公开(公告)号：US20080034211A1

公开(公告)日：2008-02-07

申请号：US11685311

申请日：2007-03-13

申请人： Mark Shull ,  Ihab Shraim ,  David Silver ,  Allen Chen ,  Elisa Cooper

发明人： Mark Shull ,  Ihab Shraim ,  David Silver ,  Allen Chen ,  Elisa Cooper

IPC分类号： H04L9/00

CPC分类号： H04L63/1441 ,  H04L29/12066 ,  H04L29/12594 ,  H04L61/1511 ,  H04L61/3015 ,  H04L63/1483 ,  H04L63/1491

摘要： Embodiments of the invention provide systems and methods for validating ownership of a domain name. According to one embodiment, a validating ownership of a domain name can comprise retrieving one or more domain name ownership records. For example, the one or more domain name ownership records comprise Who Is records. Validity of the one or more domain name records can be confirmed with a designated domain manager. According to one embodiment, confirming validity of the one or more domain name records with the designated domain manager can comprise authenticating the designated domain manager based on a certificate provided by the designated domain manager.

摘要翻译： 本发明的实施例提供用于验证域名所有权的系统和方法。 根据一个实施例，验证域名的所有权可以包括检索一个或多个域名所有权记录。 例如，一个或多个域名所有权记录包括谁是记录。 一个或多个域名记录的有效性可以用指定的域名管理员确认。 根据一个实施例，用指定的域管理器确认一个或多个域名记录的有效性可以包括基于由指定的域管理器提供的证书来认证指定的域管理者。

公开(公告)号：US20110111498A1

公开(公告)日：2011-05-12

申请号：US12949172

申请日：2010-11-18

申请人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

发明人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

IPC分类号： C12N5/0789 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12M23/20 ,  C12M25/16 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

公开(公告)号：US08691569B2

公开(公告)日：2014-04-08

申请号：US12917210

申请日：2010-11-01

申请人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

发明人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

IPC分类号： C12N5/07 ,  C12N5/02

CPC分类号： C12N5/0075 ,  C12N5/0606

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

公开(公告)号：US20120009645A1

公开(公告)日：2012-01-12

申请号：US13257674

申请日：2010-03-12

申请人： Steve Oh ,  Allen Chen ,  Andre Choo ,  Shaul Reuveny

发明人： Steve Oh ,  Allen Chen ,  Andre Choo ,  Shaul Reuveny

IPC分类号： C12N11/14 ,  C12N5/071 ,  C12N11/02 ,  C12N11/08 ,  C12N11/00 ,  C12N11/10

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2501/727 ,  C12N2531/00

摘要： A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.

摘要翻译： 公开了用于在体外培养多能细胞或多能细胞的方法，所述方法包括将多能或多潜能细胞连接到多个微载体上以形成微载体 - 细胞复合物，并在ROCK抑制剂存在下在悬浮培养物中培养微载体 - 细胞复合物 。

公开(公告)号：US20110129919A1

公开(公告)日：2011-06-02

申请号：US12917210

申请日：2010-11-01

申请人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

发明人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

IPC分类号： C12N5/071 ,  C12N5/0793 ,  C12N5/079

CPC分类号： C12N5/0075 ,  C12N5/0606

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

公开(公告)号：US20110294210A1

公开(公告)日：2011-12-01

申请号：US13210475

申请日：2011-08-16

申请人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

发明人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

IPC分类号： C12N5/071 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

公开(公告)号：US20110143433A1

公开(公告)日：2011-06-16

申请号：US12917268

申请日：2010-11-01

申请人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

发明人： Steve Oh ,  Shaul Reuveny ,  Allen Chen

IPC分类号： C12N5/0775

CPC分类号： C12N5/0606 ,  C12N5/0662 ,  C12N5/0696 ,  C12N2500/44 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2502/13 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2533/32 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2533/90

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

公开(公告)号：US20110014693A1

公开(公告)日：2011-01-20

申请号：US12921599

申请日：2009-03-17

申请人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

发明人： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

IPC分类号： C12N5/07 ,  C12N5/02

CPC分类号： C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

摘要翻译： 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

公开(公告)号：USD607531S1

公开(公告)日：2010-01-05

申请号：US29315066

申请日：2009-05-21

申请人： Allen Chen

设计人： Allen Chen

公开(公告)号：US06521485B2

公开(公告)日：2003-02-18

申请号：US09760763

申请日：2001-01-17

申请人： Spencer Su ,  James Lai ,  Lin Chien-Tsun ,  Captain Chen ,  Allen Chen ,  C.S. Yang ,  Chang Chao-Chia ,  Kevin Hsia

发明人： Spencer Su ,  James Lai ,  Lin Chien-Tsun ,  Captain Chen ,  Allen Chen ,  C.S. Yang ,  Chang Chao-Chia ,  Kevin Hsia

IPC分类号： H01L2144

CPC分类号： H01L23/3114 ,  H01L2224/05001 ,  H01L2224/05026 ,  H01L2224/05548 ,  H01L2224/16225 ,  H01L2224/32225 ,  H01L2224/73204 ,  H01L2924/00014 ,  H01L2924/00012 ,  H01L2224/05599 ,  H01L2224/05111 ,  H01L2224/05147 ,  H01L2224/05155

摘要： A method for manufacturing a wafer level chip size package and the method comprises the steps of: securing wafer to a partly etched lead frame, drilling blind hole and filling conductive material after packaging the lead frame to electrically connect the lead frame and the wafer, thus providing inner electrical connection of the wafer after packaging.