C12N2320/53 , C12N2310/3521
摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
摘要翻译： 提供了具有修饰的核苷酸的修饰形式用于siRNA。 具有修饰形式的短干扰RNA和本文提供的经修饰的核苷酸减少RNA干扰内源基因的脱靶效应。 证明进一步修饰格式化的siRNA被稳定到富含核酸酶的环境中。 意外的是，增加或维持链偏倚，同时必需保持内源性RNA干扰的效力，不足以减少细胞生物学测定中的脱靶效应。
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5.发明申请Methods of preparation of gene-specific oligonucleotide libraries and uses thereof 审中-公开基因特异性寡核苷酸文库的制备方法及其用途公开(公告)号：US20060051789A1
公开(公告)日：2006-03-09
申请号：US11173100
申请日：2005-07-01
申请人： Sergei Kazakov , Alexander Vlassov , Anne Dallas , Attila Seyhan , Levente Egry , Heini Ilves , Roger Kaspar , Brian Johnston
发明人： Sergei Kazakov , Alexander Vlassov , Anne Dallas , Attila Seyhan , Levente Egry , Heini Ilves , Roger Kaspar , Brian Johnston
CPC分类号： C12N15/1093 , C12P19/34 , C40B40/08 , C40B50/06
摘要： Methods of preparing gene-specific oligonucleotide libraries are disclosed. In one embodiment a double-stranded RNA corresponding to both sense and antisense strands of mRNA is digested by ribonuclease to produce short RNA fragments. In subsequent ligation steps, flanking oligoribonucleotides of defined sequences may be attached to the 3- and 5-ends of each fragment by RNA ligase (such as T4 RNA ligase). The products of ligation can be reverse transcribed and PCR amplified (RT-PCR) using the oligonucleotides attached to the gene-derived sequences as primer-binding sites. Various methods for incorporating libraries into expression vectors allowing expression of either siRNAs or shRNAs are also disclosed.
摘要翻译： 公开了制备基因特异性寡核苷酸文库的方法。 在一个实施方案中，对应于mRNA的有义链和反义链的双链RNA被核糖核酸酶消化以产生短RNA片段。 在随后的连接步骤中，确定序列的侧翼寡核糖核苷酸可以通过RNA连接酶（例如T4 RNA连接酶）连接到每个片段的3和5末端。 连接产物可以使用连接到基因衍生序列的寡核苷酸作为引物结合位点进行逆转录和PCR扩增（RT-PCR）。 还公开了将文库掺入允许表达siRNA或shRNA的表达载体的各种方法。
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公开(公告)号：US09145556B2
公开(公告)日：2015-09-29
申请号：US13085878
申请日：2011-04-13
CPC分类号： C12N15/113 , A61K31/713 , A61K47/50 , C12N15/111 , C12N2310/113 , C12N2310/141 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2310/332 , C12N2310/3515 , C12N2310/3519 , C12N2310/51 , C12N2310/531 , C12N2330/31
摘要： The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.
摘要翻译： 本发明一般涉及通过序列特异性结合抑制靶核酸功能的组合物和方法。 组合物和方法可用于抑制微RNA和其他相对较短的非编码RNA。
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公开(公告)号：US20070149470A1
公开(公告)日：2007-06-28
申请号：US11444901
申请日：2006-06-01
申请人： Roger Kaspar , Heini Ilves , Attila Seyhan , Alexander Vlassov , Brian Johnston
发明人： Roger Kaspar , Heini Ilves , Attila Seyhan , Alexander Vlassov , Brian Johnston
CPC分类号： C12N15/1131 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2770/24211 , C12N2770/36111
摘要： The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.
摘要翻译： 本发明提供了可用于抑制病毒介导的基因表达的小干扰RNA（shRNA或siRNA）的方法，组合物和试剂盒。 本文所述的小干扰RNA可用于治疗HCV感染的方法。 描述了靶向HCV的内部核糖体进入位点（IRES）序列的ShRNA和siRNA构建体。
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