摘要:
A method for purification of proteins by displacement chromatography in hydrophobic interaction and reversed phase chromatographic systems uses low molecular weight (less than about 10,000) surface-active compounds as displacers. Examples of effective displacers are benzethonium chloride, benzyltributylammonium chloride, and tetrahexylammonium chloride.
摘要:
A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
摘要:
A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
摘要:
A method for determining twist potential of wood is described. One embodiment of the method comprises nondestructively obtaining a grain angle of wood, such as trees or lumber, and then determining twist potential of the wood based on the grain angle. Where the wood comprises lumber, grain angle determinations typically are made on at least one major surface of the lumber. The method typically comprises determining grain angle at at least two measuring locations separated by a predetermined distance, such as at substantially one-foot intervals along the board. The method also can further comprise obtaining at least one fiber dive angle. Particular embodiments of the present invention determine grain angle using infrared radiation, microwave radiation, light energy (such as by using a laser), electricity, ultrasound energy, and combinations thereof. Working embodiments of the method used light energy and ultrasound energy to determine grain angle. For example, a first transmission speed of an ultrasound pulse through the board was determined along a first path and along a second path. Grain angle was then determined by comparing the first and second transmission speeds. Plural grain angles also can be determined using this ultrasound method. Twist potential can be correlated to empirically determined twist angle. The method of the present invention provides can determine twist potential that correlates with empirically determined twist potential with an R2 value of at least 0.50.
摘要:
Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a group in place of at least one hydrogen atom of at least one hydroxyl group of the carbohydrate compound. In these compounds R1 is an alkyl group or an azaalkyl group, and R2 is a primary or secondary amino group.
摘要:
A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:
摘要:
A light that is mounted on a clamp. The clamp is configured to be attached to different locations on a user's body, and is sufficiently sturdy so that it can support the light in an illumination direction that is fixed relative to the user's body. The light is connected by a cord to a power supply, such as a battery pack or an AC adapter. If a battery pack is used, the battery pack may also include a mechanism, such as a clip, for attaching the battery pack to the clothing or belt of a user. A headband is provided to which the clamp may be attached. The light is pivotally attached to the clamp so the light may be pivoted to illuminate in a desired direction. In addition, the light is rotatably mounted on the clamp arm, permitting the light to illuminate in a wide variety of different angles.
摘要:
Disclosed herein are polymers that can be made cationic and used to deliver a substance into a cell. Also disclosed are pharmaceutical compositions comprising the polymers and methods of using the polymers.
摘要:
A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R.sup.1 is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.