利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

39 条记录 (耗时 0.018 秒)

    Lipoxygenase
    1.
    发明授权
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号：US07456001B2

    公开(公告)日：2008-11-25

    申请号：US10362776

    申请日：2001-09-05

    申请人： Soren Christensen ,  Akiko Sugio ,  Shinobu Takagi ,  Lars Ostergaard ,  Ernst Oliw

    发明人： Soren Christensen ,  Akiko Sugio ,  Shinobu Takagi ,  Lars Ostergaard ,  Ernst Oliw

    IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C12P21/04 ,  A21D10/00 ,  C11D3/02 ,  C07H21/04 ,  C12Q1/00 ,  C12Q1/68

    CPC分类号： C12N9/0069

    摘要： The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25% identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.

    摘要翻译： 本发明提供具有脂氧合酶活性的微生物蛋白质的序列信息和通过重组DNA技术生产该蛋白质的方法。 更具体地说，本发明人已经分离出来自禾谷镰刀菌的编码脂氧合酶的基因，将其克隆到大肠杆菌菌株中并对其进行测序。 比较显示与已知的脂氧合酶序列具有小于25％的同一性，最接近的是人15S li-加氧酶。 本发明人重组表达了脂氧合酶，发现重组脂氧合酶被糖基化。

    Lipoxygenase
    2.
    发明授权
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号：US08263376B2

    公开(公告)日：2012-09-11

    申请号：US13076803

    申请日：2011-03-31

    申请人： Akiko Sugio ,  Shinobu Takagi

    发明人： Akiko Sugio ,  Shinobu Takagi

    IPC分类号： C12N9/02 ,  C12P2/06 ,  C11D3/386

    CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

    摘要： The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.

    摘要翻译： 本公开涉及分离的脂氧合酶。 本公开的脂氧合酶包括与SEQ ID NO：2所示的成熟肽具有至少80％的序列同源性的多肽。本公开的脂氧合酶适合用于烘烤和洗涤剂组合物中。

    Lipoxygenase
    3.
    发明申请
    Lipoxygenase 审中-公开
    脂氧合酶

    公开(公告)号：US20070292581A1

    公开(公告)日：2007-12-20

    申请号：US11832472

    申请日：2007-08-01

    申请人： Akiko Sugio ,  Shinobu Takagi

    发明人： Akiko Sugio ,  Shinobu Takagi

    IPC分类号： A23L1/277 ,  A21D2/08 ,  C07H21/04 ,  C09B67/00 ,  C12N1/15 ,  C12N15/00 ,  C12N9/02 ,  C12P21/00 ,  C12P7/64

    CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

    摘要： The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.

    摘要翻译： 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序，并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。

    Lipoxygenase
    4.
    发明授权
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号：US07264954B2

    公开(公告)日：2007-09-04

    申请号：US10473533

    申请日：2002-04-18

    申请人： Akiko Sugio ,  Shinobu Takagi

    发明人： Akiko Sugio ,  Shinobu Takagi

    IPC分类号： C12N9/02 ,  C12P21/06 ,  C12H21/04 ,  C11D3/386

    CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

    摘要： The present disclosure relates to an isolated lipoxygenase which is: a) a polypeptide encoded by the DNA sequence cloned into plasmid pUC19 present in Escherichia coli deposited as DSM 14139; b) a polypeptide having an amino acid sequence of the mature peptide shown in SEQ ID NO: 1; c) a polypeptide which has at least 80% homology with the polypeptide of a) or b); or d) a polypeptide encoded by DNA that hybridizes under high stringency conditions to a complementary strand of: i) the DNA sequence cloned into plasmid pUC19 present in Escherichia coli deposited as DSM 14139; or ii) the DNA sequence of SEQ ID NO: 1 encoding the mature polypeptide.

    摘要翻译： 本公开涉及分离的脂氧合酶，其是：a）由克隆到以DSM14139保藏的大肠杆菌中存在的质粒pUC19中的DNA序列编码的多肽; b）具有SEQ ID NO：1所示的成熟肽的氨基酸序列的多肽; c）与a）或b）的多肽具有至少80％同源性的多肽; 或d）由高度严格条件下与下列互补链杂交的由DNA编码的多肽：i）克隆到以DSM14139保藏的大肠杆菌中存在的质粒pUC19中的DNA序列; 或ii）编码成熟多肽的SEQ ID NO：1的DNA序列。

    Lipoxygenase
    5.
    发明申请
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号：US20060154842A1

    公开(公告)日：2006-07-13

    申请号：US10473533

    申请日：2002-04-18

    申请人： Akiko Sugio ,  Shinobu Takagi

    发明人： Akiko Sugio ,  Shinobu Takagi

    IPC分类号： C11D3/386 ,  A21D8/02 ,  C07H21/04 ,  C12P21/06 ,  C12N9/02

    CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

    摘要： The inventors have found a novel fungal lipoxygenase from Magnaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotide probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.

    摘要翻译： 本发明人发现了一种来自Magnaporthe salvinii的新型真菌脂氧合酶，并确定了其序列。 他们对基因进行了测序，并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。

    Method for producing RFeB-based magnet
    6.
    发明授权
    Method for producing RFeB-based magnet 有权
    制造基于RFeB的磁体的方法

    公开(公告)号：US09368277B2

    公开(公告)日：2016-06-14

    申请号：US14492444

    申请日：2014-09-22

    申请人： Shinobu Takagi

    发明人： Shinobu Takagi

    IPC分类号： B05D5/00 ,  H01F41/16 ,  H01F1/053 ,  H01F1/055 ,  H01F41/02

    CPC分类号： H01F41/16 ,  B05D5/00 ,  H01F1/0536 ,  H01F1/0551 ,  H01F41/0293

    摘要： Provided is a method for producing an RFeB-based magnet, the method including: disposing a nozzle so as to be opposed to an attachment surface of a base material that is a sintered magnet or hot-plastic worked magnet composed of an RFeB-based magnet containing a light rare earth element RL that is at least one element selected from the group consisting of Nd and Pr, Fe, and B; ejecting a mixture, from the nozzle, obtained by mixing an organic solvent and an RH-containing powder containing a heavy rare earth element RH that is at least one element selected from the group consisting of Dy, Tb and Ho so as to attach the mixture to the attachment surface; and heating the base material together with the mixture.

    摘要翻译： 本发明提供一种RFeB系磁体的制造方法，其特征在于，包括：将喷嘴配置成与由烧结磁体构成的基材的配合面或由RFeB系磁铁构成的热塑性加工磁铁 含有选自Nd和Pr，Fe和B中的至少一种元素的轻稀土元素RL; 喷射通过混合有机溶剂和含有选自Dy，Tb和Ho中的至少一种元素的重稀土元素RH的含RH粉末得到的混合物，以便将混合物 到附件表面; 并与混合物一起加热基材。

    Polynucleotides having leader sequence function
    9.
    发明授权
    Polynucleotides having leader sequence function 有权
    具有前导序列功能的多核苷酸

    公开(公告)号：US09222095B2

    公开(公告)日：2015-12-29

    申请号：US13704856

    申请日：2011-06-23

    申请人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi

    发明人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi

    IPC分类号： C12N15/80 ,  C07K14/195

    CPC分类号： C12N15/80 ,  C07K14/195

    摘要： The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.

    摘要翻译： 本发明涉及具有前导序列功能的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及使用多核苷酸产生多肽的方法。