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1.发明授权Low molecular weight displacers for protein purification in hydrophobic interaction and reversed phase chromatographic systems 有权用于疏水相互作用和反相色谱系统中蛋白质纯化的低分子量置换剂公开(公告)号：US06239262B1
公开(公告)日：2001-05-29
申请号：US09223093
申请日：1998-12-30
IPC分类号： C07K114
CPC分类号： C12N9/2462 , B01D15/325 , B01D15/327 , B01D15/422 , C07K14/475 , C07K14/76
摘要： A method for purification of proteins by displacement chromatography in hydrophobic interaction and reversed phase chromatographic systems uses low molecular weight (less than about 10,000) surface-active compounds as displacers. Examples of effective displacers are benzethonium chloride, benzyltributylammonium chloride, and tetrahexylammonium chloride.
摘要翻译： 在疏水相互作用和反相色谱系统中通过置换色谱法纯化蛋白质的方法使用低分子量（少于约10,000）表面活性化合物作为置换剂。 有效置换剂的实例是苄索氯铵，苄基三丁基氯化铵和四己基氯化铵。
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公开(公告)号：US06433146B1
公开(公告)日：2002-08-13
申请号：US09313690
申请日：1999-05-18
申请人： Munir Cheryan
发明人： Munir Cheryan
IPC分类号： C07K114
CPC分类号： A23J1/142 , A23V2002/00 , C11B1/10 , C11B1/108 , A23V2300/34
摘要： The invention is a corn product removal process that successfully extracts oil and zein from dry-milled corn. Oils and zein are extracted from corn using ethanol. Corn solids are separated from the ethanol, oil and zein mixture produced in the step of extracting. Thereafter, the ethanol, oil and zein mixture are membrane filtered to restrain zein from the mixture and pass an oil and ethanol mixture. At least one of zein or oil is then selected to be separated for an output corn product.
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3.发明授权公开(公告)号：US06335430B1
公开(公告)日：2002-01-01
申请号：US09722417
申请日：2000-11-28
申请人： Magnus Qvist
发明人： Magnus Qvist
IPC分类号： C07K114
CPC分类号： C07K14/43504 , Y10S530/857
摘要： The invention relates to a process of producing MAP-products from mussel feet. The process is characterised by extracting the mussel feet in a weakly acid aqueous solution containing, for instance, 1-10 percent by weight of a weak acid and 0.5-3 percent by weight of perchloric acid, whereafter the proteins in the aqueous solution are precipitated by adding inorganic or organic salts and separated from the system, subsequent to having extracted the solid substances.
摘要翻译： 本发明涉及从贻贝脚生产MAP-产品的方法。 该方法的特征在于在含有例如1-10重量％的弱酸和0.5-3重量％的高氯酸的弱酸水溶液中提取贻贝脚，然后水溶液中的蛋白质沉淀 在提取固体物质之后，加入无机或有机盐并与体系分离。
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公开(公告)号：US06291654B1
公开(公告)日：2001-09-18
申请号：US09308022
申请日：1999-05-12
申请人： Margaret K. Hostetter , Qi Cheng
发明人： Margaret K. Hostetter , Qi Cheng
IPC分类号： C07K114
CPC分类号： C07K14/3156 , A61K38/00 , A61K39/00 , C07K14/70539 , C07K16/1275
摘要： This invention relates to the identification of a human complement C3 binding protein from Streptococcus pneumoniae and to its sequence and to methods for its purification and use. The protein binds but does not degrade or cleave C3 and is implicated in S. pneumoniae virulence. The protein is recognized by antibodies produced by humans recovering from pneumococcal infection.
摘要翻译： 本发明涉及鉴定来自肺炎链球菌的人补体C3结合蛋白及其序列及其纯化和使用方法。 蛋白质结合但不降解或切割C3，并且涉及肺炎链球菌毒力。 蛋白质被从肺炎球菌感染中恢复的人产生的抗体识别。
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公开(公告)号：US06767994B1
公开(公告)日：2004-07-27
申请号：US09571683
申请日：2000-05-16
申请人： Hans Ageland , Lena Nyström , Josefine Persson , Folke Tjerneld
发明人： Hans Ageland , Lena Nyström , Josefine Persson , Folke Tjerneld
IPC分类号： C07K114
CPC分类号： C07K14/775 , A61K38/00
摘要： The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step. The present invention further relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first polymeric material and a surfactant, said first polymeric material and surfactant being immiscible in the primary aqueous solution obtained after mixing with water. ApoA or ApoE produced by the inventive process can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.
摘要翻译： 本发明涉及一种纯化疏水性或两亲性化合物的方法，首先将含有疏水性或两亲性化合物的原料与第一聚合物材料，水和至少一种第二聚合物材料和表面活性剂混合，其中第一 聚合物材料和第二聚合物材料和/或表面活性剂在所得初级水溶液中是不混溶的。 该方法还包括将初级水溶液保持一段时间，足以基本分离所形成的相，然后除去含有疏水性或两亲性化合物和第二聚合物材料和/或表面活性剂的主要部分的相。 将第二聚合物材料和/或表面活性剂与疏水性或两亲性化合物分离，随后循环至初始混合步骤。 本发明还涉及用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一聚合物材料和表面活性剂，所述第一聚合物材料和表面活性剂在获得的主要水溶液中是不混溶的 与水混合后。 本发明方法产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。
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公开(公告)号：US06559284B1
公开(公告)日：2003-05-06
申请号：US08924994
申请日：1997-09-05
申请人： Hans Ageland , Lena Nyström , Josefine Persson , Folke Tjerneld
发明人： Hans Ageland , Lena Nyström , Josefine Persson , Folke Tjerneld
IPC分类号： C07K114
CPC分类号： C07K14/775
摘要： The present invention relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first and a second polymeric material, wherein the first and second polymeric material are immiscible in the primary aqueous solution, and wherein the second polymeric material is amphiphilic and water soluble. The invention further relates to a process for purifying ApoA or ApoE, or variants or mixtures thereof, by first mixing ApoA or ApoE, the composition containing a first and second polymeric material and water. The resulting primary aqueous solution is maintained for a period of time sufficient for essentially separating the phases formed, and removing the phase containing the second polymeric material and the main portion of ApoA or ApoE. Subsequently, the second polymeric material is separated from ApoA or ApoE. The thus produced ApoA or ApoE can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.
摘要翻译： 本发明涉及一种用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一和第二聚合物材料，其中第一和第二聚合物材料在初级水溶液中是不混溶的， 并且其中所述第二聚合物材料是两亲性和水溶性的。 本发明还涉及通过首先混合ApoA或ApoE，所述组合物含有第一和第二聚合物材料和水，来纯化ApoA或ApoE或其变体或混合物的方法。 将得到的初级水溶液保持足够长的时间基本上分离所形成的相，并除去含有第二聚合物材料的相和ApoA或ApoE的主要部分。 随后，将第二聚合物材料与ApoA或ApoE分离。 由此产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。
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公开(公告)号：US06555661B1
公开(公告)日：2003-04-29
申请号：US09486417
申请日：2000-02-25
IPC分类号： C07K114
摘要： High purity protein A is recovered from protein A preparations by passing a protein A preparation over an ion exchange column so that protein A will bind to a cation exchange resin. The loaded column is washed with a wash buffer solution having about the same pH as the protein A preparation and an increased mineral salt concentration to elute certain impurities but not the protein A. The protein A is eluted from the washed column by eluting the column with an elution buffer solution having a pH higher than the wash buffer solution and mineral salt concentration substantially no higher than the wash buffer solution. The protein A can be easily recovered from protein A preparations that do not use any human or animal components. The protein A purified by the invention is suitable for all common uses of protein A, but is especially well-suited for human therapeutic applications.
摘要翻译： 通过将蛋白A制剂通过离子交换柱从蛋白A制备中回收高纯度蛋白A，使蛋白A与阳离子交换树脂结合。 负载的柱用与蛋白A制备具有相同pH的洗涤缓冲溶液洗涤，并且增加的矿物质盐浓度以洗脱某些杂质而不是蛋白质A.蛋白质A通过用洗脱柱洗脱， 洗脱缓冲溶液，其pH高于洗涤缓冲溶液，矿物盐浓度基本上不高于洗涤缓冲溶液。 蛋白A可以从不使用任何人或动物成分的蛋白A制剂中容易地回收。 本发明纯化的蛋白A适用于蛋白A的所有常见用途，但特别适用于人类治疗应用。
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公开(公告)号：US06448374B1
公开(公告)日：2002-09-10
申请号：US08137086
申请日：1994-03-04
IPC分类号： C07K114
CPC分类号： C07K14/4732 , A61K38/00
摘要： A method for the preparation of selected phosphopeptides having anticariogenic and other activities, comprising the steps of completely digesting a soluble monovalent cation salt of casein in solution with a proteolytic enzyme, adding a mineral acid to the solution to adjust the pH to about 4.7, removing any precipitate produced, adding CaCl2 to a level of about 1.0% w/v to cause aggregation of at least the selected phosphopeptides in said digested solution, separating the aggregated phosphopeptides from the solution through a filter having a molecular weight exclusion limit lying substantially within the range 10,000 to 20,000 while passing the bulk of the remaining phosphopeptides and solution, diafiltering the separated phosphopeptides with water through a filter and concentrating and drying the retentate.
摘要翻译： 一种制备具有抗焦虑和其他活性的选择性磷酸肽的方法，包括以下步骤：用蛋白水解酶在溶液中完全消化酪蛋白的可溶性一价阳离子盐，向溶液中加入无机酸以将pH调节至约4.7，除去 产生任何沉淀物，将CaCl 2加入到约1.0％w / v的水平，以使至少所述消化溶液中选择的磷酸肽聚集，通过具有分子量排除极限基本上在 范围为10,000至20,000，同时通过大部分剩余的磷酸肽和溶液，通过过滤器将水分离过滤分离的磷酸肽并浓缩和干燥滞留物。
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公开(公告)号：US06420532B1
公开(公告)日：2002-07-16
申请号：US09407367
申请日：1999-09-29
IPC分类号： C07K114
CPC分类号： C07K14/70571 , A01K2217/05 , A61K38/00 , C12N2799/026
摘要： This invention provides isolated nucleic acid molecules encoding Y2 receptors, an isolated, purified Y2 receptor protein, vectors comprising isolated nucleic acid molecules encoding Y2 receptors, mammalian, insect, bacterial and yeast cells comprising such vectors, antibodies directed to the Y2 receptors, nucleic acid probes useful for detecting nucleic acid encoding Y2 receptors, antisense oligonucleotides complementary to unique sequences of a nucleic acid molecule which encodes a Y2 receptor, pharmaceutical compounds related to the Y2 receptors, and nonhuman transgenic animals which express nucleic acid encoding a normal or mutant Y2 receptor. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and methods of treatment involving Y2 receptors.
摘要翻译： 本发明提供编码Y2受体的分离的核酸分子，分离的，纯化的Y2受体蛋白，包含编码Y2受体的分离的核酸分子，包含这种载体的哺乳动物，昆虫，细菌和酵母细胞的载体，针对Y2受体的抗体，核酸 可用于检测编码Y2受体的核酸的探针，与编码Y2受体的核酸分子的唯一序列互补的反义寡核苷酸，与Y2受体相关的药物化合物和表达编码正常或突变体Y2受体的核酸的非人转基因动物 。 本发明还提供了确定配体结合，检测表达，药物筛选和涉及Y2受体的治疗方法的方法。
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公开(公告)号：US06268487B1
公开(公告)日：2001-07-31
申请号：US08648235
申请日：1996-05-13
IPC分类号： C07K114
CPC分类号： C07K1/36 , A23J1/20 , Y10S530/832
摘要： A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals which have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.
摘要翻译： 公开了一种从牛奶中分离可溶性牛奶成分的方法。 该方法涉及使用横跨膜的切向流过滤以形成渗余物和渗透物，将渗透物与原始乳样组合，并重复该过程直到牛奶已被充分纯化。 优选地，将牛奶与螯合剂如EDTA混合以提高净化效率。 该方法有利地用于已经被遗传改变以在其牛奶中表达外源蛋白质（例如治疗性蛋白质）的转基因动物的乳。
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