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    Methods of preparation of gene-specific oligonucleotide libraries and uses thereof
    1.
    发明申请
    Methods of preparation of gene-specific oligonucleotide libraries and uses thereof 审中-公开
    基因特异性寡核苷酸文库的制备方法及其用途

    公开(公告)号：US20060051789A1

    公开(公告)日：2006-03-09

    申请号：US11173100

    申请日：2005-07-01

    申请人： Sergei Kazakov ,  Alexander Vlassov ,  Anne Dallas ,  Attila Seyhan ,  Levente Egry ,  Heini Ilves ,  Roger Kaspar ,  Brian Johnston

    发明人： Sergei Kazakov ,  Alexander Vlassov ,  Anne Dallas ,  Attila Seyhan ,  Levente Egry ,  Heini Ilves ,  Roger Kaspar ,  Brian Johnston

    IPC分类号： C40B40/08 ,  C12P19/34

    CPC分类号： C12N15/1093 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    摘要： Methods of preparing gene-specific oligonucleotide libraries are disclosed. In one embodiment a double-stranded RNA corresponding to both sense and antisense strands of mRNA is digested by ribonuclease to produce short RNA fragments. In subsequent ligation steps, flanking oligoribonucleotides of defined sequences may be attached to the 3- and 5-ends of each fragment by RNA ligase (such as T4 RNA ligase). The products of ligation can be reverse transcribed and PCR amplified (RT-PCR) using the oligonucleotides attached to the gene-derived sequences as primer-binding sites. Various methods for incorporating libraries into expression vectors allowing expression of either siRNAs or shRNAs are also disclosed.

    摘要翻译： 公开了制备基因特异性寡核苷酸文库的方法。 在一个实施方案中，对应于mRNA的有义链和反义链的双链RNA被核糖核酸酶消化以产生短RNA片段。 在随后的连接步骤中，确定序列的侧翼寡核糖核苷酸可以通过RNA连接酶（例如T4 RNA连接酶）连接到每个片段的3和5末端。 连接产物可以使用连接到基因衍生序列的寡核苷酸作为引物结合位点进行逆转录和PCR扩增（RT-PCR）。 还公开了将文库掺入允许表达siRNA或shRNA的表达载体的各种方法。

    Polynucleotides capable of target-depedent circularization and topological linkage
    3.
    发明申请
    Polynucleotides capable of target-depedent circularization and topological linkage 审中-公开
    能够靶向环化和拓扑结合的多核苷酸

    公开(公告)号：US20070105108A1

    公开(公告)日：2007-05-10

    申请号：US10561691

    申请日：2004-06-25

    申请人： Sergei Kazakov ,  Anne Dallas ,  Tai-Chih Kuo ,  Brian Johnston

    发明人： Sergei Kazakov ,  Anne Dallas ,  Tai-Chih Kuo ,  Brian Johnston

    IPC分类号： C40B40/08 ,  C40B30/06 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/111 ,  C12N2310/11 ,  C12N2310/122 ,  C12N2310/3519 ,  C12N2310/53 ,  C12N2320/13

    摘要： The invention provides allosterically regulatable polynucleotides capable of target-dependent circularization and topological linkage to a target nucleic acid molecule. Polynucleotides of the invention include a target binding sequence and a regulatory element which prevents circularization in the absence of the target binding. Polynucleotides may include a catalytic domain, allowing circularization to proceed via catalysis when the target binding sequence of the polynucleotide is bound to the target. Topologically linked polynucleotides may be used for detection of target molecules or to inhibit transcription or translation of the target.

    摘要翻译： 本发明提供能够靶向依赖性环化和与靶核酸分子拓扑连接的变构可调节多核苷酸。 本发明的多核苷酸包括靶结合序列和在没有靶结合的情况下防止环化的调节元件。 多核苷酸可以包括催化结构域，当多核苷酸的靶结合序列与靶标结合时，允许通过催化进行环化。 拓扑结合的多核苷酸可用于检测靶分子或抑制靶的转录或翻译。

    Modular and towable farm implement
    5.
    发明授权
    Modular and towable farm implement 有权
    模块化和可拖曳的农具

    公开(公告)号：US08079425B1

    公开(公告)日：2011-12-20

    申请号：US12468371

    申请日：2009-05-19

    申请人： Craig Van Kooten ,  Brian Johnston

    发明人： Craig Van Kooten ,  Brian Johnston

    IPC分类号： E02F3/76 ,  A01B49/02

    CPC分类号： A01B51/04 ,  A01B63/22

    摘要： A towable farm and garden implement trailer which has a modular receiver. The modular receiver is operatively configured to receive implements above or below the frame of the trailer. Implements which may be removably coupled to the underside of the trailer include such implements as a lawn de-thatcher, transport box, box blade, drag harrow, slurry blade, etc. Examples of implements which could be carried and removably coupled to the top side of the trailer frame include such implements as a spreader, tank, carrying platform and other similar implements. In one form, the implement trailer comprises an actuator positioned between the frame and the wheels to allow the wheels to be raised and lowered with respect to the trailer frame.

    摘要翻译： 具有模块化接收器的可拖曳的农场和花园拖车。 模块化接收器可操作地构造成在拖车的框架上方或下方接收工具。 可拆卸地连接到拖车底面的实施例包括诸如草坪去锯机，运输箱，盒式叶片，拖曳耙，浆料叶片等的工具。可以承载并可拆卸地联接到顶部的工具的实例 拖车框架包括诸如吊具，罐，承载平台和其它类似工具的工具。 在一种形式中，工具拖车包括定位在框架和车轮之间的致动器，以允许车轮相对于拖车车架升高和降低。

    Temperature control system to independently maintain separate molten polymer streams at selected temperatures during fiber extrusion
    6.
    发明授权
    Temperature control system to independently maintain separate molten polymer streams at selected temperatures during fiber extrusion 有权
    温度控制系统，用于在纤维挤出过程中独立维持分离的熔融聚合物流

    公开(公告)号：US07252493B1

    公开(公告)日：2007-08-07

    申请号：US10676271

    申请日：2003-10-02

    申请人： Brian Johnston ,  Jeff Haggard ,  Arnold Wilkie ,  Ruey Chern ,  Mark Miller ,  Tony Owen ,  John Quinlivan ,  Ted Mire ,  Brent Soucier ,  Kevin Moschetti

    发明人： Brian Johnston ,  Jeff Haggard ,  Arnold Wilkie ,  Ruey Chern ,  Mark Miller ,  Tony Owen ,  John Quinlivan ,  Ted Mire ,  Brent Soucier ,  Kevin Moschetti

    IPC分类号： D01D1/06 ,  D01D4/02

    CPC分类号： D01D4/06 ,  D01D1/09

    摘要： A temperature control system for use in a fiber extrusion process includes a number of metering pump assemblies including inlets to receive at least two molten polymer streams from a supply source that is connectable to the system. A pump block disposed proximate the metering pump assemblies includes a plurality of flow paths extending within the pump block, where the flow paths are aligned to receive molten polymer flowing from outlets of the metering pump assemblies and to deliver the molten polymer to a spinneret. The flow paths are arranged in flow path sets within the pump block, and each flow path set includes at least one flow path and is spaced a selected distance from the other flow path sets within the pump block so as to facilitate independent control of the temperature of a molten polymer flowing through each flow path set.

    摘要翻译： 用于纤维挤出方法的温度控制系统包括多个计量泵组件，其包括从可连接到系统的供应源接收至少两个熔融聚合物流的入口。 设置在计量泵组件附近的泵块包括在泵块内延伸的多个流动路径，其中流动路径对准以接收从计量泵组件的出口流动的熔融聚合物并将熔融聚合物输送到喷丝头。 流动路径布置在泵块内的流动路径组中，并且每个流动路径组包括至少一个流动路径，并且与泵块内的其它流动路径组间隔开选定的距离，以便于独立控制温度 的熔融聚合物流过每个流动路径组。

    Color motion picture print film
    7.
    发明申请
    Color motion picture print film 审中-公开
    彩色动态影像打印胶卷

    公开(公告)号：US20050147933A1

    公开(公告)日：2005-07-07

    申请号：US10749825

    申请日：2003-12-31

    申请人： Brian Johnston ,  Kathleen Gisser

    发明人： Brian Johnston ,  Kathleen Gisser

    IPC分类号： G03C5/04 ,  G03C7/22 ,  G03C7/30 ,  G03C1/46

    CPC分类号： G03C7/3017 ,  G03C5/04 ,  G03C7/3022 ,  G03C7/3041 ,  G03C2001/03517 ,  G03C2001/03594 ,  G03C2007/3024 ,  G03C2007/3025

    摘要： Processing color motion picture film to yield a dye-only, “silverless” soundtrack enables reduced silver levels to be incorporated into all three color image records of a motion picture print film while still providing a good soundtrack signal in the resulting processed film. A silver halide light sensitive motion picture photographic print element is disclosed comprising a support bearing on one side thereof: a blue color sensitive, yellow dye image-forming record comprising at least one blue-sensitive silver halide emulsion having associated therewith yellow dye-forming coupler; a red color sensitive, cyan dye image-forming record comprising at least one red-sensitive silver halide emulsion having associated therewith cyan dye-forming coupler; and a green color sensitive, magenta dye image-forming record comprising at least one green-sensitive silver halide emulsion having associated therewith magenta dye-forming coupler; wherein each of the silver halide emulsions have an average grain size of less than 1 micrometer and comprise at least 50 mol percent chloride, based on silver, the silver halide emulsions in total comprise from 500-1350 mg/m2 silver, the cyan, magenta and yellow dye-forming couplers are present at levels sufficient to provide visual densities of at least 3.3 when completely consumed, the silver to dye-forming coupler stoichiometric equivalent molar ratio in each of the image-forming records is less than 1.4, and the silver to dye-forming coupler stoichiometric equivalent molar ratio in at least one of the image-forming records is less than 1.0.

    摘要翻译： 处理彩色电影胶片以产生仅染色的“无银”音轨，使得能够将降低的银色电平并入到电影打印胶片的所有三个彩色图像记录中，同时仍然在所得到的处理胶片中提供良好的音带信号。 公开了一种卤化银光敏电影照相印相元件，其包括在其一侧上的支撑轴承：蓝色感光黄色染料成像记录，其包含至少一种蓝色感光卤化银乳剂，其具有与其形成黄色染料的成色剂 ; 红色敏感的青色染料成像记录，其包含至少一种具有与其形成青色染料的成色剂相关联的感红卤化银乳剂; 以及绿色敏感的品红色染料成像记录，其包含至少一种具有与其形成品红染料的成色剂相关联的感绿卤化银乳剂; 其中每个卤化银乳剂具有小于1微米的平均粒度，并且基于银包含至少50摩尔％的氯化物，所述卤化银乳剂总共包含500-1350mg / m 2 / SUP>银，青色，品红色和黄色染料形成成色剂的存在水平足以在完全消耗时提供至少3.3的视觉密度，每种成像记录中银与染料形成的偶联化学计量当量摩尔比 小于1.4，并且至少一个图像形成记录中的银对染料形成的偶联剂化学计量当量摩尔比小于1.0。

    INDUCTION SPLICING OF PHOTOGRAPHIC FILM STRIPS
    8.
    发明申请
    INDUCTION SPLICING OF PHOTOGRAPHIC FILM STRIPS 失效
    摄影胶片感染分离

    公开(公告)号：US20050103430A1

    公开(公告)日：2005-05-19

    申请号：US10716158

    申请日：2003-11-18

    申请人： Thomas Smith ,  Brian Johnston ,  Narasimharao Dontula ,  Luba Kordovski

    发明人： Thomas Smith ,  Brian Johnston ,  Narasimharao Dontula ,  Luba Kordovski

    IPC分类号： G03D15/04 ,  B65H19/00 ,  B65H69/02

    CPC分类号： G03D15/043 ,  Y10T428/19

    摘要： A method is described for splicing together overlapping ends of first and second lengths of photographic film strips of common film strip width, comprising positioning a bonding element between an overlapping end of the first length of photographic film and a corresponding overlapped end of the second length of photographic film, and heating the bonding element to effect an adhesive bond between such film ends, wherein the bonding element comprises an induction heating receptive support and thermoplastic adhesive layers on each side of the support, and wherein the heating of the bonding element is performed by induction heating. The present invention allows for the preparation of photographic film splices, consisting of either homogeneous or dissimilar film bases, using a bonding element and induction heating to provide smooth yet strong splices. In particular, the invention enables successful splicing of acetate support (e.g., cellulose triacetate (CTA)) based films and polyester support (e.g., polyethylene terephthalate (PET)) based films either to themselves or each other. The invention provides a method of forming composite rolls of motion picture film containing different film bases as well as eliminating the need for emulsion skiving, and the use of toxic, flammable film cements when splicing CTA films.

    摘要翻译： 描述了一种用于将共同胶片带宽度的第一和第二长度的照相胶片条的重叠端拼接在一起的方法，包括将结合元件定位在第一长度的照相胶片的重叠端和第二长度的相应重叠端之间 照相胶片，并且加热粘合元件以实现这种胶片端部之间的粘结，其中粘合元件包括感应加热接收支撑体和在支撑体的每一侧上的热塑性粘合剂层，并且其中粘合元件的加热由 感应加热。 本发明允许使用粘合元件和感应加热来制备由均相或异种膜基底组成的照相胶片接合件，以提供平滑而强烈的接合。 特别地，本发明能够成功地将乙酸酯载体（例如，三乙酸纤维素（CTA））基膜和聚酯载体（例如聚对苯二甲酸乙二醇酯（PET））基膜接合到其自身或彼此上。 本发明提供了一种形成含有不同膜基的电影胶片复合辊的方法，并且不需要乳液切割，以及在接合CTA膜时使用有毒的易燃膜水泥。

    Back pack suitcase
    9.
    发明授权
    Back pack suitcase 失效
    背包手提箱

    公开(公告)号：US3995802A

    公开(公告)日：1976-12-07

    申请号：US630533

    申请日：1975-11-10

    申请人： Brian Johnston

    发明人： Brian Johnston

    IPC分类号： A45F3/04

    CPC分类号： A45F3/04

    摘要： A suitcase is provided with shoulder straps in one of its broad sides so that the suitcase can be carried in the manner of a back pack. The straps are retractable into flat engagement with the broad side and the suitcase includes a handle permitting it to be carried in a conventional manner.

    摘要翻译： 一个手提箱在其宽阔的一侧设有肩带，以便手提箱能够以背包的方式携带。 带子可伸缩成与宽侧面的平面接合，并且手提箱包括允许以常规方式承载的手柄。