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公开(公告)号：US08883491B2
公开(公告)日：2014-11-11
申请号：US12935973
申请日：2009-04-08
申请人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
发明人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
CPC分类号： G06K9/00134 , G01N15/1475 , G01N21/64 , G01N21/6458 , G01N33/487 , G01N2015/1006 , G01N2015/1486 , G01N2015/1493 , G01N2021/6439 , G01N2201/062 , G02B21/06 , G02B21/16 , G02B21/365 , Y10T436/101666 , Y10T436/143333
摘要： The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
摘要翻译： 本发明一般涉及计数生物分子或细胞的系统和方法。 在某些实施方案中，本发明提供细胞计数或生物分子计数系统，其包括：具有已知高度并被构造成保持样品中生物分子或细胞悬浮液的被覆室; 至少一个荧光光源连接到至少一个荧光光束变窄装置; 连接到明场光束变窄装置的亮场光源; 显微镜目标; 检测装置; 荧光过滤器组件，其仅允许激发光照射样品并且仅允许来自样品的发射光被检测装置成像; 以及可动光闸以在荧光检测期间阻挡亮场光。
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公开(公告)号：US20110136152A1
公开(公告)日：2011-06-09
申请号：US12935973
申请日：2009-04-08
申请人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
发明人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
CPC分类号： G06K9/00134 , G01N15/1475 , G01N21/64 , G01N21/6458 , G01N33/487 , G01N2015/1006 , G01N2015/1486 , G01N2015/1493 , G01N2021/6439 , G01N2201/062 , G02B21/06 , G02B21/16 , G02B21/365 , Y10T436/101666 , Y10T436/143333
摘要： The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
摘要翻译： 本发明一般涉及计数生物分子或细胞的系统和方法。 在某些实施方案中，本发明提供细胞计数或生物分子计数系统，其包括：具有已知高度并被构造成保持样品中生物分子或细胞悬浮液的被覆室; 至少一个荧光光源连接到至少一个荧光光束变窄装置; 连接到明场光束变窄装置的亮场光源; 显微镜目标; 检测装置; 荧光过滤器组件，仅允许激发光照射样品并且仅允许来自样品的发射光被检测装置成像; 以及可动光闸以在荧光检测期间阻挡亮场光。
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公开(公告)号：US20100189338A1
公开(公告)日：2010-07-29
申请号：US12404803
申请日：2009-03-16
申请人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
发明人： Bo Lin , Peter Y. Li , Jean Qiu , Timothy Smith , Todd Sobolewski , Alnoor Pirani
IPC分类号： G06K9/00
CPC分类号： G01N15/1475 , G01N2015/1006 , G01N2015/1486
摘要： The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
摘要翻译： 本发明一般涉及计数生物分子或细胞的系统和方法。 在某些实施方案中，本发明提供细胞计数或生物分子计数系统，其包括：具有已知高度并被构造成保持样品中生物分子或细胞悬浮液的被覆室; 至少一个荧光光源连接到至少一个荧光光束变窄装置; 连接到明场光束变窄装置的亮场光源; 显微镜目标; 检测装置; 荧光过滤器组件，仅允许激发光照射样品并且仅允许来自样品的发射光被检测装置成像; 以及可动光闸以在荧光检测期间阻挡亮场光。
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