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公开(公告)号：US09051666B2
公开(公告)日：2015-06-09
申请号：US13617685
申请日：2012-09-14
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“装置上”组装上。
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公开(公告)号：US09023601B2
公开(公告)日：2015-05-05
申请号：US13250207
申请日：2011-09-30
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C40B50/14 , B01J19/00 , B82Y30/00 , C40B80/00 , C40B40/06
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
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公开(公告)号：US07323320B2
公开(公告)日：2008-01-29
申请号：US11201629
申请日：2005-08-10
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
IPC分类号： C12P19/34
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“装置上”组装上。
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公开(公告)号：US20130017977A1
公开(公告)日：2013-01-17
申请号：US13617685
申请日：2012-09-14
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的器件装配上。
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公开(公告)号：US07563600B2
公开(公告)日：2009-07-21
申请号：US10243367
申请日：2002-09-12
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“装置上”组装上。
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公开(公告)号：US20120270750A1
公开(公告)日：2012-10-25
申请号：US13250207
申请日：2011-09-30
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的器件装配上。
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公开(公告)号：US08058004B2
公开(公告)日：2011-11-15
申请号：US12488662
申请日：2009-06-22
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“装置上”组装上。
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公开(公告)号：US20100124767A1
公开(公告)日：2010-05-20
申请号：US12488662
申请日：2009-06-22
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
IPC分类号： C12P19/34
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装的体外合成和装配长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“装置上”组装上。
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