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371 条记录 (耗时 0.037 秒)

    Method and System for Multiplex Genetic Analysis
    2.
    发明申请
    Method and System for Multiplex Genetic Analysis 有权

    公开(公告)号：US20220154274A1

    公开(公告)日：2022-05-19

    申请号：US17537153

    申请日：2021-11-29

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Eric S. NORDMAN ,  Mark F. OLDHAM ,  Timothy WOUDENBERG

    IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N21/64

    摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.

    Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
    5.
    发明授权
    Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis 有权

    公开(公告)号：US10551345B2

    公开(公告)日：2020-02-04

    申请号：US15595439

    申请日：2017-05-15

    申请人： Applied Biosystems, LLC

    发明人： Aldrich N. K. Lau

    IPC分类号： G01N27/453 ,  G01N27/447 ,  C08F20/54

    摘要： The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis.

    Information System for Biological and Life Sciences Research
    6.
    发明申请
    Information System for Biological and Life Sciences Research 审中-公开

    公开(公告)号：US20190385103A1

    公开(公告)日：2019-12-19

    申请号：US16451217

    申请日：2019-06-25

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Ramin Cyrus ,  Matteo Di Tommaso ,  Anthony Kerlavage ,  Charles Lawrence

    IPC分类号： G06Q10/06 ,  G06Q10/08 ,  G16B50/00 ,  G06Q30/06

    摘要： An online life science research environment and virtual community with a focus on design and analysis of biological experiments includes a life sciences laboratory system employing at least one networked computer system that defines a virtual research environment. Users access the system through a portal associated with the networked computer system(s). The virtual research environment has a data coupling mechanism by which the user designates a set of user-specified data for bioinformatics processing. A processor(s) associated with the networked computer system(s) performs bioinformatics services upon the user-specified data. In one embodiment, the data coupling mechanism enables transfer of the user-specified data to a memory space that is mediated or accessed by the processor performing the bioinformatics processing. Users may thus exploit bioinformatics processing resources that are not deployed on users' local computer environments, and to store and organize information relating to life sciences research in a secure, online workspace.

    Robust Detection Of Variablility In Multiple Sets Of Data
    7.
    发明申请
    Robust Detection Of Variablility In Multiple Sets Of Data 审中-公开

    公开(公告)号：US20190228053A1

    公开(公告)日：2019-07-25

    申请号：US16259994

    申请日：2019-01-28

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Chengyong YANG ,  David WOO

    IPC分类号： G06F17/18 ,  G01N21/64 ,  G01D1/14

    摘要： The present teachings comprise systems and methods for calibrating the background or baseline signal in a PCR or other reaction. The background signal derived from detected emissions of sample wells can be subjected to a normalized statistical metric, and be compared to a threshold or other standard to discard outlier cycles or other extraneous data. According to various embodiments, a relative standard deviation (relativeSTD) for the background component can be generated by dividing the standard deviation by the median of differences across all wells, where the difference is defined as the difference between maximum and minimum pixel values of a well. The relativeSTD as a metric is not sensitive to machine-dependent variations in absolute signal output that can be caused by different gain settings, different LED draw currents, different optical paths, or other instrumental variations. More accurate background characterization can be achieved.

    Reagents, methods, and libraries for bead-based sequencing
    9.
    发明授权
    Reagents, methods, and libraries for bead-based sequencing 有权

    公开(公告)号：US10323277B2

    公开(公告)日：2019-06-18

    申请号：US15291982

    申请日：2016-10-12

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Kevin McKernan ,  Alan Blanchard ,  Lev Kotler ,  Gina Costa

    IPC分类号： C12P19/34 ,  C12Q1/6874 ,  C12Q1/68 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6869

    摘要： The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides.