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公开(公告)号:US20110183865A1
公开(公告)日:2011-07-28
申请号:US13076803
申请日:2011-03-31
申请人: AKIKO SUGIO , SHINOBU TAKAGI
发明人: AKIKO SUGIO , SHINOBU TAKAGI
IPC分类号: C12N9/02 , C07H21/04 , C12N15/63 , C07H21/00 , C40B30/04 , C12P7/64 , C12N1/19 , C11D3/386 , A21D10/00 , A21D8/04
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.
摘要翻译: 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序,并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。
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公开(公告)号:US07264954B2
公开(公告)日:2007-09-04
申请号:US10473533
申请日:2002-04-18
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The present disclosure relates to an isolated lipoxygenase which is: a) a polypeptide encoded by the DNA sequence cloned into plasmid pUC19 present in Escherichia coli deposited as DSM 14139; b) a polypeptide having an amino acid sequence of the mature peptide shown in SEQ ID NO: 1; c) a polypeptide which has at least 80% homology with the polypeptide of a) or b); or d) a polypeptide encoded by DNA that hybridizes under high stringency conditions to a complementary strand of: i) the DNA sequence cloned into plasmid pUC19 present in Escherichia coli deposited as DSM 14139; or ii) the DNA sequence of SEQ ID NO: 1 encoding the mature polypeptide.
摘要翻译: 本公开涉及分离的脂氧合酶,其是:a)由克隆到以DSM14139保藏的大肠杆菌中存在的质粒pUC19中的DNA序列编码的多肽; b)具有SEQ ID NO:1所示的成熟肽的氨基酸序列的多肽; c)与a)或b)的多肽具有至少80%同源性的多肽; 或d)由高度严格条件下与下列互补链杂交的由DNA编码的多肽:i)克隆到以DSM14139保藏的大肠杆菌中存在的质粒pUC19中的DNA序列; 或ii)编码成熟多肽的SEQ ID NO:1的DNA序列。
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公开(公告)号:US07456001B2
公开(公告)日:2008-11-25
申请号:US10362776
申请日:2001-09-05
申请人: Soren Christensen , Akiko Sugio , Shinobu Takagi , Lars Ostergaard , Ernst Oliw
发明人: Soren Christensen , Akiko Sugio , Shinobu Takagi , Lars Ostergaard , Ernst Oliw
IPC分类号: C12N9/02 , C12N15/00 , C12N1/20 , C12P21/04 , A21D10/00 , C11D3/02 , C07H21/04 , C12Q1/00 , C12Q1/68
CPC分类号: C12N9/0069
摘要: The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25% identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.
摘要翻译: 本发明提供具有脂氧合酶活性的微生物蛋白质的序列信息和通过重组DNA技术生产该蛋白质的方法。 更具体地说,本发明人已经分离出来自禾谷镰刀菌的编码脂氧合酶的基因,将其克隆到大肠杆菌菌株中并对其进行测序。 比较显示与已知的脂氧合酶序列具有小于25%的同一性,最接近的是人15S li-加氧酶。 本发明人重组表达了脂氧合酶,发现重组脂氧合酶被糖基化。
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公开(公告)号:US20060154842A1
公开(公告)日:2006-07-13
申请号:US10473533
申请日:2002-04-18
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The inventors have found a novel fungal lipoxygenase from Magnaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotide probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.
摘要翻译: 本发明人发现了一种来自Magnaporthe salvinii的新型真菌脂氧合酶,并确定了其序列。 他们对基因进行了测序,并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。
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公开(公告)号:US08263376B2
公开(公告)日:2012-09-11
申请号:US13076803
申请日:2011-03-31
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.
摘要翻译: 本公开涉及分离的脂氧合酶。 本公开的脂氧合酶包括与SEQ ID NO:2所示的成熟肽具有至少80%的序列同源性的多肽。本公开的脂氧合酶适合用于烘烤和洗涤剂组合物中。
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公开(公告)号:US20070292581A1
公开(公告)日:2007-12-20
申请号:US11832472
申请日:2007-08-01
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
IPC分类号: A23L1/277 , A21D2/08 , C07H21/04 , C09B67/00 , C12N1/15 , C12N15/00 , C12N9/02 , C12P21/00 , C12P7/64
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.
摘要翻译: 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序,并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。
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