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    METHOD OF PRODUCING A PHARMACOLOGICALLY STABLE FORM OF LYOPHILISED, ACTIVE PREPARATIONS OF PURIFIED BACTERIOPHAGES
    2.
    发明申请
    METHOD OF PRODUCING A PHARMACOLOGICALLY STABLE FORM OF LYOPHILISED, ACTIVE PREPARATIONS OF PURIFIED BACTERIOPHAGES 有权
    生产LYOPHILIS，活性制剂的纯化菌株的药理学稳定形式的方法

    公开(公告)号：US20110256103A1

    公开(公告)日：2011-10-20

    申请号：US13059815

    申请日：2009-10-29

    申请人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Gorski ,  Tomasz Lipinski

    发明人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Gorski ,  Tomasz Lipinski

    IPC分类号： A61K35/76 ,  A61P31/04 ,  A61P35/00

    CPC分类号： C12N7/00 ,  A61K35/13 ,  C12N2795/10051 ,  C12N2795/10151

    摘要： The subject the present invention is a method of producing a pharmacologically stable form of purified and lyophilised bacteriophage preparations of increased stability and antibacterial activity, characterised in that phages produced from a bacterial lysate, for example by ultrafiltration on ultrafiltration membranes, containing a high molecular weight preparation of purified phages is stabilised most preferably with a probiotic extract in the presence or absence of neutral salts and/or organic solvents, lyophilised, characterised via HPLC chromatography, by SDS-PAGE, and bacterial lysis biological assays, and then stored under a vacuum, where the active lyophilisate is destined for phage therapy of infections and tumours.

    摘要翻译： 本发明的主题是制备具有增加的稳定性和抗菌活性的纯化和冻干的噬菌体制剂的药理稳定形式的方法，其特征在于从细菌裂解物产生的噬菌体，例如通过在超滤膜上超滤，含有高分子量 纯化噬菌体的制备在存在或不存在中性盐和/或有机溶剂的情况下最优选用益生菌提取物稳定，冻干，通过HPLC色谱法，通过SDS-PAGE表征，细菌裂解生物测定法，然后在真空下储存 ，其中活性冻干物用于感染和肿瘤的噬菌体治疗。

    Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof
    3.
    发明授权
    Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof 有权
    噬菌体，其分离的方法，以及在其过程中有用的通用生长培养基

    公开(公告)号：US06787360B2

    公开(公告)日：2004-09-07

    申请号：US10144457

    申请日：2002-05-13

    申请人： Pushpa Agrawal ,  Vishal Soni

    发明人： Pushpa Agrawal ,  Vishal Soni

    IPC分类号： C12N1500

    CPC分类号： C12N7/00 ,  C12N1/20 ,  C12N2770/00051 ,  C12N2795/10021 ,  C12N2795/10051

    摘要： The present invention provides a isolated bacteriophage useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which comprises a novel strain of Saccharomonospora having certain specified characteristics. The invention also relates to a process for the isolation of the said bacteriophage and/or DNA phage and to a novel universal growth medium which is particularly useful in the said process. Another embodiment of the process relates to a clonong vector which comprises a plasmid or bacteriophage comprising the phage DNA of the invention.

    摘要翻译： 本发明提供了一种分离的噬菌体，其用作研究放线菌和其它生物体的生物学，生物化学，生理学和遗传学性质的工具，其包含具有某些特定特征的新型Saccharomonospora菌株。 本发明还涉及用于分离所述噬菌体和/或DNA噬菌体的方法以及在所述方法中特别有用的新型通用生长培养基。 该方法的另一个实施方案涉及一种克隆载体，其包含包含本发明的噬菌体DNA的质粒或噬菌体。

    Method of producing a pharmacologically stable form of lyophilised, active preparations of purified bacteriophages
    5.
    发明授权
    Method of producing a pharmacologically stable form of lyophilised, active preparations of purified bacteriophages 有权
    制备纯化噬菌体的冻干活性制剂的药理稳定形式的方法

    公开(公告)号：US09238798B2

    公开(公告)日：2016-01-19

    申请号：US13059815

    申请日：2009-10-29

    申请人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Górski ,  Tomasz Lipiński

    发明人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Górski ,  Tomasz Lipiński

    IPC分类号： A61K35/13 ,  C12N7/00

    CPC分类号： C12N7/00 ,  A61K35/13 ,  C12N2795/10051 ,  C12N2795/10151

    摘要： The subject the present invention is a method of producing a pharmacologically stable form of purified and lyophilised bacteriophage preparations of increased stability and antibacterial activity, characterized in that phages produced from a bacterial lysate, for example by ultrafiltration on ultrafiltration membranes, containing a high molecular weight preparation of purified phages is stabilised most preferably with a probiotic extract in the presence or absence of neutral salts and/or organic solvents, lyophilised, characterised via HPLC chromatography, by SDS-PAGE, and bacterial lysis biological assays, and then stored under a vacuum, where the active lyophilisate is destined for phage therapy of infections and tumors.

    摘要翻译： 本发明的主题是制备具有增加的稳定性和抗菌活性的纯化和冻干的噬菌体制剂的药理稳定形式的方法，其特征在于从细菌裂解物产生的噬菌体，例如通过在超滤膜上超滤，含有高分子量 纯化噬菌体的制备在存在或不存在中性盐和/或有机溶剂的情况下最优选用益生菌提取物稳定，冻干，通过HPLC色谱法，通过SDS-PAGE表征，细菌裂解生物测定法，然后在真空下储存 ，其中活性冻干物用于感染和肿瘤的噬菌体治疗。

    Bacteriophage having modified holin and uses thereof
    6.
    发明申请
    Bacteriophage having modified holin and uses thereof 审中-公开
    具有修饰的凝胶的噬菌体及其用途

    公开(公告)号：US20040156831A1

    公开(公告)日：2004-08-12

    申请号：US10714591

    申请日：2003-11-14

    发明人： Janakiraman Ramachandran ,  Sriram Padmanabhan ,  Bharathi Sriram

    IPC分类号： A61K045/00

    CPC分类号： C12N7/00 ,  A61K35/76 ,  C07K14/005 ,  C12N2795/10022 ,  C12N2795/10032 ,  C12N2795/10051 ,  Y02A50/475 ,  Y02A50/481

    摘要： The present invention features composition and methods for treating a bacterial infection using therapeutic bacteriophage having a modified holin gene. The modified holin inactivates the bacterial host prior to production of bacteriophage, so that the bacteriophage infection is non-productive, e.g., few or no bacteriophage are produced as a result of infection of the bacterial host. Thus, holin-modified bacteriophage invade the bacterial host, and cause inactivation of the bacterial host prior to production of a detectable or significant number of phage. Holin-modified phage inhibit the spread of bacterial infection without production of a significant or detectable number of phage progeny. By avoiding the release of phage progeny, the potential for generation of immune responses against the phage is reduced.

    摘要翻译： 本发明的特征在于使用具有修饰的holin基因的治疗性噬菌体治疗细菌感染的组合物和方法。 在产生噬菌体之前，修饰的蛋白激酶使细菌宿主失活，使得噬菌体感染是非生产性的，例如由于细菌宿主的感染而很少或没有产生噬菌体。 因此，霍奇修饰的噬菌体侵入细菌宿主，并在产生可检测或显着数量的噬菌体之前导致细菌宿主失活。 Holin修饰的噬菌体抑制细菌感染的扩散，而不产生显着或可检测数量的噬菌体后代。 通过避免噬菌体后代的释放，减少了针对噬菌体产生免疫应答的可能性。

    Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof
    8.
    发明授权
    Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof 有权
    噬菌体，其分离的方法，以及在其过程中有用的通用生长培养基

    公开(公告)号：US06482632B1

    公开(公告)日：2002-11-19

    申请号：US09295851

    申请日：1999-04-21

    申请人： Pushpa Agrawal ,  Vishal Soni

    发明人： Pushpa Agrawal ,  Vishal Soni

    IPC分类号： C12N700

    CPC分类号： C12N7/00 ,  C12N1/20 ,  C12N2770/00051 ,  C12N2795/10021 ,  C12N2795/10051

    摘要： The present invention provides a isolated bacteriophage useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which comprises a novel strain of Saccharomonospora having certain specified characteristics. The invention also relates to a process for the isolation of the said bacteriophage and/or DNA phage and to a novel universal growth medium which is particularly useful in the said process. Another embodiment of the process relates to a cloning vector which comprises a plasmid or bacteriophage comprising the phage DNA of the invention.

    摘要翻译： 本发明提供了一种分离的噬菌体，其用作研究放线菌和其它生物体的生物学，生物化学，生理学和遗传学性质的工具，其包含具有某些特定特征的新型Saccharomonospora菌株。 本发明还涉及用于分离所述噬菌体和/或DNA噬菌体的方法以及在所述方法中特别有用的新型通用生长培养基。 该方法的另一个实施方案涉及包含包含本发明的噬菌体DNA的质粒或噬菌体的克隆载体。