公开(公告)号：US4345472A

公开(公告)日：1982-08-24

申请号：US158821

申请日：1980-06-12

申请人： Yoshihumi Hara ,  Saburo Kubota ,  Akira Ihara ,  Masaru Kishimoto ,  Masaki Suzuki

发明人： Yoshihumi Hara ,  Saburo Kubota ,  Akira Ihara ,  Masaru Kishimoto ,  Masaki Suzuki

IPC分类号： G01M1/14 ,  G01M1/08 ,  G01M1/20 ,  G01M1/22

CPC分类号： G01M1/20 ,  G01M1/225

摘要： Mechanical vibration of a rotating test body is translated into a periodic signal which represents a combined vector of the unbalance means of the rotating body and the mechanism that rotates it. The periodic signal is divided into two orthogonal vector components. The magnitude of the two vector components are converted into digital signals and applied to a computer. Unknown values of four constants are determined by a preliminary test having three successive stages. In the first and second stages the test body is rotated to register the digital signals; in the second state the angular position of the test body is displaced a predetermined amount with respect to its first stage position. In the third stage of the test, a trial dead weight of a known mass is mounted on the test body and rotated to register the digital signals. The computer operates on the registered digital signals to determine the four constants in accordance with a set of equations. After the preliminary test, the data registered in the first stage of the preliminary test is substituted into another set of equations using the four constants to determine the dynamic unblance mass and its angular location.

摘要翻译： 旋转测试体的机械振动被转换成周期信号，其表示旋转体的不平衡装置和旋转体的旋转机构的组合矢量。 周期信号被分成两个正交矢量分量。 两个矢量分量的大小被转换为数字信号并应用于计算机。 四个常数的未知值通过具有三个连续阶段的初步测试来确定。 在第一和第二阶段，测试体旋转以注册数字信号; 在第二状态下，测试体的角位置相对于其第一阶段位置移位预定量。 在测试的第三阶段，将已知质量的试验自重安装在测试体上并旋转以注册数字信号。 计算机对注册的数字信号进行操作，以根据一组方程确定四个常数。 在初步测试之后，使用四个常数将初步测试的第一阶段中记录的数据代入另一组方程中，以确定动态不透明质量及其角位置。

公开(公告)号：US20050032201A1

公开(公告)日：2005-02-10

申请号：US10936937

申请日：2004-09-09

申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  C12M1/34

CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are present in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的存在量，即使从固相中回收的细胞与固相相结合， 聚合物，从液相中回收的细胞不与聚合物结合固相。

公开(公告)号：US4477159A

公开(公告)日：1984-10-16

申请号：US318593

申请日：1981-11-05

申请人： Katsuyoshi Mizuno ,  Akira Ihara

发明人： Katsuyoshi Mizuno ,  Akira Ihara

IPC分类号： A61B17/00 ,  A61B19/00 ,  A61F9/00 ,  A61F9/008 ,  A61B3/10

CPC分类号： A61F9/008 ,  A61F9/00821 ,  A61B2017/00973 ,  A61B2090/502 ,  A61F2009/0035 ,  A61F2009/00863

摘要： A photocoagulator of the type having a light source device and an ophthalmoscope device. The light source device and the ophthalmoscope device are optically connected to each other by optical fiber means adapted to transmit the light.

摘要翻译： 具有光源装置和检眼镜装置的类型的光凝器。 光源装置和检眼镜装置通过适于透射光的光纤装置彼此光学连接。

公开(公告)号：US06926915B1

公开(公告)日：2005-08-09

申请号：US09937510

申请日：2000-03-30

申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  A61K35/78

CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的量使得尽管从固相中回收的细胞与固相结合， 从液相中回收的细胞不与聚合物结合固相。