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1.发明申请NOVEL STRAINS OF BACTERIOPHAGES FOR THE TREATMENT OF BACTERIAL INFECTIONS, PARTICULARLY BY STRAINS OF DRUG-RESISTANT BACTERIA OF THE GENUS STENOTROPHOMONAS 审中-公开用于治疗细菌感染的特殊抗菌药物的新型菌株，特别是由基因组织耐药细菌菌株公开(公告)号：US20140193882A1
公开(公告)日：2014-07-10
申请号：US13805408
申请日：2011-06-20
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , A61K35/76 , C12N2795/00021
摘要： Bacteriophage strains and their use in the production of preparations for use in the treatment of bacterial infections, particularly by strains of drug-resistant bacteria of the genus Stenotrophomonas, particularly those belonging to the species Stenotrophomonas maltophilia
摘要翻译： 噬菌体菌株及其在生产用于治疗细菌感染的制剂中的用途，特别是由嗜麦芽寡养单宁属的耐药细菌菌株，特别是属于嗜麦芽寡养单胞菌属物种
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2.发明授权公开(公告)号：US07232564B2
公开(公告)日：2007-06-19
申请号：US10484236
申请日：2002-07-18
申请人： Beata Weber-Dabrowska , Marian Mulczyk , Andrzej Gorski , Janusz Boratynski , Marzanna Lusiak-Szelachowska , Danuta Syper
发明人： Beata Weber-Dabrowska , Marian Mulczyk , Andrzej Gorski , Janusz Boratynski , Marzanna Lusiak-Szelachowska , Danuta Syper
CPC分类号： C12N7/00 , A61K35/76 , C12N2795/00032
摘要： The multivalent strains of bacteriophages, methods of obtaining these and their use in the treatment of bacterial infections, particularly those of drug-resistant bacterial strains, especially arising in mucoviscidosis patients, are provided.
摘要翻译： 提供噬菌体的多价菌株，获得这些菌株的方法及其在治疗细菌感染中的用途，特别是耐药细菌菌株，特别是在粘液性疾病患者中产生的菌株。
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公开(公告)号：US20100227376A1
公开(公告)日：2010-09-09
申请号：US12452420
申请日：2008-06-26
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N2795/00051
摘要： New method of removing pyrogens from bacteriophage preparations based on the natural processes of endotoxin degradation, and consisting of several stages, including in particular the addition of metal ions, the heterophase extraction, and chromatography.
摘要翻译： 基于内毒素降解的天然过程从噬菌体制剂中除去热原的新方法，并且由几个阶段组成，包括特别是加入金属离子，异相萃取和色谱法。
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4.发明授权公开(公告)号：US10160956B2
公开(公告)日：2018-12-25
申请号：US13881795
申请日：2011-10-28
申请人： Krystyna Dabrowska , Anna Oslizlo , Paulina Budynek , Agnieszka Otrowicz , Andrzej Gorski , Grzegorz Figura , Barbara Owczarek , Agnieszka Kopciuch
发明人： Krystyna Dabrowska , Anna Oslizlo , Paulina Budynek , Agnieszka Otrowicz , Andrzej Gorski , Grzegorz Figura , Barbara Owczarek , Agnieszka Kopciuch
IPC分类号： C12N7/00 , C07K14/005
摘要： The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes.
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公开(公告)号：US09255251B2
公开(公告)日：2016-02-09
申请号：US12449418
申请日：2008-02-08
CPC分类号： C12N7/00 , A61K35/76 , C12N7/02 , C12N2795/00011 , C12N2795/00051 , C12N2795/10132 , C12N2795/10151
摘要： A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.
摘要翻译： 制备具有增加的抗菌活性的纯化噬菌体的方法，其中从噬菌体的细菌裂解物中获得有利的是在溶菌酶，螯合因子和洗涤剂的存在下，以连续的方式在膜上进行超滤，所述噬菌体含有高分子量制备 ，没有细菌细胞壁和其他污染物，没有毒素和内毒素，在细菌裂解试验中有活性，其特征为色谱HPLC，SDS-PAGE电泳，免疫印迹，细菌裂解生物试验，专用于噬菌体治疗 的细菌感染和肿瘤以及用于生产噬菌体衍生的药物制剂。
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6.发明申请METHOD OF PRODUCING BACTERIOPHAGE PREPARATIONS COMPRISING PURIFICATION USING AFFINITY CHROMATOGRAPHY 审中-公开使用亲和色谱法生产包含纯化的细菌培养基的方法公开(公告)号：US20130295647A1
公开(公告)日：2013-11-07
申请号：US13881795
申请日：2011-10-28
申请人： Krystyna Dabrowska , Anna Oslizlo , Paulina Budynek , Agnieszka Otrowicz , Andrzej Gorski , Grzegorz Figura , Barbara Owczarek , Agnieszka Kopciuch
发明人： Krystyna Dabrowska , Anna Oslizlo , Paulina Budynek , Agnieszka Otrowicz , Andrzej Gorski , Grzegorz Figura , Barbara Owczarek , Agnieszka Kopciuch
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C07K14/005 , C07K2319/21 , C07K2319/23 , C12N2795/00051 , C12N2795/10122 , C12N2795/10151
摘要： The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes.
摘要翻译： 所提出的方法促进用于治疗用途的噬菌体制剂的单阶段并且同时有效净化，并且在噬菌体从树脂置换及其蛋白水解释放的情况下促进噬菌体抗菌活性的维持。 使用合适的结合基序对噬菌体衣壳进行蛋白质修饰使得可以使用亲和层析纯化治疗性噬菌体菌株。 所提出的方法可用于在噬菌体衣壳上显示选择的多肽，而不需要遗传修饰噬菌体，因此使得可以使用天然存在的野生型噬菌体或其它未另外修饰用于噬菌体的其他种类的噬菌体制备各种用途的噬菌体制剂 - 显示目的。
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7.发明申请METHOD OF PRODUCING A PHARMACOLOGICALLY STABLE FORM OF LYOPHILISED, ACTIVE PREPARATIONS OF PURIFIED BACTERIOPHAGES 有权生产LYOPHILIS，活性制剂的纯化菌株的药理学稳定形式的方法公开(公告)号：US20110256103A1
公开(公告)日：2011-10-20
申请号：US13059815
申请日：2009-10-29
申请人： Ewa Zuziak , Andrzej Gamian , Andrzej Gorski , Tomasz Lipinski
发明人： Ewa Zuziak , Andrzej Gamian , Andrzej Gorski , Tomasz Lipinski
CPC分类号： C12N7/00 , A61K35/13 , C12N2795/10051 , C12N2795/10151
摘要： The subject the present invention is a method of producing a pharmacologically stable form of purified and lyophilised bacteriophage preparations of increased stability and antibacterial activity, characterised in that phages produced from a bacterial lysate, for example by ultrafiltration on ultrafiltration membranes, containing a high molecular weight preparation of purified phages is stabilised most preferably with a probiotic extract in the presence or absence of neutral salts and/or organic solvents, lyophilised, characterised via HPLC chromatography, by SDS-PAGE, and bacterial lysis biological assays, and then stored under a vacuum, where the active lyophilisate is destined for phage therapy of infections and tumours.
摘要翻译： 本发明的主题是制备具有增加的稳定性和抗菌活性的纯化和冻干的噬菌体制剂的药理稳定形式的方法，其特征在于从细菌裂解物产生的噬菌体，例如通过在超滤膜上超滤，含有高分子量 纯化噬菌体的制备在存在或不存在中性盐和/或有机溶剂的情况下最优选用益生菌提取物稳定，冻干，通过HPLC色谱法，通过SDS-PAGE表征，细菌裂解生物测定法，然后在真空下储存 ，其中活性冻干物用于感染和肿瘤的噬菌体治疗。
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公开(公告)号：US20110008873A1
公开(公告)日：2011-01-13
申请号：US12449418
申请日：2008-02-08
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , A61K35/76 , C12N7/02 , C12N2795/00011 , C12N2795/00051 , C12N2795/10132 , C12N2795/10151
摘要： A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.
摘要翻译： 制备具有增加的抗菌活性的纯化噬菌体的方法，其中从噬菌体的细菌裂解物中获得有利的是在溶菌酶，螯合因子和洗涤剂的存在下，以连续的方式在膜上进行超滤，所述噬菌体含有高分子量制备 ，没有细菌细胞壁和其他污染物，没有毒素和内毒素，在细菌裂解试验中有活性，其特征为色谱HPLC，SDS-PAGE电泳，免疫印迹，细菌裂解生物试验，专用于噬菌体治疗 的细菌感染和肿瘤以及用于生产噬菌体衍生的药物制剂。
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