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公开(公告)号：US20070238161A1
公开(公告)日：2007-10-11
申请号：US11804151
申请日：2007-05-17
申请人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood
发明人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood
CPC分类号： G01N21/6428 , B01L3/5085 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0663 , B01L2300/1811 , C12Q1/686 , C12Q2545/101 , G01N21/0303 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/06113 , G01N2201/0628 , G01N2201/0635 , G01N2201/0636 , G01N2201/068 , C12Q2563/107
摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.
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公开(公告)号：US20060199259A1
公开(公告)日：2006-09-07
申请号：US11406148
申请日：2006-04-18
申请人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood , John Atwood
发明人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood , John Atwood
IPC分类号： C12M1/34
CPC分类号： G01N21/6428 , B01L3/5085 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0663 , B01L2300/1811 , C12Q1/686 , C12Q2545/101 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/06113 , G01N2201/0628 , G01N2201/0635 , G01N2201/0636 , G01N2201/068 , C12Q2563/107
摘要： An instrument for monitoring replication of DNA is provided.
摘要翻译： 提供了一种用于监测DNA复制的工具。
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公开(公告)号：US20070154939A1
公开(公告)日：2007-07-05
申请号：US11711416
申请日：2007-02-26
申请人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , John Atwood , Susan Stone , Judith Atwood
发明人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , John Atwood , Susan Stone , Judith Atwood
CPC分类号： G01N21/6428 , B01L3/5085 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0663 , B01L2300/1811 , C12Q1/686 , C12Q2545/101 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/06113 , G01N2201/0628 , G01N2201/0635 , G01N2201/0636 , G01N2201/068 , C12Q2563/107
摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.
摘要翻译： 光学仪器监测具有温度循环阻滞的反应装置中DNA的PCR复制，所述反应装置具有小瓶的反应成分，包括在双链DNA存在下发荧光的染料。 分束器将激发光束传递到小瓶以使染料发荧光。 来自染料的发射光束通过分束器传递到CCD检测器，处理器从该CCD检测器计算DNA浓度。 具有多个参考发射器的参考条发射不同强度的参考光束，处理器从该参考光束选择用于补偿漂移的最佳发射器。 自动调整曝光时间以保持CCD和处理器的最佳动态范围。 分束器和相关的滤光器的模块与选定的染料相关联，并且可替代不同的染料。
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公开(公告)号：US20070148761A1
公开(公告)日：2007-06-28
申请号：US11705856
申请日：2007-02-13
申请人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Judith Atwood , John Atwood , Susan Stone
发明人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Judith Atwood , John Atwood , Susan Stone
IPC分类号： C12M1/34
CPC分类号： G01N21/6428 , B01L3/5085 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0663 , B01L2300/1811 , C12Q1/686 , C12Q2545/101 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/06113 , G01N2201/0628 , G01N2201/0635 , G01N2201/0636 , G01N2201/068 , C12Q2563/107
摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having vials of reaction ingredients. A beam splitter passes an excitation beam to the vials. An emission beam from the reaction ingredients can be passed by the beam splitter to a detector from which a processor can compute DNA concentration. A reference strip with a plurality of reference emitters can be provided which emits reference beams of different intensity, from which the processor can select an optimum emitter for compensating for drift. Exposure time can be automatically adjusted for keeping within optimum dynamic ranges of the detector and processor. A module of the beam splitter and associated optical filters can be associated with a selected dye, and can be replaceable for different dyes.
摘要翻译： 光学仪器在具有小瓶反应成分的反应设备中监测DNA的PCR复制。 分束器将激发光束传递到小瓶。 来自反应成分的发射光束可以通过分光器传递到检测器，处理器可以从该分光器计算DNA浓度。 可以提供具有多个参考发射器的参考条，其发射不同强度的参考光束，处理器可以从其中选择用于补偿漂移的最佳发射器。 曝光时间可以自动调整，以保持在检测器和处理器的最佳动态范围内。 分束器和相关联的滤光器的模块可以与所选择的染料相关联，并且可以替代不同的染料。
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公开(公告)号：US20060128009A1
公开(公告)日：2006-06-15
申请号：US11333483
申请日：2006-01-17
申请人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood
发明人： Anthony Cerrone , Edward Lakatos , Michael Gambini , Eugene Young , Susan Stone , Judith Atwood
IPC分类号： C12M1/34
CPC分类号： G01N21/6428 , B01L3/5085 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0663 , B01L2300/1811 , C12Q1/686 , C12Q2545/101 , G01N21/274 , G01N21/645 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/06113 , G01N2201/0628 , G01N2201/0635 , G01N2201/0636 , G01N2201/068 , C12Q2563/107
摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.
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