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公开(公告)号：US08637309B2
公开(公告)日：2014-01-28
申请号：US13210475
申请日：2011-08-16
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括基本上细长的圆柱形或棒状颗粒，其最长尺寸在50μm和400μm之间，例如约200μm。 它的横截面尺寸可以在20和30um之间。 颗粒可以包含大致为紧凑的或球形的颗粒，其尺寸为约20μm至约120μm，例如约65μm。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。
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公开(公告)号：US20110294210A1
公开(公告)日：2011-12-01
申请号：US13210475
申请日：2011-08-16
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
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公开(公告)号：US20110014693A1
公开(公告)日：2011-01-20
申请号：US12921599
申请日：2009-03-17
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。
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公开(公告)号：US08716018B2
公开(公告)日：2014-05-06
申请号：US12921599
申请日：2009-03-17
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
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公开(公告)号：US20100093053A1
公开(公告)日：2010-04-15
申请号：US12497591
申请日：2009-07-03
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。
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公开(公告)号：US20120009645A1
公开(公告)日：2012-01-12
申请号：US13257674
申请日：2010-03-12
申请人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
发明人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2501/727 , C12N2531/00
摘要： A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.
摘要翻译： 公开了用于在体外培养多能细胞或多能细胞的方法，所述方法包括将多能或多潜能细胞连接到多个微载体上以形成微载体 - 细胞复合物，并在ROCK抑制剂存在下在悬浮培养物中培养微载体 - 细胞复合物 。
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公开(公告)号：US09150829B2
公开(公告)日：2015-10-06
申请号：US13257674
申请日：2010-03-12
申请人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
发明人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
IPC分类号： C12N5/071 , C12N5/00 , C12N5/0735 , C12N5/074
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2501/727 , C12N2531/00
摘要： A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.
摘要翻译： 公开了用于在体外培养多能细胞或多能细胞的方法，所述方法包括将多能或多潜能细胞连接到多个微载体上以形成微载体 - 细胞复合物，并在ROCK抑制剂存在下在悬浮培养物中培养微载体 - 细胞复合物 。
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公开(公告)号：US08710190B2
公开(公告)日：2014-04-29
申请号：US13445705
申请日：2012-04-12
申请人： Andre Choo , Steve Oh
发明人： Andre Choo , Steve Oh
IPC分类号： C07K16/00
CPC分类号： G01N33/5073 , A61K35/545 , C07K16/28 , C12N5/0606
摘要： A method of identifying an undifferentiated human embryonic stem cell in a sample which may contain such cells, the method comprising identifying the cell or cells within the sample that express podocalyxin-like protein (PODXL) on their surface. A method of isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface. Typically, the methods use an antibody which binds to PODXL. Undifferentiated human embryonic stem cells isolated by the method may be useful in cell therapy. Also, in particular, compositions of cells differentiated from a human embryonic stem cell but which composition has been depleted of undifferentiated human embryonic stem cells are provided which are useful in cell therapy.
摘要翻译： 鉴定可能含有这种细胞的样品中未分化的人胚胎干细胞的方法，所述方法包括鉴定在其表面上表达足够胶质蛋白样蛋白（PODXL）的样品内的细胞或细胞。 从含有这种细胞的样品中分离未分化的人胚胎干细胞的方法，所述方法包括在表面上分离表达PODXL的样品中的细胞或细胞。 通常，该方法使用结合PODXL的抗体。 通过该方法分离的未分化的人胚胎干细胞可用于细胞治疗。 此外，特别地，提供了从人胚胎干细胞分化但是组合物已经耗尽未分化的人胚胎干细胞的细胞的组合物，其可用于细胞治疗。
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公开(公告)号：US08173374B2
公开(公告)日：2012-05-08
申请号：US12282164
申请日：2007-03-06
申请人： Andre Choo , Steve Oh
发明人： Andre Choo , Steve Oh
CPC分类号： G01N33/5073 , A61K35/545 , C07K16/28 , C12N5/0606
摘要： A method of identifying an undifferentiated human embryonic stem cell in a sample which may contain such cells, the method comprising identifying the cell or cells within the sample that express podocalyxin-like protein (PODXL) on their surface. A method of isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface. Typically, the methods use an antibody which binds to PODXL. Undifferentiated human embryonic stem cells isolated by the method may be useful in cell therapy. Also, in particular, compositions of cells differentiated from a human embryonic stem cell but which composition has been depleted of undifferentiated human embryonic stem cells are provided which are useful in cell therapy.
摘要翻译： 鉴定可能含有这种细胞的样品中未分化的人胚胎干细胞的方法，所述方法包括鉴定在其表面上表达足够胶质蛋白样蛋白（PODXL）的样品内的细胞或细胞。 从含有这种细胞的样品中分离未分化的人胚胎干细胞的方法，所述方法包括在表面上分离表达PODXL的样品中的细胞或细胞。 通常，该方法使用结合PODXL的抗体。 通过该方法分离的未分化的人胚胎干细胞可用于细胞治疗。 此外，特别地，提供了从人胚胎干细胞分化但是组合物已经耗尽未分化的人胚胎干细胞的细胞的组合物，其可用于细胞治疗。
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公开(公告)号：US20050233448A1
公开(公告)日：2005-10-20
申请号：US11143219
申请日：2005-06-02
申请人： Steve Oh , Andre Choo
发明人： Steve Oh , Andre Choo
IPC分类号： C12N5/0735 , C12N15/85 , C12Q1/68 , C12N5/08
CPC分类号： C12N5/0606
摘要： A method of generating stem cells, such as human and mouse embryonic stem cells, from a stem cell culture comprising the step of perfusing the stem cell culture by flowing culture media through the stem cell culture and apparatus for generating stem cells are disclosed. Also disclosed are methods and apparatus for generating stem cells, such as human and mouse embryonic cells, by culturing the cells on a plurality of hydrophilic or porous membranes. Further disclosed is a supplemented conditioned medium for obtaining improved yields of stem cells, such as human and mouse embryonic stem cells.
摘要翻译： 公开了一种从干细胞培养物产生干细胞例如人和小鼠胚胎干细胞的方法，包括通过使培养基流过干细胞培养物和用于产生干细胞的装置来灌注干细胞培养物的步骤。 还公开了通过在多个亲水或多孔膜上培养细胞来产生干细胞如人和小鼠胚胎细胞的方法和装置。 进一步公开的是用于获得干细胞如人和小鼠胚胎干细胞的提高产量的补充条件培养基。
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