Method for selecting a high affinity, low molecular weight displacer for oligonucleotide purification
    2.
    发明授权
    Method for selecting a high affinity, low molecular weight displacer for oligonucleotide purification 失效
    选择高亲和力,低分子量置换剂用于寡核苷酸纯化的方法

    公开(公告)号:US06828436B2

    公开(公告)日:2004-12-07

    申请号:US10146266

    申请日:2002-05-15

    IPC分类号: C07H2100

    CPC分类号: C12N15/101 B01D15/422

    摘要: A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.

    摘要翻译: 公开了使用高亲和力,低分子量(小于约10000Da)置换剂在阴离子交换介质上通过置换色谱法纯化寡核苷酸的方法。 提供了高亲和性,低分子量阴离子置换剂的几个实例,包括其上连接有磺酸基团的多环芳族化合物。 该技术用于高分辨率分离寡核苷酸的功效证明了工业混合物。

    Method for determining twist potential in wood
    4.
    发明授权
    Method for determining twist potential in wood 有权
    确定木材扭转电位的方法

    公开(公告)号:US06293152B1

    公开(公告)日:2001-09-25

    申请号:US09389676

    申请日:1999-09-02

    IPC分类号: G01N3346

    摘要: A method for determining twist potential of wood is described. One embodiment of the method comprises nondestructively obtaining a grain angle of wood, such as trees or lumber, and then determining twist potential of the wood based on the grain angle. Where the wood comprises lumber, grain angle determinations typically are made on at least one major surface of the lumber. The method typically comprises determining grain angle at at least two measuring locations separated by a predetermined distance, such as at substantially one-foot intervals along the board. The method also can further comprise obtaining at least one fiber dive angle. Particular embodiments of the present invention determine grain angle using infrared radiation, microwave radiation, light energy (such as by using a laser), electricity, ultrasound energy, and combinations thereof. Working embodiments of the method used light energy and ultrasound energy to determine grain angle. For example, a first transmission speed of an ultrasound pulse through the board was determined along a first path and along a second path. Grain angle was then determined by comparing the first and second transmission speeds. Plural grain angles also can be determined using this ultrasound method. Twist potential can be correlated to empirically determined twist angle. The method of the present invention provides can determine twist potential that correlates with empirically determined twist potential with an R2 value of at least 0.50.

    摘要翻译: 描述了确定木材扭转电位的方法。 该方法的一个实施例包括非破坏性地获得木材(例如树木或木材)的晶粒角度,然后基于晶粒角度确定木材的扭转电位。 在木材包括木材的情况下,通常在木材的至少一个主表面上进行晶粒角度测定。 该方法通常包括在至少两个测量位置处确定分隔一预定距离的晶粒角度,例如沿板的基本上一英尺的间隔。 该方法还可以进一步包括获得至少一个纤维潜水角。 本发明的具体实施例使用红外辐射,微波辐射,光能(例如通过使用激光),电力,超声能量及其组合来确定晶粒角度。 该方法的工作实施例使用光能和超声能量来确定晶粒角度。 例如,沿着第一路径和第二路径确定通过板的超声脉冲的第一传输速度。 然后通过比较第一和第二传输速度来确定晶粒角度。 也可以使用该超声波方法来确定多个晶粒角度。 扭转电位可以与经验确定的扭转角度相关。 本发明的方法可以确定与经验确定的扭转电位相关的扭转电位,R2值至少为0.50。

    Light with clamp that fits into a headband
    7.
    发明授权
    Light with clamp that fits into a headband 失效
    带夹子的灯适合头带

    公开(公告)号:US06575587B2

    公开(公告)日:2003-06-10

    申请号:US09915627

    申请日:2001-07-26

    IPC分类号: F21V21084

    摘要: A light that is mounted on a clamp. The clamp is configured to be attached to different locations on a user's body, and is sufficiently sturdy so that it can support the light in an illumination direction that is fixed relative to the user's body. The light is connected by a cord to a power supply, such as a battery pack or an AC adapter. If a battery pack is used, the battery pack may also include a mechanism, such as a clip, for attaching the battery pack to the clothing or belt of a user. A headband is provided to which the clamp may be attached. The light is pivotally attached to the clamp so the light may be pivoted to illuminate in a desired direction. In addition, the light is rotatably mounted on the clamp arm, permitting the light to illuminate in a wide variety of different angles.

    摘要翻译: 安装在夹具上的灯。 夹具被配置为附接到使用者身体上的不同位置,并且足够坚固,使得其能够以相对于使用者身体固定的照明方向支撑光。 光线通过电缆连接到电源,例如电池组或AC适配器。 如果使用电池组,则电池组还可以包括用于将电池组附接到使用者的衣服或皮带上的机构,例如夹子。 提供头带,夹具可附接到头带上。 光可枢转地附接到夹具,使得光可以枢转以沿期望的方向照亮。 此外,光可旋转地安装在夹持臂上,允许光以各种不同的角度照亮。