公开(公告)号：US08716018B2

公开(公告)日：2014-05-06

申请号：US12921599

申请日：2009-03-17

申请人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

发明人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

IPC分类号： C12N5/07 ,  C12N5/02

CPC分类号： C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

公开(公告)号：US08118711B2

公开(公告)日：2012-02-21

申请号：US12383446

申请日：2009-03-24

申请人： Allen Chen

发明人： Allen Chen

IPC分类号： A63B24/00

CPC分类号： A63B71/0054 ,  A63B22/0235 ,  A63B2071/0081

摘要： A treadmill is provided with a security apparatus. The security apparatus includes a plug and a socket. The plug includes a casing and a conductive card. The conductive card includes a first portion disposed in the casing and a second portion extended from the casing. The socket includes a casing and two conductive elements. The casing includes a slot defined therein. The conductive elements are disposed in the casing so that the conductive elements are isolated from each other normally but connected to each other through the conductive card when the conductive card is inserted into the casing through the slot.

摘要翻译： 跑步机设有安全装置。 安全装置包括插头和插座。 插头包括外壳和导电卡。 导电卡包括设置在壳体中的第一部分和从壳体延伸的第二部分。 插座包括壳体和两个导电元件。 壳体包括限定在其中的槽。 导电元件设置在壳体中，使得导电元件彼此正常隔离，但是当导电卡通过槽插入壳体时，导电元件通过导电卡彼此连接。

公开(公告)号：US20100093053A1

公开(公告)日：2010-04-15

申请号：US12497591

申请日：2009-07-03

申请人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

发明人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

IPC分类号： C12N11/14 ,  C12N5/02 ,  C12N11/00 ,  C12N11/12 ,  C12N11/10 ,  C12N11/02

CPC分类号： C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

公开(公告)号：US20080034211A1

公开(公告)日：2008-02-07

申请号：US11685311

申请日：2007-03-13

申请人： Mark Shull ,  Ihab Shraim ,  David Silver ,  Allen Chen ,  Elisa Cooper

发明人： Mark Shull ,  Ihab Shraim ,  David Silver ,  Allen Chen ,  Elisa Cooper

IPC分类号： H04L9/00

CPC分类号： H04L63/1441 ,  H04L29/12066 ,  H04L29/12594 ,  H04L61/1511 ,  H04L61/3015 ,  H04L63/1483 ,  H04L63/1491

摘要： Embodiments of the invention provide systems and methods for validating ownership of a domain name. According to one embodiment, a validating ownership of a domain name can comprise retrieving one or more domain name ownership records. For example, the one or more domain name ownership records comprise Who Is records. Validity of the one or more domain name records can be confirmed with a designated domain manager. According to one embodiment, confirming validity of the one or more domain name records with the designated domain manager can comprise authenticating the designated domain manager based on a certificate provided by the designated domain manager.

摘要翻译： 本发明的实施例提供用于验证域名所有权的系统和方法。 根据一个实施例，验证域名的所有权可以包括检索一个或多个域名所有权记录。 例如，一个或多个域名所有权记录包括谁是记录。 一个或多个域名记录的有效性可以用指定的域名管理员确认。 根据一个实施例，用指定的域管理器确认一个或多个域名记录的有效性可以包括基于由指定的域管理器提供的证书来认证指定的域管理者。

公开(公告)号：US07028477B2

公开(公告)日：2006-04-18

申请号：US10924877

申请日：2004-08-25

申请人： Tomoko Yoshida ,  Tetsuo Tanabe ,  Allen Chen

发明人： Tomoko Yoshida ,  Tetsuo Tanabe ,  Allen Chen

IPC分类号： B60K16/00

CPC分类号： G21H1/10

摘要： A plurality of metallic members are laminated via corresponding insulating members to form a metallic layered structure. Then, an energy beam is irradiated onto the metallic layered structure to generate an electric energy through the interactions between the metallic members of the metallic layered structure and the energy beam. The electric energy is extracted from the metallic layered structure. The metallic members are made of at least two kinds of metallic materials with respective different atomic numbers.

摘要翻译： 通过相应的绝缘构件层叠多个金属构件以形成金属层状结构。 然后，通过金属层状结构的金属构件和能量束之间的相互作用，将能量束照射到金属层状结构上以产生电能。 从金属分层结构中提取电能。 金属构件由具有不同原子序数的至少两种金属材料制成。

公开(公告)号：USD505105S1

公开(公告)日：2005-05-17

申请号：US29195691

申请日：2003-12-16

申请人： Tommaso P. Rivellini ,  Dara Sabahi ,  Jeffrey W. Umland ,  Adam D. Steltzner ,  Alejandro M. San Martin ,  William Layman ,  Edward C. Wong ,  Allen Chen ,  Robert M. Manning ,  Eric M. Slimko

设计人： Tommaso P. Rivellini ,  Dara Sabahi ,  Jeffrey W. Umland ,  Adam D. Steltzner ,  Alejandro M. San Martin ,  William Layman ,  Edward C. Wong ,  Allen Chen ,  Robert M. Manning ,  Eric M. Slimko

公开(公告)号：US20050077876A1

公开(公告)日：2005-04-14

申请号：US10924877

申请日：2004-08-25

申请人： Tomoko Yoshida ,  Tetsuo Tanabe ,  Allen Chen

发明人： Tomoko Yoshida ,  Tetsuo Tanabe ,  Allen Chen

IPC分类号： G21H1/04 ,  H05B6/62

CPC分类号： G21H1/10

摘要： A plurality of metallic members are laminated via corresponding insulating members to form a metallic layered structure. Then, an energy beam is irradiated onto the metallic layered structure to generate an electric energy through the interactions between the metallic members of the metallic layered structure and the energy beam. The electric energy is extracted from the metallic layered structure. The metallic members are made of at least two kinds of metallic materials with respective different atomic numbers.

摘要翻译： 通过相应的绝缘构件层叠多个金属构件以形成金属层状结构。 然后，通过金属层状结构的金属构件和能量束之间的相互作用，将能量束照射到金属层状结构上以产生电能。 从金属分层结构中提取电能。 金属构件由具有不同原子序数的至少两种金属材料制成。

公开(公告)号：US20170354896A1

公开(公告)日：2017-12-14

申请号：US15177772

申请日：2016-06-09

申请人： Allen CHEN

发明人： Allen CHEN

IPC分类号： A63H3/00 ,  A61H19/00

CPC分类号： A61H19/32 ,  A61H2201/0161 ,  A61H2201/0192 ,  A61H2201/168 ,  A61H2201/1685

摘要： A mannequin of a human torso adapted to retain otherwise handheld devices for simulation purposes is provided. The mannequin may have a plush shell covering filled with polymer stuffing except for a cavity therein, wherein the cavity communicates to a cavity opening defined by the shell covering. An elastic sleeve may be dimensioned and adapted to line the cavity yet protrude from the cavity opening, so that the sleeve opening is concomitant with the cavity opening. Operatively engaged along a peripheral portion of the sleeve, slightly inward for the sleeve opening, a peripheral drawstring adapted to selectively decrease the peripheral portion of the sleeve is disposed to removably retain the handheld device within the sleeve.

公开(公告)号：US09150829B2

公开(公告)日：2015-10-06

申请号：US13257674

申请日：2010-03-12

申请人： Steve Oh ,  Allen Chen ,  Andre Choo ,  Shaul Reuveny

发明人： Steve Oh ,  Allen Chen ,  Andre Choo ,  Shaul Reuveny

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2501/727 ,  C12N2531/00

摘要： A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.

摘要翻译： 公开了用于在体外培养多能细胞或多能细胞的方法，所述方法包括将多能或多潜能细胞连接到多个微载体上以形成微载体 - 细胞复合物，并在ROCK抑制剂存在下在悬浮培养物中培养微载体 - 细胞复合物 。

公开(公告)号：US08828720B2

公开(公告)日：2014-09-09

申请号：US12949172

申请日：2010-11-18

申请人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

发明人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

IPC分类号： C12N5/07 ,  C12N5/02 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12M23/20 ,  C12M25/16 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。